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1.
Wistar rat organs and tissues were investigated after acute and chronic uremia using enzyme cytochemical means whereby special attention was paid to plasma membrane and lysosomal proteases. Heart muscle, pancreas, spleen, stomach, duodenum, jejunum, colon and skeletal muscle did not show any clear-cut indications of alterations. After acute uremia activities of dipeptidyl peptidase IV, glutamyl aminopeptidase and microsomal alanyl aminopeptidase were decreased in the extraorbital gland and that of dipeptidyl peptidase IV in the submandibular gland. The thymus showed an increased staining for glutamyl aminopeptidase and lysosomal proteases. An activity increase of dipeptidyl peptidase IV, acid phosphatase and beta-N-acetyl-D-glucosaminidase occurred in bronchial lavage cells among which the alveolar macrophages predominated. In addition, their number was comparatively higher. Non-specific esterase activity was lowered in these cells. Alkaline phosphatase activity was drastically enhanced at the biliary pole of hepatocytes. Following chronic uremia all effects were less pronounced except for the lavage cells which were positive for glutamyl aminopeptidase, microsomal alanyl aminopeptidase and gamma-glutamyl transpeptidase and showed increased staining for lysosomal proteases, glycosidases and nonspecific phosphatases. 相似文献
2.
Sinupret(?), a herbal medicinal product made from Gentian root, Primula flower, Elder flower, Sorrel herb, and Verbena herb is frequently used in the treatment of acute and chronic rhinosinusitis and respiratory viral infections such as common cold. To date little is known about its potential antiviral activity. Therefore experiments have been performed to measure the antiviral activity of Sinupret(?) oral drops (hereinafter referred to as "oral drops") and Sinupret(?) dry extract (hereinafter referred to as "dry extract"), in vitro against a broad panel of both enveloped and non-enveloped human pathogenic RNA and DNA viruses known to cause infections of the upper respiratory tract: influenza A, Chile 1/83 (H1N1) virus (FluA), Porcine Influenza A/California/07/2009 (H1N1) virus (pFluA), parainfluenza type 3 virus (Para 3), respiratory syncytial virus, strain Long (RSV), human rhinovirus B subtype 14 (HRV 14), coxsackievirus subtype A9 (CA9), and adenovirus C subtype 5 (Adeno 5). Concentration-dependent antiviral activity (EC(50) between 13.8 and 124.8 μg/ml) of Sinupret(?) was observed against RNA as well as DNA viruses independent of a viral envelope. Remarkable antiviral activity was shown against Adeno 5, HRV 14 and RSV in which dry extract was significantly superior to oral drops. This could be ascertained with different assays as plaque-reduction assays in plaque forming units (PFU), the analyses of a cytopathogenic effect (CPE) and with enzyme immunoassays (ELISA) to determine the amount of newly synthesised virus. Our results demonstrate that Sinupret(?) shows a broad spectrum of antiviral activity in vitro against viruses commonly known to cause respiratory infections. 相似文献
3.
Seidlova-Wuttke D Eder N Stahnke V Kammann M Stecher G Haunschild J Wessels JT Wuttke W 《Phytomedicine》2012,19(8-9):846-853
4.
Haunschild MD Freisleben B Takors R Wiechert W 《Bioinformatics (Oxford, England)》2005,21(8):1617-1625
MOTIVATION: Supporting the evolutionary modeling process of dynamic biochemical networks based on sampled in vivo data requires more than just simulation. In the course of the modeling process, the modeler is typically concerned not only with a single model but also with sequences, alternatives and structural variants of models. Powerful automatic methods are then required to assist the modeler in the organization and the evaluation of alternative models. Moreover, the structure and peculiarities of the data require dedicated tool support. SUMMARY: To support all stages of an evolutionary modeling process, a new general formalism for the combinatorial specification of large model families is introduced. It allows for automatic navigation in the space of models and excludes biologically meaningless models on the basis of elementary flux mode analysis. An incremental usage of the measured data is supported by using splined data instead of state variables. With MMT2, a versatile tool has been developed as a computational engine intended to be built into a tool chain. Using automatic code generation, automatic differentiation for sensitivity analysis and grid computing technology, a high performance computing environment is achieved. MMT2 supplies XML model specification and several software interfaces. The performance of MMT2 is illustrated by several examples from ongoing research projects. AVAILABILITY: http://www.simtec.mb.uni-siegen.de/ CONTACT: wiechert@simtec.mb.uni-siegen.de. 相似文献
5.
