排序方式: 共有17条查询结果,搜索用时 15 毫秒
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Johan Decelle Hryhoriy Stryhanyuk Benoit Gallet Giulia Veronesi Matthias Schmidt Sergio Balzano Sophie Marro Clarisse Uwizeye Pierre-Henri Jouneau Josselin Lupette Juliette Jouhet Eric Maréchal Yannick Schwab Nicole L. Schieber Rémi Tucoulou Hans Richnow Giovanni Finazzi Niculina Musat 《Current biology : CB》2019,29(6):968-978.e4
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Alexis de Ghellinck Hubert Schaller Valérie Laux Michael Haertlein Michele Sferrazza Eric Maréchal Hanna Wacklin Juliette Jouhet Giovanna Fragneto 《PloS one》2014,9(4)
Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes. 相似文献
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Dubots E Botté C Boudière L Yamaryo-Botté Y Jouhet J Maréchal E Block MA 《Biochimie》2012,94(1):86-93
Phosphatidic acid (PA) is a precursor metabolite for phosphoglycerolipids and also for galactoglycerolipids, which are essential lipids for formation of plant membranes. PA has in addition a main regulatory role in a number of developmental processes notably in the response of the plant to environmental stresses. We review here the different pools of PA dispatched at different locations in the plant cell and how these pools are modified in different growth conditions, particularly during plastid membrane biogenesis and when the plant is exposed to phosphate deprivation. We analyze how these modifications can affect galactolipid synthesis by tuning the activity of MGD1 enzyme allowing a coupling of phospho- and galactolipid metabolisms. Some mechanisms are considered to explain how physicochemical properties of PA allow this lipid to act as a central internal sensor in plant physiology. 相似文献
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Membranes of plant organelles have specific glycerolipid compositions. Selective distribution of lipids at the levels of subcellular organelles, membrane leaflets and membrane domains reflects a complex and finely tuned lipid homeostasis. Glycerolipid neosynthesis occurs mainly in plastid envelope and endoplasmic reticulum membranes. Since most lipids are not only present in the membranes where they are synthesized, one cannot explain membrane specific lipid distribution by metabolic processes confined in each membrane compartment. In this review, we present our current understanding of glycerolipid trafficking in plant cells. We examine the potential mechanisms involved in lipid transport inside bilayers and from one membrane to another. We survey lipid transfers going through vesicular membrane flow and those dependent on lipid transfer proteins at membrane contact sites. By introducing recently described membrane lipid reorganization during phosphate deprivation and recent developments issued from mutant analyses, we detail the specific lipid transfers towards or outwards the chloroplast envelope. 相似文献
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Boudière L Botté CY Saidani N Lajoie M Marion J Bréhélin L Yamaryo-Botté Y Satiat-Jeunemaître B Breton C Girard-Egrot A Bastien O Jouhet J Falconet D Block MA Maréchal E 《Molecular bioSystems》2012,8(8):2023-35, 2014
Plant cells are characterized by the presence of chloroplasts, membrane lipids of which contain up to ~80% mono- and digalactosyldiacylglycerol (MGDG and DGDG). The synthesis of MGDG in the chloroplast envelope is essential for the biogenesis and function of photosynthetic membranes, is coordinated with lipid metabolism in other cell compartments and is regulated in response to environmental factors. Phenotypic analyses of Arabidopsis using the recently developed specific inhibitor called galvestine-1 complete previous analyses performed using various approaches, from enzymology, cell biology to genetics. This review details how this probe could be beneficial to study the lipid homeostasis system at the whole cell level and highlights connections between MGDG synthesis and Arabidopsis flower development. 相似文献
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Cheong Kuan Yu Jouhet Juliette Maréchal Eric Falkowski Paul G. 《Photosynthesis research》2022,153(1-2):71-82
Photosynthesis Research - The redox state of the plastoquinone (PQ) pool is a known sensor for retrograde signaling. In this paper, we asked, “does the redox state of the PQ pool modulate the... 相似文献