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Jie-Xiu Ouyang Tao Luo Hui-Yun Sun Jian Huang Dan-Feng Tang Lei Wu Yue-Hui Zheng Li-Ping Zheng 《Molecular and cellular biochemistry》2013,380(1-2):195-202
Pten (phosphatase and tensin homolog deleted on chromosome 10), a kind of tumor suppressor gene, plays important roles in female reproductive system. But its expression and roles in the formation of polycystic ovaries are yet to be known. In this study, we constructed a rat model of PCOS using norethindrone and HCG injections and found the expressions of pten mRNA and PTEN protein increased significantly in the polycystic ovary tissue by immunohistochemistry, RT-PCR, and western blot. Furthermore, the results showed that in vivo ovaries could be effectively transfected by lentiviral vectors through the ovarian microinjection method and indicated that pten shRNA may inhibit the formation of polycystic ovaries by pten down-regulation. Our study provides new information regarding the role of PTEN in female reproductive disorders, such as polycystic ovary syndrome. 相似文献
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目的:研究血红素代谢限速酶和珠蛋白代谢在运动性贫血发生机理中的功能和作用,及营养补充对运动性贫血防治效果的作用机制。方法:本实验对30只雄性Wistar大鼠进行等量随机分为3组(n=10):对照组(C)、运动组(P)和运动+营养组(G)。30m/min、0%坡度、每次1min为起始训练方式,前5周和后4周时训练时间的加速度为每次2min,训练频率为每天2次(前两周例外)。11周的跑台运动结束后应用RT-PCR和免疫组织化学的方法测试骨髓每氨基-γ酮戊酸合成酶(ALAs)、铁螯合酶(ferrochelatase)、α-珠蛋白、β-珠蛋白的基因表达和肝脏血红素氧舍酶-1(HO-1)的活性。结果:11周跑台运动可以增加大鼠肝脏HO-1的活性和骨髓β-珠蛋白的基因表达(P〈0.01,P〈0.05),抗运动性贫血复合剂补充并不能改变大鼠运动后血红素代谢限速酶和珠蛋白基因表达和活性,且运动+营养组大鼠肝脏HO-1活性水平显著高于对照组(P〈0.01),即递增负荷跑台运动不能影响大鼠骨髓血红素合成酶和α-珠蛋白的基因表达,但能够影响大鼠肝脏血红素分解酶的活性水平和骨髓β-珠蛋白的基因表达。结论:肝脏HO-1活性水平的升高可能是运动性贫血表现出低Hb、RBC和Hct水平的原因之一。 相似文献
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