Summary Wistar rat organs and tissues were investigated after acute and chronic uremia using enzyme cytochemical means whereby special attention was paid to plasma membrane and lysosomal proteases. Heart muscle, pancreas, spleen, stomach, duodenum, jejunum, colon and skeletal muscle did not show any clear-cut indications of alterations. After acute uremia activities of dipeptidyl peptidase IV, glutamyl aminopeptidase and microsomal alanyl aminopeptidase were decreased in the extraorbital gland and that of dipeptidyl peptidase IV in the submandibular gland. The thymus showed and increased staining for glutamyl aminopeptidase and lysosomal proteases. An activity increase of dipeptidyl peptidase IV, acid phosphatase and -N-acetyl-d-glucosaminidase occurred in bronchial lavage cells among which the alveolar macrophages predominated. In addition, their number was comparatively higher. Non-specific esterase activity was lowered in these cells. Alkaline phosphatase activity was drastically enhanced at the biliary pole of hepatocytes. Following chronic uremia all effects were less pronounced except for the lavage cells which were positive for glutamyl aminopeptidase, microsomal alanyl aminopeptidase and -glutamyl transpeptidase and showed increased staining for lysosomal proteases, glycosidases and nonspecific phosphatases.Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthdaySupported by the German Research Foundation (Sfb 174) 相似文献
6.
J. Haunschild K. Steiner H-P. Faro R. Senekowitsch 《Cell biochemistry and biophysics》1995,26(3):167-182
The murine monoclonal antibody (MAb) 425 (mMAb 425) directed against the human EGFR (epidermal growth factor receptor) was reshaped (rMAb 425) in order to improve its therapeutical potential in humans. The pharmacokinetic properties of [125I]-mMAb and [125I]-rMAb 425 were compared in three animal species. Whereas the clearance curves of both antibodies decreased biphasically in rats and nude mice bearing human mammary carcinoma, a monophasic decline was observed in Cynomolgus monkeys. Plasma elimination half-lives of murine and reshaped MAb 425 were similar, short in the monkey (26 h for mMAb 425 and 31 h for rMAb 425) and long in rats (240 h for mMAb 425 and 225 h for rMAb 425). In xenografted nude mice however, the half-life of mMAb 425 (203 h) was about twice as long as that of rMAb 425 (124 h). The half-lives of intact rMAb 425 in the three species obtained by ELISA differed at most by a factor of two from those obtained by radioactivity measurements. Biodistribution studies of [125I]-rMAb 425 revealed a tumor/blood ratio of 1.2 on d 1 and 5.1 on d 18, respectively. Fifty-four and thirty-eight percent of the radioactive dose were excreted with urine in nude mice (within 12 d) and rats (within 11 d), respectively. Specific localization of [125I]-rMAb 425 in human mammary carcinoma xenografted to nude mice was demonstrated. 相似文献
7.
Harold L. Leverenz Kristine Haunschild Guy Hopes George Tchobanoglous Jeannie L. Darby 《Ecological Engineering》2010,36(11):1544-1551
Anoxic subsurface flow (SSF) constructed wetlands were evaluated for denitrification using nitrified wastewater. The treatment wetlands utilized a readily available organic woodchip-media packing to create the anoxic conditions. After 2 years in operation, nitrate removal was found to be best described by first-order kinetics. Removal rate constants at 20 °C (k20) were determined to be 1.41–1.30 d?1, with temperature coefficients (θ) of 1.10 and 1.17, for planted and unplanted experimental woodchip-media SSF wetlands, respectively. First-order removal rate constants decreased as length of operation increased; however, a longer-term study is needed to establish the steady-state values. The hydraulic conductivity in the planted woodchip-media SSF wetlands, 0.13–0.15 m/s, was similar to that measured in an unplanted gravel-media SSF control system. 相似文献
8.
R. Senekowitsch-Schmidtke Kurt Steiner Jutta Haunschild Sigrun Möllenstädt Regine Truckenbrodt 《Cancer immunology, immunotherapy : CII》1996,42(2):108-114
In order to study the potential of non-invasive scintigraphic evaluation of the epidermal growth factor (EGF) receptor status
in vivo, the biokinetics and tumor binding of 125I-EGF and anti-(EGF receptor) mAb 425 were investigated in nude mice bearing human tumor xenografts with different EGF-receptor
densities as determined by a radioreceptor assay. The results demonstrated a tumor uptake for both substances depending on
the receptor level. The EGF receptor status, however, was reflected slightly better by the binding of EGF to tumor tissue
compared to the mAb. The rapid blood clearance of EGF with a plasma half-life of less than 1 min led to a tumor-to-blood ratio
of approximately 3 within 6 h after injection in tumors with a high receptor expression. A similar ratio for the mAb was not
obtained before day 6 after injection. The absolute concentration of EGF, however, was low compared to the mAb. Therefore,
it can be concluded that the EGF receptor status as a target for (radio)immunotherapy can be evaluated in vivo with EGF labeled
with a short-life positron-emitting radionuclide or with monoclonal antibodies to the EGF receptor or their fragments.
Received: 14 September 1995 / Accepted: 6 December 1995 相似文献
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10.
Aida Salameh Josphina Haunschild Paul Br?uchle Oliver Peim Thomas Seidel Marko Reitmann Martin Kostelka Farhad Bakhtiary Stefan Dhein Ingo D?hnert 《PloS one》2014,9(4)