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Pradeep AR Jingade AH Singh CK Awasthi AK Kumar V Rao GC Prakash NB 《Genetics and molecular biology》2011,34(3):502-510
Deforestation and exploitation has led to the fragmentation of habitats and scattering of populations of the economically important eri silkworm, Samia cynthia ricini, in north-east India. Genetic analysis of 15 eri populations, using ISSR markers, showed 98% inter-population, and 23% to 58% intra-population polymorphism. Nei's genetic distance between populations increased significantly with altitude (R(2) = 0.71) and geographic distance (R(2) = 0.78). On the dendrogram, the lower and upper Assam populations were clustered separately, with intermediate grouping of those from Barpathar and Chuchuyimlang, consistent with geographical distribution. The Nei's gene diversity index was 0.350 in total populations and 0.121 in subpopulations. The genetic differentiation estimate (Gst) was 0.276 among scattered populations. Neutrality tests showed deviation of 118 loci from Hardy-Weinberg equilibrium. The number of loci that deviated from neutrality increased with altitude (R(2) = 0.63). Test of linkage disequilibrium showed greater contribution of variance among eri subpopulations to total variance. D('2)IS exceeded D('2)ST, showed significant contribution of random genetic drift to the increase in variance of disequilibrium in subpopulations. In the Lakhimpur population, the peripheral part was separated from the core by a genetic distance of 0.260. Patchy habitats promoted low genetic variability, high linkage disequilibrium and colonization by new subpopulations. Increased gene flow and habitat-area expansion are required to maintain higher genetic variability and conservation of the original S. c. ricini gene pool. 相似文献
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Pradeep AR Awasthi AK Singh CK Anuradha HJ Rao CG Vijayaprakash NB 《Journal of applied genetics》2011,52(3):345-353
Genetic structure of populations is under constant pressure from varying geographical conditions that induce phenotypic plasticity
in insects. Spatial distribution of 15 populations of Indian eri silkworm, Samia cynthia ricini originated at various altitudes of sub Himalayas based on Euclidean distance realized from yield attributes showed two population
clusters irrespective of their place of origin and altitude. However, DNA amplification profile by inter SSR (ISSR) markers
showed genetic variations among the populations depend on low and high altitudes. One ISSR locus each specific to high and
low altitude population was identified. The locus from high altitude showed deviation from Hardy-Weinberg equilibrium but
that from low altitude was in neutrality suggests that the high altitude loci could be under pressure from the altitudinal
variations. In association with different yield traits, 18 loci were identified. Of which, three markers showed association
with more than one trait indicative of pleiotropic influence. Stepwise addition of markers enhanced the correlation between
markers and the associated trait pointed to polygenic influence. Association of markers with altitude and yield traits suggests
an imperative relation of rare genetic loci with gene-environment interaction and phenotypic variability in S. c. ricini. 相似文献
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Pavankumar Jingade Soundararajan Sannasi Charu Sneha Jha 《Archives Of Phytopathology And Plant Protection》2018,51(3-4):128-138
Trichoderma are commonly used as bio control agents in various agro ecosystems. They are known to produce a variety of compounds that induce resistance responses in plants. Among different species of Trichoderma, T. harzianum, T. viride, T. koningii and T. hamatum are commercially used as bio control agents. In the present study, four commercially important species of Trichoderma isolated from coffee ecosystem were screened with sequence related amplified polymorphism (SRAP) markers. Among 48 SRAP primer pairs tested, 29 primers were polymorphic and generated 316 distinct scorable fragments. Out of 347 amplified fragments, 177 fragments were found polymorphic with an average of 6.10 fragments per primer combination. The average polymorphism information content (PIC) and resolving power (Rp) of the 29 polymorphic SRAP primer pair were 0.42 and 14.62, respectively. The UPGMA dendrogram clearly divided Trichoderma species into two broad clusters. The highest homology (83.0%) was observed between T. viride and T. Harzianum and the lowest homology (74.0%) was observed between T. Harzianum and T. konangii. Further, among 29 polymorphic SRAP markers screened, four primer pairs (ME1-EM3, ME1-EM20, ME1-EM22 and ME2-EM4) produced unique fragments specific to each species. These markers can be useful in easy and rapid identification of the species. 相似文献
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Bychappa Muniswamy Mishra Manoj Kumar Jingade Pavankumar Huded Arun K. C. 《Plant Cell, Tissue and Organ Culture》2019,139(1):91-103
Plant Cell, Tissue and Organ Culture (PCTOC) - In coffee, the micropropagation technique can be efficiently used in mass multiplication of superior F1 hybrids which is difficult using the... 相似文献
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