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1.
B Lymphocyte Colony Formation in vitro: Ultrastructural Development of Individual Colonies 总被引:1,自引:0,他引:1
POUL BRIX POULSEN MOGENS H. CLAËSSON 《Differentiation; research in biological diversity》1980,17(1-3):77-84
B lymphocyte colony development in agar culture was studied using an electron microscope, and more than 3,000 colony cells were identified and photographed. In early cultures (day 4) lymphoblasts dominated the colonies. From day 5 onwards plasmablasts and small lymphocytes were present in the colonies. From day 6 onward mature plasma cells were observed in increasing numbers. On day 9 of culture the colonies started to degenerate and on day 10 of culture approximately 70% of the colony consisted of pyknotic and degenerating cells. Topographically, the degenerating cells were concentrated in the center of the colony whereas proliferation took place in the periphery. Colony growth occurred in an exponential fashion, the number of viable colony cells being maximal on day 8 of culture (400–600 cells/colony). At this time the frequencies of the four B cell categories were: lymphoblasts 72%, plasmablasts 20%, plasma cells 6%, and small lymphocytes 2%. Recloning experiments showed that dispersed colony cells were capable of forming only small cell clusters. It is concluded that B lymphocyte colony formation reflects a series of B cell developmental stages including the formation of the end cell categories of this lymphocyte lineage. 相似文献
2.
SUMMARY.
- 1 Biomass, production and life history of Mysis relicta were studied in the large Lake Mjøsa during the years 1976–80. Biomass fluctuations were large, but biomass averaged about 1 g wet wt m?2 or 200 ind. m?2. Cumulative net production during summer ranged from 1.6 to 2.1 g wet wt m?2. Mean population P/B-ratio was close to 2.
- 2 Neither production or biomass of Mysis were correlated with the biomass of phytoplankton or the main Zooplankton prey species (Daphnia and Bosmina), suggesting a predominant ‘top down’ control of Mysis in this lake.
- 3 The life cycle was well synchronized with development of food items, but predation pressure also affected life-history patterns. The proportion of juveniles apparently increased in years with strong fish predation pressure, producing a predominantly 1-year life-cycle pattern the following year.
- 4 Mysis had a predominant 2-year life history, but a traction of the population may reproduce after 1 or 3 years. Reproduction occurred exclusively during winter. Even in such a large and stable system, where Mysis has coexisted with its main food items and predators for 8–10 000 years, a flexible life history is maintained and is probably an important buffer against year-to-year fluctuations in food and predator abundance.
3.
TORBEN MOTH IVERSEN JENS THORUP KARINA KJELDSEN NIELS THYSSEN 《Freshwater Biology》1991,26(2):189-198
SUMMARY. 1. Population growth rates and relative competitive abilities of the rotifer Keratella cochlearis f. tecta and the small-bodied cladoceran Daphnia ambigua were studied under different schedules of food addition but equal total food quantity (per 4-day interval). The initial population growth rate of Keratella was significantly affected by the feeding schedule and by the presence of competitors, while that of Daphnia was affected by neither factor. Population densities of both species tended to increase as the frequency of food addition increased.
2. Daphnia suppressed and excluded Keratella from mixed-species cultures when food was provided intermittently at a high concentration, but it failed to exclude the rotifer when food was provided in a near-continuous supply at low concentration. Keratella had only a minor suppressive effect on Daphnia in all mixed-species treatments.
3. Starvation experiments indicate that Daphnia is able to withstand food shortages for significantly longer periods of time than Keratella . These and other results indicate that the outcome of interspecific competition between these species may be influenced by me frequency and concentration at which food is supplied. Daphnia ambigua is competitively superior to K. cochlearis when food is concentrated or 'pulsed', but much less so when ambient food levels are chronically low. Patterns of food availability may have important effects in determining the relative abundance of rotifers and small cladocerans in natural zooplankton communities. 相似文献
2. Daphnia suppressed and excluded Keratella from mixed-species cultures when food was provided intermittently at a high concentration, but it failed to exclude the rotifer when food was provided in a near-continuous supply at low concentration. Keratella had only a minor suppressive effect on Daphnia in all mixed-species treatments.
