排序方式: 共有11条查询结果,搜索用时 15 毫秒
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Cui Liwei Zhao Jiating Chen Jiuyu Zhang Wei Gao Yuxi Li Bai Li Yu-Feng 《Plant and Soil》2018,425(1-2):577-588
Plant and Soil - Cardamine enshiensis (C. enshiensis) is a selenium (Se) hyperaccumulator plant found in Enshi, a typical seleniferous area in southwestern Hubei Province, central China. The aim of... 相似文献
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为了筛选大白猪GABRR2基因的SNP和对GABRR2基因进行结构、功能的预测和分析,本研究利用30头大白猪混池为模板,通过PCR扩增GABRR2基因外显子,测序后进行SNP筛选,并对测序结果进行拼接,利用多种生物信息学软件对大白猪GABRR2基因启动子区、编码区、3'UTR区进行了一系列的分析.结果显示,大白猪GABRR2基因在c615、c660、c798和c1134共有4个同义突变;RNGTT、SRSF12和ANKRD63个基因在GABRR2基因连锁区间内,且编码的蛋白具有相互作用;起始密码子上游2 000bp区域在385~435号碱基和759~806号碱基区域有2个核心启动子区域,在核心启动子386~395、769~778有Sp1转录因子结合位点,757~766有CREB-2转录因子结合位点,775~784有NF-1转录因子结合位点,在1 593~1 752号碱基区间内有一个 CpG 岛;3'UTR 区域有 14个 miRNA 结合位点,其中 miR-365-3p、miR-497-5p、miR-5195-3p 和miR-145-5p评分高于80.研究结果表明,大白猪GABRR2基因CDS区全长1 380 bp,存在4个同义突变,保守性高;编码一个具有4个跨膜结构、1个信号肽的亲水性蛋白,与RNGTT基因功能可能有密切联系;2个核心启动子区域内含有Sp1、CREB-2和NF-1三种共4个转录因子结合位点,且启动子区有一个特征的CpG岛;3'UTR 区域存在 miR-365-3p、miR-497-5p、miR-5195-3p 和 miR-145-5p 共4个 miRNA 结合区域. 相似文献
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Lilan You Jun Ma Jiuyu Wang Daria Artamonova Min Wang Liang Liu Hua Xiang Konstantin Severinov Xinzheng Zhang Yanli Wang 《Cell》2019,176(1-2):239-253.e16
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Liu R Gong J Chen J Li Q Song C Zhang J Li Y Liu Z Dong Y Chen L Jin B 《Cancer immunology, immunotherapy : CII》2012,61(6):855-864
Calreticulin (CRT) is an endoplasmic reticulum luminal Ca(2+)-binding chaperone protein. By immunizing mice with recombinant fragment (rCRT/39-272), six clones of monoclonal antibodies (mAbs) were generated and characterized. Based on these mAbs, a microplate chemiluminescent enzyme immunoassay (CLEIA) system with a measured limit of detection of 0.09?ng/ml was developed. Using this CLEIA system, it was found that soluble CRT (sCRT) level in serum samples from 58 lung cancer patients was significantly higher than that from 40 healthy individuals (only 9 were detectable, P?0.0001). Among them, serum sCRT in the small cell lung cancer was lower than that in adenocarcinoma (P?=?0.0085), while both were lower than that in the squamous cell carcinoma (P?=?0.013, P?=?0.0012, respectively). Moreover, it was found that sCRT in sera from the patients after chemotherapy was higher than that from the patients without chemotherapy (P?=?0.042). Further study by immunohistochemistry showed that CRT was also highly expressed in the cytoplasm and on the membrane of the lung cancer cells, while there was a trace amount of CRT expression in normal lung cells. Correspondingly, the expression level of CRT on lung cancer cell membrane was associated with the tumor pathological grade. This study demonstrates that sCRT concentration in sera of lung cancer patients is higher than that in sera of healthy individuals, and CRT expression level on lung cancer cell membrane is associated with tumor pathological classification and grade. These findings suggest that CRT may be used as a biomarker in lung cancer prediction and diagnosis. 相似文献
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Yang Jing Li Jiazhi Wang Jiuyu Sheng Gang Wang Min Zhao Hongtu Yang Yanhua Wang Yanli 《中国科学:生命科学英文版》2020,63(4):516-528
Cas1 is a key component of the CRISPR adaptation complex, which captures and integrates foreign DNA into the CRISPR array,resulting in the generation of new spacers. We have determined crystal structures of Thermus thermophilus Cas1 involved in new spacer acquisition both in complex with branched DNA and in the free state. Cas1 forms an asymmetric dimer without DNA.Conversely, two asymmetrical dimers bound to two branched DNAs result in the formation of a DNA-mediated tetramer, dimer of structurally asymmetrical dimers, in which the two subunits markedly present different conformations. In the DNA binding complex, the N-terminal domain adopts different orientations with respect to the C-terminal domain in the two monomers that form the dimer. Substrate binding triggers a conformational change in the loop 164–177 segment. This loop is also involved in the 3′ fork arm and 5′ fork arm strand recognition in monomer A and B, respectively. This study provides important insights into the molecular mechanism of new spacer adaptation. 相似文献
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The binding of selenite, the form of selenium used in the treatment of Keshan disease, to plasma proteins and the role of erythrocytes in this process have been studied. The experiments were carried out by incubating75Se as selenite with plasma and whole bloodin vitro (human and rat) andin vivo (rat) and subsequent fractionation by Sephadex G-150 gel filtration. Human and rat plasma proteins were unable to incorporate selenium from selenite, as shown by the negligible amount present in proteins after incubation of plasma with selenite. The incorporation can be carried out after internalization of selenite by the erythrocytes in a fast, temperature dependent process. Hemoglobin, being the major binding protein for the newly reduced selenium in the erythrocyte, might have a role in the uptake of selenite by erythrocytes. The greater affinity of plasma proteins for the final selenium compound resulting from reduction could be the cause of the efflux. However, this is minimal in the absence of plasma, as is evident from the results obtained from blood reconstituted with saline solution instead of plasma. At least two proteins, one albumin like, probably albumin itself and the other of molecular weight close to or greater than 200,000 in the plasma, are involved in the binding and efflux processes. 相似文献
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