全文获取类型
收费全文 | 132篇 |
免费 | 14篇 |
出版年
2020年 | 2篇 |
2016年 | 6篇 |
2015年 | 4篇 |
2014年 | 7篇 |
2013年 | 5篇 |
2012年 | 6篇 |
2011年 | 1篇 |
2010年 | 5篇 |
2009年 | 1篇 |
2008年 | 4篇 |
2007年 | 9篇 |
2006年 | 6篇 |
2005年 | 11篇 |
2004年 | 4篇 |
2003年 | 4篇 |
2002年 | 1篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1977年 | 3篇 |
1974年 | 4篇 |
1972年 | 1篇 |
1971年 | 4篇 |
1970年 | 1篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1958年 | 1篇 |
1930年 | 1篇 |
1929年 | 1篇 |
1922年 | 1篇 |
1914年 | 1篇 |
排序方式: 共有146条查询结果,搜索用时 203 毫秒
1.
2.
3.
Transport of l-aspartic acid in Neurospora crassa conidia is shown to be mediated by neutral and general amino acid transport systems. The transport activity is dependent on pH and results in accumulation of l-aspartic acid against a gradient. Mutants deficient in transport of l-aspartic acid are described. 相似文献
4.
5.
Despite the potential importance of adult nutrition in the ecology of parasitoids, information on the range of natural foods
they exploit is confined to a relatively small number of species. We provide some simple guidelines for determining dietary
range in adult parasitoids, both to facilitate and to encourage further research into this aspect of parasitoid biology.
相似文献
6.
The lactate dehydrogenase (LDH) from soya bean has been purified to homogeneity by affinity chromatography. The enzyme was purified by sequential adsor 相似文献
7.
8.
9.
Annemarie MM Vlaar Angela EP Bouwmans Marinus JPG van Kroonenburgh Werner H Mess Selma C Tromp Piet GWM Wuisman Alfons GH Kessels Ania Winogrodzka Wim EJ Weber 《BMC neurology》2007,7(1):28
Background
Parkinson's disease (PD) is the second most common neurodegenerative disorder. As there is no definitive diagnostic test, its diagnosis is based on clinical criteria. Recently transcranial duplex scanning (TCD) of the substantia nigra in the brainstem has been proposed as an instrument to diagnose PD. We and others have found that TCD scanning of substantia nigra duplex is a relatively accurate diagnostic instrument in patients with parkinsonian symptoms. However, all studies on TCD so far have involved well-defined, later-stage PD patients, which will obviously lead to an overestimate of the diagnostic accuracy of TCD. 相似文献10.
Jervis EJ Guarna MM Doheny JG Haynes CA Kilburn DG 《Biotechnology and bioengineering》2005,91(3):314-324
The extracellular matrix provides structural components that support the development of tissue morphology and the distribution of growth factors that modulate the overall cellular response to those growth factors. The ability to manipulate the presentation of factors in culture systems should provide an additional degree of control in regulating the stimulation of factor-dependent cells for tissue engineering applications. Cellulose binding domain (CBD) fusion protein technology facilitates the binding of bioactive cytokines to cellulose materials, and has permitted the analysis of several aspects of cell stimulation by surface-localized growth factors. We previously reported the synthesis and initial characterization of a fusion protein comprised of a CBD and murine stem cell factor (SCF) (Doheny et al. [1999] Biochem J 339:429-434). A significant advantage of the CBD fusion protein system is that it permits the stimulation of factor-dependent cells with localized growth factor, essentially free of nonfactor-derived interactions between the cell and matrix. In this work, the long-term stability and bioactivity of SCF-CBD fusions adsorbed to microcrystalline cellulose under cell culture conditions is demonstrated. Cellulose-bound SCF-CBD is shown to stimulate receptor polarization in the cell membrane and adherence to the cellulose matrix. In addition, cellulose-surface presentation of the SCF-CBD attenuates c-kit dephosphorylation kinetics, potentially modulating the overall response of the cell to the SCF signal. 相似文献