Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system

Environmental conditions likely affect physiology and behaviour of mice used for life sciences research on Earth or in Space. Here, we analysed the effects of cage confinement on the weightbearing musculoskeletal system, behaviour and stress of wild-type mice (C57BL/6JRj, 30 g b.wt., total n = 24) housed for 25 days in a prototypical ground-based and fully automated life support habitat device called “Mice in Space” (MIS). Compared with control housing (individually ventilated cages) the MIS mice revealed no significant changes in soleus muscle size and myofiber distribution (type I vs. II) and quality of bone (3-D microarchitecture and mineralisation of calvaria, spine and femur) determined by confocal and micro-computed tomography. Corticosterone metabolism measured non-invasively (faeces) monitored elevated adrenocortical activity at only start of the MIS cage confinement (day 1). Behavioural tests (i.e., grip strength, rotarod, L/D box, elevated plus-maze, open field, aggressiveness) performed subsequently revealed only minor changes in motor performance (MIS vs. controls). The MIS habitat will not, on its own, produce major effects that could confound interpretation of data induced by microgravity exposure during spaceflight. Our results may be even more helpful in developing multidisciplinary protocols with adequate scenarios addressing molecular to systems levels using mice of various genetic phenotypes in many laboratories. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Evidence of force exchanges during the six-legged walking of the bottom-dwelling fish, Chelidonichthys lucerna

Locomotion in terrestrial vertebrates is supposed to be derived from preadaptation in bottom-dwelling fish. A few fish species have been assumed to walk on the substratum, on the basis of coordinated movements of their paired fins. However, the validity of this assumption has remained uncertain, because of a lack of evidence that their fin rays actually exert a force on the substratum. Here, we provide the first conclusive evidence that a benthic teleost fish, the gurnard, Chelidonichthys lucerna (Triglidae), exerts forces on the substratum during its temporary bottom-dwelling hexapod locomotion. This demonstration was achieved by the use of a photoelastic gel technique combined with a force calibration device. The movement patterns of the three first pairs of rays of the pectoral fins were analysed in relation to the forces exerted on the substratum, by measuring deformations of the photoelastic gel substratum produced by the rays. The rays were shown to produce a force pattern that confirmed the existence of a hexapod locomotion in a vertebrate that was consistent with body propulsion and voluntary substratum walking.     

Cannabis and Fantasies of Development: Revaluing Relations through Land in Rural Papua New Guinea

Over the past decade, marijuana has become a significant element within Papua New Guinea's communities, revealing an important connection to the broader political economy. For young men, fluctuating commodity prices, the intermittent exploitation of mineral wealth and a reluctant tourist economy only gives them a taste for development. Marijuana seems to offer its permanence. Somewhere between the harsh reality of local economic and ecological futures young men near the town of Wau (Morobe Province) imagine themselves as successful entrepreneurs in the emerging drug trade. In particular, I consider how young men imagine the planting of this illicit crop as mediating tensions between acting individually and acting communally. While most have yet to take action on these fantasies, they provide insight into the development aspirations of rural Papua New Guineans. In this paper, I examine these development fantasies as they speak to a broader political economy and transformations of local landscapes throughout rural Pacific communities.     

The dynamics of Wood mouse (Apodemus sylvaticus) populations in the Camargue

The ecology of two monospecific populations of Wood mice ( Apodemus sylvaticus ) was studied using mark-recapture techniques in a high grassland and a low grassland of the Camargue. Annual changes in population size resembled the classical pattern of variation for European Wood mice. Numbers were low in summer, and peaks in numbers occurred in autumn and decreased sharply in early spring. However, the densities of the populations in winter were higher than many other European Wood mouse populations. The Wood mouse population in the high grassland area was dense and showed regular variations throughout the year. In the low grassland area, the population was lower and showed less regular variations, especially in winter, coinciding with soil flooding. Reproduction-related differences between the demographic parameters of the two sexes pointed to the use of differential space use strategies by males and females.     

Ontogeny of mouse vestibulo-ocular reflex following genetic or environmental alteration of gravity sensing

The vestibular organs consist of complementary sensors: the semicircular canals detect rotations while the otoliths detect linear accelerations, including the constant pull of gravity. Several fundamental questions remain on how the vestibular system would develop and/or adapt to prolonged changes in gravity such as during long-term space journey. How do vestibular reflexes develop if the appropriate assembly of otoliths and semi-circular canals is perturbed? The aim of present work was to evaluate the role of gravity sensing during ontogeny of the vestibular system. In otoconia-deficient mice (ied), gravity cannot be sensed and therefore maculo-ocular reflexes (MOR) were absent. While canals-related reflexes were present, the ied deficit also led to the abnormal spatial tuning of the horizontal angular canal-related VOR. To identify putative otolith-related critical periods, normal C57Bl/6J mice were subjected to 2G hypergravity by chronic centrifugation during different periods of development or adulthood (Adult-HG) and compared to non-centrifuged (control) C57Bl/6J mice. Mice exposed to hypergravity during development had completely normal vestibulo-ocular reflexes 6 months after end of centrifugation. Adult-HG mice all displayed major abnormalities in maculo-ocular reflexe one month after return to normal gravity. During the next 5 months, adaptation to normal gravity occurred in half of the individuals. In summary, genetic suppression of gravity sensing indicated that otolith-related signals might be necessary to ensure proper functioning of canal-related vestibular reflexes. On the other hand, exposure to hypergravity during development was not sufficient to modify durably motor behaviour. Hence, 2G centrifugation during development revealed no otolith-specific critical period.     

Photographing Spirits: Biangai Photography,Ancestors, and the Environment in Morobe Province,Papua New Guinea

In this article, I examine the intertwined meaning of photographs as image, object, and expression of the relationship created between photographer and subject among the Biangai, a people of Morobe Province, Papua New Guinea. These relations are highlighted through the method of indigenous photography, in which photographic authority is relinquished and the resulting images are discussed. It is argued that the camera becomes the lens through which the past interrogates the present, and the photograph as object and image circulates in networks for which these ancestral persons remain powerful and emotive.     

The Taste of Public Places: Terroir in Papua New Guinea's Emerging Nation

This paper considers the relationship between persons, land and food as seen in the sociality of public gatherings and gardening spaces. For Biangai speakers in the Upper Bulolo Valley of Morobe Province (Papua New Guinea), intimacies of place are often read through the work of tending to the land, the symbolic association of crops and people as well as rituals where male and female persons are made. By examining the intersection of taste and place in a public multi-language gathering, the paper offers a critical assessment of the French concept of le goût du terroir, or the taste of place, suggesting that tastes matter substantially in defining intergroup sociality. The paper reveals the significance of terroir for organising and strengthening group identity in Papua New Guinea, and its problematic extension to ideas of national foodviews. Ultimately, it is argued that such grounding informs how Biangai imagine the nation and its public places.