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1.
The role of DNA sequence in determining nucleosome positions in vivo was investigated by comparing the positions adopted by nucleosomes reconstituted on a yeast plasmid in vitro using purified core histones with those in native chromatin containing the same DNA, described previously. Nucleosomes were reconstituted on a 2.5 kilobase pair DNA sequence containing the yeast TRP1ARS1 plasmid with CUP1 as an insert (TAC-DNA). Multiple, alternative, overlapping nucleosome positions were mapped on TAC-DNA. For the 58 positioned nucleosomes identified, the relative positioning strengths and the stabilities to salt and temperature were determined. These positions were, with a few exceptions, identical to those observed in native, remodeled TAC chromatin containing an activated CUP1 gene. Only some of these positions are utilized in native, unremodeled chromatin. These observations suggest that DNA sequence is likely to play a very important role in positioning nucleosomes in vivo. We suggest that events occurring in yeast CUP1 chromatin determine which positions are occupied in vivo and when they are occupied. 相似文献
2.
HIF-1 expression in healing wounds: HIF-1alpha induction in primary inflammatory cells by TNF-alpha 总被引:12,自引:0,他引:12
3.
Patterns of Sleeping Site and Sleeping Tree Selection by Black-and-Gold Howler Monkeys (Alouatta caraya) in Northern Argentina 总被引:1,自引:0,他引:1
Brividoro Melina V. Kowalewski Martin M. Scarry Clara J. Oklander Luciana I. 《International journal of primatology》2019,40(3):374-392
International Journal of Primatology - The selection of sleeping sites and sleeping trees in nonhuman primates is related to social and ecological factors. We investigate the role of body... 相似文献
4.
ABSTRACT The phrase persistent vulnerability reflects the enduring relationship of the rural population in Ceará with a highly variable climate. Persistence underscores the historical and unyielding nature of this vulnerability. Yet contrary to once-catastrophic rates of mortality etched in a public consciousness, no one dies from severe droughts and few people flee them as in the past. Government relief and social transfers have become the institutionalized form of adaptation, giving way to the counterintuitive reality that drought stabilizes the food and income supply for poor people. We analyze how maladaptive risk reduction, which is embedded in clientilistic social relations, undermines resilience, and we examine pathways toward a more sustainable adaptive relationship. 相似文献
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Monitoring signal transduction in cancer: cDNA microarray for semiquantitative analysis. 总被引:1,自引:0,他引:1
H B Hsieh R A Lersch D E Callahan S Hayward M Wong O H Clark H U Weier 《The journal of histochemistry and cytochemistry》2001,49(8):1057-1058
This study targeted the development of a novel microarray tool to allow rapid determination of the expression levels of 58 different tyrosine kinase (tk) genes in small tumor samples. The goals were to define a reference probe for multi-sample comparison and to investigate the variability and reproducibility of the image acquisition and RT-PCR procedures. The small number of tk genes on our arrays enabled us to define a reference probe by artificially mixing all genes on the arrays. Such a probe provided contrast reference for comparative hybridization of control and sample DNA and enabled cross-comparison of more than two samples against one another. Comparison of signals generated from multiple scanning eliminated the concern of photo bleaching and scanner intrinsic noise. Tests performed with breast, thyroid, and prostate cancer samples yielded distinctive patterns and suggest the feasibility of our approach. Repeated experiments indicated reproducibility of such arrays. Up- or downregulated genes identified by this rapid screening are now being investigated with techniques such as in situ hybridization. 相似文献
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Bugreev D. V. Sinitsyna O. I. Buneva V. N. Nevinsky G. A. 《Russian Journal of Bioorganic Chemistry》2003,29(3):249-261
Data on the interaction of DNA type I topoisomerases from the murine and human placenta cells with specific and nonspecific oligonucleotides of various structures and lengths are summarized. The relative contributions of various contacts between the enzymes and DNA that have previously been detected by X-ray analysis to the total affinity of the topoisomerases for DNA substrates are estimated. Factors that determine the differences in the enzyme interactions with specific and nonspecific single- and double-stranded DNAs are revealed. The results of the X-ray analysis of human DNA topoisomerase I are interpreted taking into account data on the comprehensive thermodynamic and kinetic analysis of the enzyme interaction with the specific and nonspecific DNAs. 相似文献
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