全文获取类型
收费全文 | 4666篇 |
免费 | 336篇 |
国内免费 | 1篇 |
出版年
2023年 | 29篇 |
2022年 | 63篇 |
2021年 | 129篇 |
2020年 | 53篇 |
2019年 | 87篇 |
2018年 | 113篇 |
2017年 | 136篇 |
2016年 | 177篇 |
2015年 | 242篇 |
2014年 | 283篇 |
2013年 | 340篇 |
2012年 | 429篇 |
2011年 | 433篇 |
2010年 | 237篇 |
2009年 | 175篇 |
2008年 | 259篇 |
2007年 | 284篇 |
2006年 | 258篇 |
2005年 | 271篇 |
2004年 | 231篇 |
2003年 | 198篇 |
2002年 | 180篇 |
2001年 | 54篇 |
2000年 | 21篇 |
1999年 | 30篇 |
1998年 | 45篇 |
1997年 | 21篇 |
1996年 | 18篇 |
1995年 | 22篇 |
1994年 | 12篇 |
1993年 | 16篇 |
1992年 | 9篇 |
1991年 | 9篇 |
1990年 | 11篇 |
1989年 | 6篇 |
1988年 | 12篇 |
1987年 | 15篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 6篇 |
1977年 | 6篇 |
1976年 | 7篇 |
1973年 | 4篇 |
1970年 | 3篇 |
1968年 | 3篇 |
1967年 | 3篇 |
排序方式: 共有5003条查询结果,搜索用时 31 毫秒
1.
Background
Radial chromosome positioning in interphase nuclei is nonrandom and can alter according to developmental, differentiation, proliferation, or disease status. However, it is not yet clear when and how chromosome repositioning is elicited. 相似文献2.
3.
Joanna Nakonieczna Ewelina Michta Magda Rybicka Mariusz Grinholc Anna Gwizdek-Wiśniewska Krzysztof P Bielawski 《BMC microbiology》2010,10(1):323
Background
Staphylococcus aureus, a major human pathogen causes a wide range of disease syndromes. The most dangerous are methicillin-resistant S. aureus (MRSA) strains, resistant not only to all β-lactam antibiotics but also to other antimicrobials. An alarming increase in antibiotic resistance spreading among pathogenic bacteria inclines to search for alternative therapeutic options, for which resistance can not be developed easily. Among others, photodynamic inactivation (PDI) of S. aureus is a promising option. Photodynamic inactivation is based on a concept that a non toxic chemical, called a photosensitizer upon excitation with light of an appropriate wavelength is activated. As a consequence singlet oxygen and other reactive oxygen species (e.g. superoxide anion) are produced, which are responsible for the cytotoxic effect towards bacterial cells. As strain-dependence in photodynamic inactivation of S. aureus was observed, determination of the molecular marker(s) underlying the mechanism of the bacterial response to PDI treatment would be of great clinical importance. We examined the role of superoxide dismutases (Sod) in photodynamic inactivation of S. aureus as enzymes responsible for oxidative stress resistance. 相似文献4.
Nathan Downs Alfio Parisi Joanna Turner David Turnbull 《Photochemical & photobiological sciences》2008,7(6):700-710
A technique has been developed to represent erythemally effective ultraviolet radiation exposure within a school environment. The technique models the erythemally effective exposure onto a horizontal plane representation of a mapped school environment located in Hervey Bay (25 degrees S, 153 degrees E), Australia. The input parameters used to model the ultraviolet exposures received within the school playground included the measured sky view, ground albedo and standing surface albedo. Estimates of the erythemally effective ultraviolet exposure received within the school playground during morning tea and lunch time meal breaks during a winter and summer school day are presented. The influence of tree shade and building structure was found to vary significantly with solar zenith angle modelled over the winter and summer school meal break times with horizontal plane exposures predicted to vary from between 0 and 7 SED at different locations within the playground. The technique presented provides a method that can be followed to examine the effect of surrounding buildings and surface structures of real environments on the predicted horizontal plane ultraviolet exposure. 相似文献
5.
