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Hermógenes Fernández-Marín David R. Nash Sarah Higginbotham Catalina Estrada Jelle S. van Zweden Patrizia d'Ettorre William T. Wcislo Jacobus J. Boomsma 《Proceedings. Biological sciences / The Royal Society》2015,282(1807)
Fungus-farming ant colonies vary four to five orders of magnitude in size. They employ compounds from actinomycete bacteria and exocrine glands as antimicrobial agents. Atta colonies have millions of ants and are particularly relevant for understanding hygienic strategies as they have abandoned their ancestors'' prime dependence on antibiotic-based biological control in favour of using metapleural gland (MG) chemical secretions. Atta MGs are unique in synthesizing large quantities of phenylacetic acid (PAA), a known but little investigated antimicrobial agent. We show that particularly the smallest workers greatly reduce germination rates of Escovopsis and Metarhizium spores after actively applying PAA to experimental infection targets in garden fragments and transferring the spores to the ants'' infrabuccal cavities. In vitro assays further indicated that Escovopsis strains isolated from evolutionarily derived leaf-cutting ants are less sensitive to PAA than strains from phylogenetically more basal fungus-farming ants, consistent with the dynamics of an evolutionary arms race between virulence and control for Escovopsis, but not Metarhizium. Atta ants form larger colonies with more extreme caste differentiation relative to other attines, in societies characterized by an almost complete absence of reproductive conflicts. We hypothesize that these changes are associated with unique evolutionary innovations in chemical pest management that appear robust against selection pressure for resistance by specialized mycopathogens. 相似文献
3.
We have reported previously that, when exposed to hypercapnia of various intensities, the diaphragm reduces its force of twitch and tetanic contractions in the in vitro rat preparation as well as in the in vivo dog preparation. The experiments reported here with 31P nuclear magnetic resonance (31P-NMR) spectroscopy attempt to examine cellular mechanisms that might be responsible for this deterioration in mechanical performance. Specifically they describe certain characteristics of this preparation and cautions needed to study the resting in vitro rat diaphragm with such techniques. Second, they report the response of intracellular pH (pHi), phosphocreatine (PCr), ATP, and inorganic phosphate (Pi) in the resting in vitro rat diaphragm exposed to long-term normocapnia or to long-term hypercapnia. The results show that 1) to maintain a viable preparation, it was necessary to keep the diaphragm extended to an area approximating that at functional residual capacity, 2) the diaphragm seemed quite capable of maintaining a constant pHi and constant contents of ATP and Pi during normocapnia, but there was a gradual decline in PCr, and 3) during hypercapnia there was a significant decrease in pHi, but the behavior of the phosphate metabolites was exactly as during normocapnia. The results suggest that the decrease in mechanical performance of the diaphragm is probably not due to a decrease in the availability of the high-energy phosphates, although they do not completely exclude this possibility or possibilities related to regional compartmentation. 相似文献
4.
Sauer Arne E.; Karg Gerhard; Koch Uwe T.; De Kramer Jacobus J.; Milli Roland 《Chemical senses》1992,17(5):543-553
A robust and portable apparatus for the measurement of pheromoneconcentrations under field conditions has been developed. Ituses the insect antenna (Lobesia botrana Hb.) itself as a sensitiveand specific pheromone detector. Shock-proof contact with theelectrodes is maintained by fixing the antenna in a specially-shapedplexiglass holder mounted within a glass tube. This allows measurementsto be made while moving the apparatus. Continuous airflow throughthe tube is generated by a suction pump and the incoming aircan be purified by passage through a charcoal filter. This allowsto readjust the offset and to calibrate the instrument by theapplication of pheromone pulses of known concentrations. Removalof the filter allows the direct access of ambient air over theantenna which responds by generating an electro-antennogram(EAG) as a measure of the pheromone concentration. Using the calibration curve, relative pheromone concentrationsin ambient air in a vineyard can be determined. Sample measurementsfrom areas treated with artificial pheromone for pest controlare presented. 相似文献
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In a controlled animal experiment the effects of dietary subacute Zn deficiency on growth, Zn concentration, and tissue 42-K
distribution were studied. Growth retardation caused lower body weight because both skeletal and heart muscle showed a reduction
in cell mass. Zn concentrations were reduced in most tissues, however, they remained unaltered in heart muscle. 42-K activity
increased in skeletal muscle and pancreas. We hypothesize the latter reflects the organs rate of metabolism, inducing the
exocrine pancreas to increase Zn absorption; in skeletal muscle it may induce also alterations in cell potentiation, causing
restless behavior. As suggested by the calculated specific K activity (Bq/mol), the K uptake was highest in liver and bone,
high in pancreas and skeletal muscle and low in heart muscle. The latter suggests K retention in heart muscle. Specific activity
in plasma and jejunum remained unaltered: K status and absorption seem unaffected. Zn deficiency causes different 42-K activities
in the various tissues, that respond by alterations in K metabolism without the induction of K deficiency. 相似文献
7.
