Further studies on the occurrence of freshwater Microturbellaria in the British Isles

Five new British records of freshwater Microturbellaria are illustrated: Gieysztoria infundibuliformis, Castrada lanceola, Castrada neocomensis, Castrada viridis and Strongylostoma elongatum. These species together with a new species, Macrostomum johni described by Young (1972), are woven into the fabric of the existing key for the group (Young, 1970). All six species were recorded from the littoral zone of lakes in Caernarvonshire, N. Wales. The times of the recordings are indicated.     

Principles of diagnosing occupational diseases

The traditional diagnostic procedure adhered to in general medicine is too frequently neglected when a physician is confronted with a possible occupational disease. Also, the proper diagnosis in occupational medicine requires additional effort along lines of investigation not necessary in general medicine. Failure to conform to the basic steps as outlined in this paper will distort known entities or "create" false ones.     

The effect of changes of environment on the electrical and ionic pattern of muscle

The resting and action potentials of sartorius muscles of the toad, Bufo marinus, have been measured under varying conditions of external environment. At the same time, analyses for Na(+) and K(+) content were carried out. There was a slight elevation of 2 mv. when the measurements were made in phosphate-Ringer instead of in bicarbonate-Ringer. The R.P. was independent of the hydrogen ion concentration between pH 6.5 and 8.5, although at these pH's there was marked alteration in the level of Na(+) and K(+) in the muscle. Alteration of the external K(+) level between 0 and 50 m.eq./liter has little influence on the internal K(+) concentration. When the log of the external K(+) concentration is plotted against the R.P. there is not a linear relationship until the external K(+) is raised above 12 m.eq./liter, at which point the cell is unexcitable. Above this value a straight line with a slope of 58 mv. per ten-fold change in concentration is obtained, but the absolute values at any point are about 35 per cent higher than those which would be given by the Nernst equation. Alteration of the external Na(+) level within a range of 45 to 650 m.eq./liter resulted in marked changes in the internal Na(+) content, without, however, having any effect on the ratio Na(+) (out)/Na(+) (in). This ratio has remained at about 3 in spite of marked fluctuations in the absolute value of the internal and external Na(+) levels. When the Na(+) level is lowered there is a decrease in the height of the action potential although there is no alteration in the ratio Na(+) (out)/Na(+) (in). As the Na(+) level is raised the height of the action potential is not affected even in the presence of a fivefold increase in Na(+) in the Ringer. The results do not support the conclusion that the bioelectric potentials can be calculated from the ionic ratios by means of simple physical chemical hypotheses such as the Nernst or Goldman equations. The maintenance of the normal K(+) content of the cell cannot be accounted for by a Donnan mechanism. No definite evidence has been produced to explain the mechanism of a Na(+) "pump." In other words, the concept of a Na(+) pump requires that there shall be a physico- or organochemical mechanism which will distinguish between Na(+) and K(+) (or other) ions. There is evidence that Na(+) can be extruded against a concentration gradient. On the other hand the cell is able to maintain a constant ratio of external to internal Na(+) even when the cell has been severely damaged by very high external Na(+) levels.     

The effect of time of sowing on the incidence of subterranean clover red leaf virus infection in broad bean (Vicia faba)

Yield losses in broad beans due to subterranean clover red leaf virus (SCRLV) in 1972/73 were 21, 30, 61 and 8% in plots sown in May, July, September and November respectively. The variations in yield loss resulted from differing levels of virus infection during periods in which harvestable pods were set because further pod set virtually ceased after symptoms of infection became apparent. The increases in infection were paralleled by increases in the infestation of Aulacorthum solani the aphid vector of SCRLV. Yield losses were greatest in the September-sown plots because the plants emerged at the commencement of the spring peak of aphid flight and were least in the November-sown plots which emerged after the peak of aphid flight had declined. However, potential yields decreased with deferment of sowing time and recorded yields were greatest from May-sown plots.
Yield losses due to SCRLV in 1973/74 were 79 and 91% for plots sown in May and September respectively. These larger yield losses resulted from an earlier and more rapid colonisation of plants by A. solani than in the previous year.
Choice of May as sowing date would not have controlled the disease satisfactorily in 1973/74 but would have been practical in the previous season when colonisation by A. solani was delayed so that little infection with SCRLV occurred before pod set had ceased.     

