全文获取类型
收费全文 | 106篇 |
免费 | 0篇 |
国内免费 | 15篇 |
出版年
2023年 | 4篇 |
2022年 | 4篇 |
2021年 | 11篇 |
2020年 | 8篇 |
2019年 | 4篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 7篇 |
2014年 | 9篇 |
2013年 | 10篇 |
2012年 | 8篇 |
2011年 | 10篇 |
2010年 | 8篇 |
2009年 | 4篇 |
2008年 | 6篇 |
2007年 | 4篇 |
2006年 | 1篇 |
2005年 | 1篇 |
2004年 | 3篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有121条查询结果,搜索用时 15 毫秒
1.
Hüiwan Han Dali Shao Jinyi Wu Germaine Cornélissen Franz Halberg 《Cell biochemistry and biophysics》1991,18(3):217-229
An earlier demonstration of a circadian rhythm in rat atria by others is complemented herein by observations in culture: A single murine myocardial cell and two sets of grouped cells beating in culture for several days reveal several features of an anticipated, presumably built-in spectrum of multifrequency rhythms and trends, the chronome. Circadian and about 12-h (circasemidian) components are modulated by an approximately 84-h (circasemiseptan) component, which cannot be separated from trends in view of the brevity of the series. The circumstance under which the culture is aging and in which fibroblasts proliferate is a further complication that limits the findings to a single cycle reproduced in three separate cultures. Whether it is a rhythm that repeats itself or a response to placement into culture, an approximately 3.5-d component in the beating of myocardial cells in culture is to be aligned with a very prominent similar component found in the incidence of 85,819 human myocardial infarctions. 相似文献
2.
Gao Kai Liu Meiyou Li Yuan Wang Lei Zhao Chao Zhao Xian Zhao Jinyi Ding Yi Tang Haifeng Jia Yanyan Wang Jingwen Wen Aidong 《Journal of molecular histology》2021,52(3):449-459
Journal of Molecular Histology - Currently, the excessive activation of N-methyl-D-aspartate receptors (NMDARs) is considered to be a crucial mechanism of brain injury. Lycium barbarum A (LyA) is a... 相似文献
3.
Halberg F Cornélissen G Stoynev A Ikonomov O Katinas G Sampson M Wang Z Wan C Singh RB Otsuka K Sothern RB Sothern SB Sothern MI Syutkina EV Masalov A Perfetto F Tarquini R Maggioni C Kumagai Y Siegelova J Fiser B Homolka P Dusek J Uezono K Watanabe Y Wu J Prikryl P Blank M Blank O Sonkowsky R Schwartzkopff O Hellbrügge T Spector NH Baciu I Hriscu M Bakken E 《Neuro endocrinology letters》2003,24(6):479-498
4.
Rapid and reliable detection of 11 food-borne pathogens using thin-film biosensor chips 总被引:1,自引:0,他引:1
Sulan Bai Jinyi Zhao Yaochuan Zhang Wensheng Huang Shi Xu Haodong Chen Liu-Min Fan Ying Chen Xing Wang Deng 《Applied microbiology and biotechnology》2010,86(3):983-990
Traditional methods for identifying food-borne pathogens are time-consuming and laborious, so it is necessary to develop innovative
methods for the rapid identification of food-borne pathogens. Here, we report the development of silicon-based optical thin-film
biosensor chips for sensitive detection of 11 food-borne pathogens. Briefly, aldehyde-labeled probes were arrayed and covalently
attached to a hydrazine-derivatized chip surface, and then, biotinylated polymerase chain reaction (PCR) amplicons were hybridized
with the probes. After washing and brief incubation with an antibiotin immunoglobulin G–horseradish peroxidase conjugate and
a precipitable horseradish peroxidase substrate, biotinylated chains bound to the probes were visualized as a color change
on the chip surface (gold to blue/purple). Highly sensitive and accurate examination of PCR fragment targets can be completed
within 30 min. This assay is extremely robust, sensitive, specific, and economical and can be adapted to different throughputs.
Thus, a rapid, sensitive, and reliable technique for detecting 11 food-borne pathogens was successfully developed. 相似文献
5.