Length growth, instar durations, fecundity and mortality rates of fivespecies of microcrustacean zooplankton from a tropical reservoir weremeasured in relation to food quantity and temperature in laboratorycultures. Three cladocerans (Ceriodaphnia cornuta, Moina micrura,Diaphanosoma excisum), one calanoid copepod (Heliodiaptomus viduus), and onecyclopoid copepod (Mesocyclops thermocyclopoides) were studied. Filteredseston (45 µm mesh) from a local pond was used for food. Two foodconcentrations were employed: (1) 10 µg chlorophyll l–1(ca 0.25 mg Cl–1), and (2) 50 µg chlorophylll–1 (ca 1.25 mg C l–1). Food levels and watertemperature (22.5, 27.5, and 32.5 °C) used, roughly covered the rangesfound in the reservoir. Although all the three growth parameters were oftenaffected to some degree by temperature and food, the quantitative responseof the species differed. Also, the species reacted differently to the threepossible interactions (i.e. food×temperature,food×instar, andtemperature×instar). This contributed to the overalldifferences in the species responses. For the cladocerans, instar durationswere always affected by temperature. The food did not affect the durationtime of the adults and that of the combined juvenile instars, the latterexcept in one case significantly. For the two copepods food level affectedthe duration times of naupliar and copepodite instars, but the effect oftemperature was only significant for H. viduus. The development timesobserved for H. viduus were extraordinary long compared with values reportedin the literature for other tropical calanoids. This suggests that foodconditions in our culture were adversely affecting its growth rates. 相似文献
8.
Mitochondrial DNA sequence evolution in sharks: rates, patterns, and phylogenetic inferences 总被引:8,自引:0,他引:8
Abundant representation of sharks in the fossil record makes this group a
superb system in which to investigate rates and patterns of molecular
evolution and to explore the strengths and weaknesses of phylogenetic
inferences from molecular data. In this report, the molecular evolution of
the cytochrome b gene in sharks is described and the information related to
results from phylogenetic analysis of the data evaluated in the light of a
phylogeny derived independently of the molecular data. Across divergent
lineages of sharks there is evidence for significant substitution rate
variation, departure from compositional equilibrium, and substantial
homoplasy; nevertheless, the signal of evolutionary history is evident in
patterns of shared transversions and amino acid replacements.
相似文献
9.
There is marked heterogeneity of nucleotide composition in mitochondrial
DNA across divergent animals. Differences in nucleotide composition
presumably reflect differences in directional nucleotide substitution for
A+T or G+C nucleotides. In mitochondrial DNA, there is A+T directional
nucleotide substitution in most (if not all) animals surveyed, and the
magnitude of directional A+T nucleotide substitution differs greatly within
and among groups. Differences in directional nucleotide substitution among
lineages of mammals can be explained by changes in metabolic physiology.
This relationship is thought to be mediated by the effect of oxygen
radicals because these toxic compounds are by-products of aerobic
metabolism and are known mutagens. Association between metabolism and
nucleotide composition provides additional evidence in favor of the
hypothesis that rates and patterns of nucleotide substitution in
mitochondrial DNA can be influenced by factors that impinge on rates of
endogenous DNA damage.
相似文献
10.
Elemental distribution in striated muscle and the effects of hypertonicity: Electron probe analysis of cryo sections 总被引:15,自引:4,他引:11
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A method of rapid freezing in supercooled Freon 22 (monochlorodifluoromethane) followed by cryoultramicrotomy is described and shown to yield ultrathin sections in which both the cellular ultrastructure and the distribution of diffusible ions across the cell membrane are preserved and intracellular compartmentalization of diffusabler ions can be quantitated. Quantitative electron probe analysis (Shuman, H., A.V. Somlyo, and A.P. Somlyo. 1976. Ultramicros. 1:317-339.) of freeze-dried ultrathin cryto sections was found to provide a valid measure of the composition of cells and cellular organelles and was used to determine the ionic composition of the in situ terminal cisternae of the sarcoplasmic reticulum (SR), the distribution of CI in skeletal muscle, and the effects of hypertonic solutions on the subcellular composition if striated muscle. There was no evidence of sequestered CI in the terminal cisternae of resting muscles, although calcium (66mmol/kg dry wt +/- 4.6 SE) was detected. The values of [C1](i) determined with small (50-100 nm) diameter probes over cytoplasm excluding organelles over nuclei or terminal cisternae were not significantly different. Mitochondria partially excluded C1, with a cytoplasmic/ mitochondrial Ci ratio of 2.4 +/- 0.88 SD. The elemental concentrations (mmol/kg dry wt +/- SD) of muscle fibers measured with 0.5-9-μm diameter electron probes in normal frog striated muscle were: P, 302 +/- 4.3; S, 189 +/- 2.9;C1, 24 +/- 1.1;K, 404 +/- 4.3, and Mg, 39 +/- 2.1. It is concluded that: (a) in normal muscle the "excess CI" measured with previous bulk chemical analyses and flux studies is not compartmentalized in the SR or in other cellular organelles, and (b) the cytoplasmic C1 in low [K](0) solutions exceeds that predicted by a passive electrochemical distribution. Hypertonic 2.2 X NaCl, 2.5 X sucrose, or 2.2 X Na isethionate produced: (a) swollen vacuoles, frequently paired, adjacent to the Z lines and containing significantly higher than cytoplasmic concentrations of Na and Cl or S (isethionate), but no detectable Ca, and (b) granules of Ca, Mg, and P = approximately (6 Ca + 1 Mg)/6P in the longitudinal SR. It is concluded that hypertonicity produces compartmentalized domains of extracellular solutes within the muscle fibers and translocates Ca into the longitudinal tubules. 相似文献