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1.
Changes in ergosterol content in cultures of Penicillium brevicompactum and Aspergillus versicolor on wood with time, changes in humidity or addition of glucose solutions to wood were studied with HPLC. Lowering of the humidity level caused a very large decline in ergosterol content of cultures of P. brevicompactum on wood over a 10 day period, although small amounts remained after this time. After an initial increase, up to an inoculation time of 45 days, reductions were also observed in control samples maintained at 100% RH, but these were smaller. The amount of ergosterol decreased to very low levels in wood impregnated with low levels of glucose during a 93 day incubation period. Ergosterol concentration in hyphae produced in surface liquid cultures was shown to be higher in mycelia growing on media enriched with nitrogen or with more available nutrients. The concentration of ergosterol in the mycelia of P. brevicompactum in surface liquid cultures varied by a factor of 5 from 2 to 10 mg g−. The results clearly show that ergosterol present in solid materials in mainly related to active biomass. With certain prerequisites, ergosterol determinations could also be used for total fungal biomass estimations on wood. 相似文献
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Glycolipid-phospholipid vesicles containing phosphatidate and phosphatidylethanolamine were found to undergo proton-induced fusion upon acidification of the suspending medium from pH 7.4 to pH 6.5 or lower, as determined by an assay for lipid intermixing based on fluorescence resonance energy transfer. Lectinmediated contact between the vesicles was required for fusion. Incorporation of phosphatidylcholine in the vesicles inhibited proton-induced fusion. Vesicles in which phosphatidate was replaced by phosphatidylserine underwent fusion only when pH was reduced below 4.5, while no significant fusion occured (pH ? 3.5) when the anionic phospholipid was phosphatidylinositol. It is suggested that partial protonation of the polar headgroup of phosphatidate and phosphatidylserine, respectively, causes a sufficient reduction in the polarity and hydration of the vesicle surface to trigger fusion at sites of intermembrane contact. 相似文献
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Patricia Kreis Rita Hendricusdottir Louise Kay Ismini E. Papageorgiou Michiel van Diepen Till Mack Jonny Ryves Adrian Harwood Nicholas R. Leslie Oliver Kann Maddy Parsons Britta J. Eickholt 《PloS one》2013,8(8)
Defects in actin dynamics affect activity-dependent modulation of synaptic transmission and neuronal plasticity, and can cause cognitive impairment. A salient candidate actin-binding protein linking synaptic dysfunction to cognitive deficits is Drebrin (DBN). However, the specific mode of how DBN is regulated at the central synapse is largely unknown. In this study we identify and characterize the interaction of the PTEN tumor suppressor with DBN. Our results demonstrate that PTEN binds DBN and that this interaction results in the dephosphorylation of a site present in the DBN C-terminus - serine 647. PTEN and pS647-DBN segregate into distinct and complimentary compartments in neurons, supporting the idea that PTEN negatively regulates DBN phosphorylation at this site. We further demonstrate that neuronal activity increases phosphorylation of DBN at S647 in hippocampal neurons in vitro and in ex vivo hippocampus slices exhibiting seizure activity, potentially by inducing rapid dissociation of the PTEN:DBN complex. Our results identify a novel mechanism by which PTEN is required to maintain DBN phosphorylation at dynamic range and signifies an unusual regulation of an actin-binding protein linked to cognitive decline and degenerative conditions at the CNS synapse. 相似文献
4.
Julia Beaumont Jonny Geber Natasha Powers Andrew Wilson Julia Lee‐Thorp Janet Montgomery 《American journal of physical anthropology》2013,150(1):87-98
Historical evidence documents mass migration from Ireland to London during the period of the Great Irish Famine of 1845–52. The rural Irish were reliant on a restricted diet based on potatoes but maize, a C4 plant, was imported from the United States of America in 1846–47 to mitigate against Famine. In London, Irish migrants joined a population with a more varied diet. To investigate and characterize their diet, carbon and nitrogen isotope ratios were obtained from bone collagen of 119 and hair keratin of six individuals from Lukin Street cemetery, Tower Hamlets (1843–54), and bone collagen of 20 individuals from the cemetery at Kilkenny Union Workhouse in Ireland (1847–51). A comparison of the results with other contemporaneous English populations suggests that Londoners may have elevated δ15N compared with their contemporaries in other cities. In comparison, the Irish group have lower δ15N. Hair analysis combined with bone collagen allows the reconstruction of perimortem dietary changes. Three children aged 5–15 years from Kilkenny have bone collagen δ13C values that indicate consumption of maize (C4). As maize was only imported into Ireland in quantity from late 1846 and 1847, these results demonstrate relatively rapid bone collagen turnover in children and highlight the importance of age‐related bone turnover rates, and the impact the age of the individual can have on studies of short‐term dietary change or recent migration. Stable light isotope data in this study are consistent with the epigraphic and documentary evidence for the presence of migrants within the London cemetery. Am J Phys Anthropol, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
5.
Nogueira Gabriela Ferreira Luis Zanderluce Gomes Pasqual Moacir Scherwinski-Pereira Jonny Everson 《In vitro cellular & developmental biology. Plant》2019,55(1):26-35
In Vitro Cellular & Developmental Biology - Plant - The current study assessed the embryogenic potential of different Brazilian Saccharum spp. hybrid (sugarcane) varieties, using explants from... 相似文献
6.
Ferreira Jéssica Cristina Barbosa de Araújo Silva-Cardoso Inaê Mariê Meira Rennan Oliveira da Silva Costa Frederico Henrique Scherwinski-Pereira Jonny Everson 《In vitro cellular & developmental biology. Plant》2022,58(5):750-768
In Vitro Cellular & Developmental Biology - Plant - An efficient, reproducible, and unprecedented protocol of somatic embryogenesis (SE) was developed from leaf tissues of adult plants of... 相似文献
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Randomized controlled trial of a nationally available weight control program tailored for adults with type 2 diabetes 下载免费PDF全文
Patrick M. O'Neil Peter W. Tuerk Lynne E. Becker Thomas A. Wadden Ken Fujioka Priscilla L. Hollander Robert F. Kushner W. Timothy Garvey Domenica M. Rubino Robert J. Malcolm Daniel Weiss William J. Raum Jonny L. Salyer Kathie L. Hermayer Jan L. Veliko Nicoleta D. Sora 《Obesity (Silver Spring, Md.)》2016,24(11):2269-2277