In this study, a high-throughput microfluidic system is presented. The system is comprised of seven parallel channels. Each channel contains 32 square-shaped microchambers. After simulation studies on samples loaded into the microchambers, and the solute exchange between the microchambers and channels, the long-term culture of Escherichia coli (E. coli) HB101 in the microchambers is realized. Using the principle that L-arabinose (L-Ara) can induce recombinant E. coli HB101 pGLO to synthesize green fluorescent protein (GFP), the real-time analysis of GFP expression in different initial bacterial densities is performed. The results demonstrate that higher initial loading densities of the bacterial colony cause bacterial cell to enter log-phase proliferation sooner. High or low initial loading densities of the bacterial cell suspension induce the same maximum growth rates during the log-phase. Quantitative on-chip analysis of tetracycline and erythromycin inhibition on bacterial cell growth is also conducted. Bacterial morphology changes during antibiotic treatment are observed. The results show that tetracycline and erythromycin exhibit different inhibition activities in E. coli cells. Concentrations of 3 μg/mL tetracycline can facilitate the formation of long filamentous bacteria with the average length of more than 50 μm. This study provides an on-chip framework for bacteriological research in a high-throughput manner and the development of recombinant bacteria-based biosensors for the detection of specific substances. 相似文献
6.
7.
8.
三种重要单宁植物营养器官中单宁的分布(简报) 总被引:5,自引:0,他引:5
余甘子(PhyllanthusembnlicaL.)、杨梅(Myricarubra(Lour.)Sieb.etZucc.X细枝木麻黄(CasaarinacunninghamianaMiq.)是重要的单宁植物。Moseley[1]咱报道木麻黄属茎的次生木质部和次生韧皮部的结构。Metcalf和ChalkP[2]曾对这三种植物所在属的营养器官结构作了概述,但未涉及这三种植物。Sidey[3]曾报道柔毛金合欢(ACaciamollissimaWilld)各器官的组织中单宁的分布。我们观察了上述三种植物根、茎、叶(或同化技)组织中单宁的分布,为研究植物体内单宁的分布提供资料,同时为生产上充分提取单宁提供理论依据.1材料和方… 相似文献
9.
Fei Ling Huan Zhang Yunliang Sun Jinyi Meng Jaceline Gislaine Pires Sanches He Huang Qingqing Zhang Xiao Yu Bo Wang Li Hou Jun Zhang 《Cell death & disease》2021,12(11)
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and metastasis is the major cause of the high mortality of HCC. In this study, we identified that AnnexinA7 (ANXA7) and Sorcin (SRI) are overexpressed and interacting proteins in HCC tissues and cells. In vitro functional investigations revealed that the interaction between ANXA7 and SRI regulated epithelial–mesenchymal transition (EMT), and then affected migration, invasion, and proliferation in HCC cells. Furthermore overexpression/knockdown of ANXA7 was remarkably effective in promoting/inhibiting tumorigenicity and EMT in vivo. Altogether, our study unveiled a mechanism that ANXA7 promotes EMT by interacting with SRI and further contributes to the aggressiveness in HCC, which provides a novel potential therapeutic target for preventing recurrence and metastasis in HCC.Subject terms: Medical research, Genetics research 相似文献
10.
Dewen Tong Jinyi Wang Peihong Mu Qiang Dong Baoyu Zhao Wenming Liu Jun Zhao Li Li Tao Zhou 《Animal Feed Science and Technology》2008,142(1-2):74-88
Locoweeds cause significant livestock poisoning and economic loss all over the world. The purpose of this study was to evaluate the immune effect of locoweed toxin, swainsonine (SW), and human serum albumin (HSA) immunoconjugate (SW–HSA) on goats. Twenty four Sannon goats were randomly separated into immune control group, immune poisoning group I, immune poisoning group II and poisoning control group. Immune control group, immune poisoning group I and II were first vaccinated with SW–HSA immunoconjugate. Then, the poisoning control group, immune poisoning group I and II were fed with 10.0 g/kg BW/d dry powder of Oxytropis kansuensis Bunge in every morning and the duration of poisoning was 80 days. The immune control group was supplied with an alfalfa-based diet. Blood samples of these experimental animals were collected every 7 days after poisoning, and the serum enzymes α-mannosidase (AMA), lactate dehydrogenase (LDH), alkaline phosphatase (AKP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and blood urea nitrogen (BUN) were analyzed. Comparing with the poisoning control group, the changes of serum LDH, AKP, AST, AMA and BUN level of the two immune groups (I and II) were delayed 28, 14, 28, 21, 14 days, respectively, which indicated that SW–HSA immunoconjugate could prevent tissues containing BUN and these serum enzymes of the experimental animals from Oxytropis kansuensis Bunge injuring during these days. In the whole study, SW–HSA immunoconjugate and swainsonine did not influence serum ALT levels of the experimental goats. 相似文献