THE CONTROL OF SEXUAL MORPHOGENESIS IN THE ASCOMYCOTINA

(1) A series of factors controls sexual morphogenesis in the Ascomycotina, a process involving the formation of novel structures such as ascocarps (fruit bodies) and asci (sacs containing spores) during sexual reproduction. (2) Environmental and genetic factors must be correct before Ascomycetes may sexually reproduce. Compatibility in many heterothallic species is under polygenic control, with the mating type loci and also other genetic factors determining the productivity of sexual crosses. (3) Classical genetic studies have shown that sexual morphogenesis involves the expression of a series of developmentally regulated genes, and this has been confirmed by recent molecular studies which have demonstrated changes in patterns of mRNA and protein synthesis during ascocarp formation. (4) Hyphal differentiation leading to the formation of mature fruit bodies occurs in response to a series of signals, which include various physical and chemical factors. (5) Chemical sex factors have been identified which are believed to have important regulatory or nutritional roles in sexual morphogenesis. These include the following. (a) Diffusible sex hormones which may regulate developmental switching between asexual and sexual modes of reproduction, including (i) pheromones involved with the induction of gametangia and gamete attraction, and (ii) sex morphogens involved with triggering particular stages of fruit body formation. (b) Sexual growth substances which are required as nutrients, and may be precursors for the production of sex hormones, or metabolites used in the synthesis of novel sexual structures. Most of these sex factors are lipids. (6) Certain sex morphogens and sexual growth substances have been shown to exhibit activity in a variety of fungal species, suggesting that fungi of related phylogenetic descent may utilize similar metabolites or signalling factors during sexual reproduction. (7) Phenoloxidase enzymes may catalyse hyphal aggregation in developing fruit bodies. (8) Initial stages of ascocarp development may occur independently of the events of the sexual cycle. However, a link(s) with the functional ascogenous hyphae is needed for the formation of morphologically mature ascocarps. (9) Suitable environmental conditions are sufficient to trigger sexual morphogenesis in homothallic Ascomycetes. However, an extra level of control is present in heterothallic species, with a compatible partner required to complete sexual reproduction. This may be partly because novel regulatory products, formed by the combined action of the mating type loci of different partners, are required for further ascocarp development. (10) Further research is required to identify more fungal chemical sex factors and to determine the role of environmental stress in controlling sexual morphogenesis, and how this may be related to temporal patterns in the expression of mating type genes.     

Plasminogen activator and collagenase production by cultured capillary endothelial cells

Cultured bovine capillary endothelial (BCE) cells produce low levels of collagenolytic activity and significant amounts of the serine protease plasminogen activator (PA). When grown in the presence of nanomolar quantities of the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA), BCE cells produced 5-15 times more collagenolytic activity and 2-10 times more PA than untreated cells. The enhanced production of these enzymes was dependent on the dose of TPA used, with maximal response at 10(-7) to 10(-8) M. Phorbol didecanoate (PDD), an analog of TPA which is an active tumor promoter, also increased protease production. 4-O-methyl-TPA and 4α-PDD, two analogs of TPA which are inactive as tumor promoters, had no effect on protease production. Increased PA and collagenase activities were detected within 7.5 and 19 h, respectively, after the addition of TPA. The TPA-stimulated BCE cells synthesized a urokinase-type PA and a typical vertebrate collagenase. BCE cells were compared with bovine aortic endothelial (BAE) cells and bovine embryonic skin (BES) fibroblasts with respect to their production of protease in response to TPA. Under normal growth conditions, low levels of collagenolyic activity were detected in the culture fluids from BCE, BAE, and BES cells. BCE cells produced 5-13 times the basal levels of collagenolytic activity in response to TPA, whereas BAE cells and BES fibroblasts showed a minimal response to TPA. Both BCE and BAE cells exhibited relatively high basal levels of PA, the production of which was stimulated approximately threefold by the addition of TPA. The observation that BCE cells and not BAE cells produced high levels of both PA and collagenase activities in response to TPA demonstrates a significant difference between these two types of endothelial cells and suggests that the enhanced detectable activities are a property unique to bovine capillary and microvessel and endothelial cells.     

Further studies on the occurrence of freshwater Microturbellaria in the British Isles

Macrostomwn johni sp. nov. from Llyn Cwellyn, N. Wales is described. The species has been recorded from the vegetational littoral in December and February only. The main distinguishing criterion is the penis stylet which consists of a funnel, about 80-98 μm in length in slightly squashed specimens, curved in two planes and with a sub-terminal distal opening, the distal end is blunt and cowl (hood) shaped. Other features include the presence of sensory hairs and rhabdoids all over the body, ‘Haft-papillen’, and eyes. Rhammite tracks are weakly developed. The testes are elongate and the ovaries are compact, though slightly indented, and smaller than the testes. A very short ductus intervesicularis is present. The vagina is short. A ‘Verschlussap-parat’ is present but poorly developed.