Carroll D. Arnett Joanna S. Fowler Robert R. MacGregor David J. Schlyer Alfred P. Wolf Bengt Långström Christer Halldin 《Journal of neurochemistry》1987,49(2):522-527
The distribution of carbon-11-labeled L-deprenyl, an irreversible inhibitor of monoamine oxidase type B (MAO-B), was determined in the baboon brain by positron emission tomography. The irreversible blood-to-brain transfer constant (influx constant, Ki) was measured using a complete metabolite-corrected arterial plasma concentration curve. This influx constant was used as a measure of functional enzyme activity for sequential determinations of MAO-B recovery following a single high dose of unlabeled l -deprenyl. The half-life for turnover of MAO-B was thus determined to be 30 days. Using appropriate irreversible inhibitors, this procedure should be generally useful for determining enzyme turnover rates in any organ in vivo and can be applied to some human studies as well. 相似文献
6.
Insulin signal transmission through the plasma membrane was studied in terms of relationship between basal autophosphorylation of the β-subunit and the ability by bind insulin by the -subunit of the insulin receptor. In a cell free system, receptors phosphorylated on tyrosine residues in the absence of insulin were separated from non-phosphorylated receptors using antiphosphotyrosine antibodies. Insulin binding assays were then performed on basally autophosphorylated and on non-phosphorylated receptors. We found that the tyrosine phosphorylated receptors, which corresponded to 25% of the total number of receptors, were accountable for 60–80% of insulin binding. Scatchard representation of binding data has shown that the plot corresponding to tyrosine phosphorylated receptors was localized above, and was steeper than the plot corresponding to non-phosphorylated receptors. These data make it likely that the conformation of -subunit which favours ligand binding is connected to the conformation of β-subunit which favours phosphate reception on tyrosine residues. Reciprocally, the high-affinity conformation of insulin receptor seems to become stabilized by basal autophosphorylation. 相似文献
7.
A rapid method for measuring the in vitro attachment of Candida albicans to the surface of transparent acrylic is described. The method involves use of the 'Magiscan' automated image analysis system which measures attachment in terms of percentage area coverage. These measurements correlate highly significantly ( P < 0·001) with the number of adherent yeast cells. 相似文献
8.
Prevalence of the 281 (Gly→Glu) mutation in hepatoerythropoietic porphyria and porphyria cutanea tarda 总被引:4,自引:0,他引:4
Hubert de Verneuil Joanna Hansen Christiane Picat Bernard Grandchamp James Kushner Andrew Roberts George Elder Yves Nordmann 《Human genetics》1988,78(1):101-102
Summary The prevalence of the 281 (GlyGlu) mutation in hepatoerythropoietic porphyria (HEP) was investigated by the use of hybridization with a synthetic oligonucleotide probe. The mutation was found in HEP-affected members of two unrelated families from Spain, but was absent in two other patients from Italy and Portugal who also had HEP. Moreover, this mutation was not detected in 13 unrelated cases of familial (type II) porphyria cutanea tarda. 相似文献
9.
The Lindero membranaceae-Fagetum crenatae association on Mt. Sanpoiwadake, Hakusan National Park, Japan, has been classified
using traditional Braun-Blanquet methods. The association was floristically sub-divided into six communities, all of which
had been included as four subassociations by Hukusima (1982). The resulting table was compared with an analogous community
table derived using computer-based TWINSPAN analysis. This dual approach was shown to provide a fully objective classification
by method, the subjective element in the Braun-Blanquet method thus being substantiated TWINSPAN. Subsequent ordination of
the data using DCA then provided a further degree of objective evaluation allowing any misclassified stand or species to be
re-examined, and at the same time displaying the detailed inter-relationships between all species or stands. 相似文献
10.
Detailed examination of a sample plot covered by the Lindero membranaceae-Fagetum crenatae association on Mt. Sanpoiwadake,
Hakusan National Park, revealed a number of correlations between the distribution of subassociations and environmental factors.
The subassociations on the south-facing slopes receive deep snow cover in winter with rapid melting in the spring. They occur
on porous, freely draining soils, typical of the general range of brown forest soils. Conversely, on the north-eastern slopes
there are widespread late-snow patches which delay leaf development and expansion and which provide an abundant water supply
well into early summer. Under these conditions, bleached soil horizons have developed with iron pan formation, resulting in
poor soil drainage, strongly correlated with quite different plant communities. 相似文献