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Isolation and Characterization of Thermophilic Bacterial Strains with Inulinase Activity 总被引:6,自引:2,他引:4
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Jean-Jacques Allais Gladys Hoyos-Lopez Sadok Kammoun Jacques C. Baratti 《Applied microbiology》1987,53(5):942-945
Nine bacterial strains growing on inulin as the sole carbon and energy source were isolated from soil samples by enrichment culture on a mineral medium. Four of the strains were thermophilic and belong to the genus Bacillus. The thermophilic strains synthesized a β-fructosidase that was active on both inulin and sucrose. The presence of inulin in the culture medium is necessary for enzyme synthesis. Most of the activity on inulin was recovered in the culture medium, and the enzyme was synthesized during cell growth. 相似文献
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Human elongation factor 1α: a polymorphic and conserved multigene family with multiple chromosomal localizations 总被引:1,自引:0,他引:1
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P Cottin S Poussard D Mornet J J Brustis M Mohammadpour J Leger A Ducastaing 《Biochimie》1992,74(6):565-570
Dystrophin is a cytoskeletal protein which is thought to play an important role in membrane physiology since its absence (due to gene deficiency) leads to the symptoms of Duchenne muscular dystrophy (DMD). Some disruption in the regulation of intracellular free Ca2+ levels could lead to DMD-like symptoms. In this study, calpains, which are very active calcium-dependent proteases, were examined for their capacity to hydrolyse dystrophin in vitro. The results show that calpains are able to split dystrophin and produce breakdown products of different sizes (the degree of cleavage being dependent on the incubation time with proteases). The time-course of protease degradation was examined by Western immunoblot using three polyclonal sera which were characterized as being specific to the central (residues 1173-1728) and two distal parts of the molecule ie specific to the N-terminal (residues 43-760) or the C-terminal (residues 3357-3660) extremities of the dystrophin molecule. The cleavage patterns of dystrophin showed an accumulation of some major protease-resistant fragments of high relative molecular mass (250-370 kDa). These observations demonstrate that calpains digest dystrophin very rapidly when the calcium concentration is compatible with their activation. For instance, it is clear that calpains first give rise to large dystrophin products in which the C-terminal region is lacking. These observations suggest that dystrophin antibodies specific to the central domain of the molecule should be used to detect dystrophin for diagnostic purposes and before any conclusion as to the presence or absence of dystrophin can be deduced from results obtained using immunoanalyses of muscle biopsies. 相似文献
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Eveline De Mdicis Jean Paquette Jean-Jacques Gauthier Dennis Shapcott 《Applied microbiology》1986,52(3):567-573
Magnesium and manganese contents were measured by atomic absorption spectrophotometry in bacteria of several halophilic levels, in Vibrio costicola, a moderately halophilic eubacterium growing in 1 M NaCl, Halobacterium volcanii, a halophilic archaebacterium growing in 2.5 M NaCl, Halobacterium cutirubrum, an extremely halophilic archaebacterium growing in 4 M NaCl, and Escherichia coli, a nonhalophilic eubacterium growing in 0.17 M NaCl. Magnesium and manganese contents varied with the growth phase, being maximal at the early log phase. Magnesium and manganese molalities in cell water were shown to increase with the halophilic character of the logarithmically growing bacteria, from 30 mmol of Mg per kg of cell water and 0.37 mmol of Mn per kg of cell water for E. coli to 102 mmol of Mg per kg of cell water and 1.6 mmol of Mn per kg of cell water for H. cutirubrum. The intracellular concentrations of manganese were determined independently by a radioactive tracer technique in V. costicola and H. volcanii. The values obtained by 54Mn loading represented about 70% of the values obtained by atomic absorption. The increase of magnesium and manganese contents associated with the halophilic character of the bacteria suggests that manganese and magnesium play a role in haloadaptation. 相似文献
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Eduardo Agosin Jean-Jacques Daudin Etienne Odier 《Applied microbiology and biotechnology》1985,22(2):132-138
Summary 74 Basidiomycetes have been tested for ligninolytic capability on (14C)lignin-labelled wheat straw. Fifteen strains were selected and rested more accurately for ligninolytic activity and the capacity to degrade wheat straw. The asymptote, inflexion point and degradation rate were determined using a model approach. The fungi exhibited very different responses with respect to lignin biodegradation: high asymptote for Pleurotus ostreatus (77%), low inflexion points for Sporotrichum pulverulentum Nov. (6.1 days) and Pycnoporus spp. (2.7 to 4.7 days) with high and slow degradation rates, respectively (0.91% and 0.45% of 14CO2 release/day). Degradation values for (14C)whole-labelled wheat straw exhibited less variation. Finally, the strains Pleurotus ostreatus, Dichomitus squalens and Bjerkandera adusta showed the highest selectivity of lignin removal. 相似文献
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Jean-Jacques Fleury 《Geobios》1974,7(4):307-322
The formerly unknown microspheric form of Raadshoovenia salentina (Papetti and Tedeschi) and the new genus Cyclopseudedomia (type-species Cyclopseudedomia smouti n.g., n.sp.) are described from limestones of Campanian-Maastrichtian age from Gavrovo-Tripolitza zone in Greece. The new genus is distinguished from others of the sub-family RhapydionininaeKeijzer emend. Hamaoui and Fourcade by its almost entirely evolute test made of chambers tending to a cyclical stage (rapidly reached in the microspheric form). 相似文献
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