3. Starvation experiments indicate that Daphnia is able to withstand food shortages for significantly longer periods of time than Keratella . These and other results indicate that the outcome of interspecific competition between these species may be influenced by me frequency and concentration at which food is supplied. Daphnia ambigua is competitively superior to K. cochlearis when food is concentrated or 'pulsed', but much less so when ambient food levels are chronically low. Patterns of food availability may have important effects in determining the relative abundance of rotifers and small cladocerans in natural zooplankton communities. 相似文献
4.
ERIK JEPPESEN ESBEN AGERBO MADSEN JENS PEDER JENSEN & N. ANDERSON 《Freshwater Biology》1996,36(1):115-127
1. As quantitative information on historical changes in fish community structure is difficult to obtain directly from fish remains in lake sediments, transfer function for planktivorous fish abundance has been developed based on zooplankton remains in surface sediment (upper 1 cm). The transfer function was derived using weighted average regression and calibration against contemporary data on planktivorous fish catch per unit effort (PF-CPUE) in multiple mesh size gill nets. Zooplankton remains were chosen because zooplankton community structure in lakes is highly sensitive to changes in fish predation pressure. The calibration data set consisted of thirty lakes differing in PF-CPUE (range 18–369 fish net–1), epilimnion total phosphorus (range 0.025–1.28 mg P l–1) and submerged macrophyte coverage (0–57%). 2. Correlation of log-transformed PF-CPUE, total phosphorus and submerged macrophyte coverage v the percentage abundance in the sediment of the dominant cladocerans and rotifers revealed that the typical pelagic species correlated most highly to PF-CPUE, while the littoral species correlated most highly to submerged macrophyte coverage. Consequently, only pelagic species were taken into consideration when establishing the fish transfer function. 3. Canonical correspondence analysis (CCA) revealed that the pelagic zooplankton assemblage was highly significantly related to PF-CPUE (axis 1), whereas the influence of total phosphorus and submerged macrophyte coverage was insignificant. Predicted PF-CPUE based on weighted average regression without (WA) and with (WA(tol)) downweighting of zooplankton species tolerance correlated significantly with the observed values (r2 = 0.64 and 0.60 and RMSE = 0.54 and 0.56, respectively). A marginally better relationship (r2 = 0.67) was obtained using WA maximum likelihood estimated optima and tolerance. 4. It is now possible, quantitatively, to reconstruct the historical development in planktivorous fish abundance based on zooplankton fossil records. As good relationships exist between contemporary PF-CPUE data and indicators such as the zooplankton/phytoplankton biomass ratio, Secchi depth and the maximum depth distribution of submerged macrophytes, it is now also possible to derive information on past changes in lake water quality and trophic structure. It will probably prove possible further to improve the transfer function by including other invertebrate remains, e.g. chironomids, Chaoborus, snails, etc., and its scope could be widened by including deeper lakes, more oligotrophic lakes, more acidic lakes and lakes with extensive submerged macrophyte coverage (in the latter case to enable use of the information in the fossil record on plant-associated cladocerans). 相似文献
5.
JENS MOGENS OLESEN 《Botanical journal of the Linnean Society. Linnean Society of London》1994,115(2):95-113
The forest herb Corydalis cava is the only known species with a corm where tissue is added annually as a layer on the outside, and where the older central parts rot away, i.e. the corm becomes an expanding sphere. This growth pattern creates energetic problems for ageing plants. However, older corms change their shape in a way that saves biomass. Growth of corm with age was here modelled introducing different corm traits saving mass and thus postponing death. The largest corm in the study patch had a volume of 60 ml. If such a corm had remained a solid sphere, its age would be estimated to be 8.3 years. In reality, 4 year old corms became hollow and later an opening appeared in their basal part. In addition, corms ≥ 7 years became more conical. A conical corm with a volume of 60 ml was estimated to be 15.9 years old. Volume and shape of corm thus made it possible to age individual plants. The age structure of the plants in the study patch was described and discussed in relation to the overall dynamics of the metapopulation. The patch consisted of a few strong age classes (from 5 to 7 years old). Any recruiting juvenile age classes were almost completely missing. 相似文献
6.
The role of the cilia in the locomotion (“gliding”) of Tetrahymena thermophila in a semi-solid medium has been studied when cells were migrating in gradients of attractant. Video recordings and computer-aided motion analysis of migrating cells and their ciliary activity show that Tetrahymena thermophila migrate by swimming forward in semi-solid methyl cellulose, using their cilia. Ciliary reversals occur at certain intervals and cause a termination (“stop”) of cellular migration. Cells with reversed cilia resume forward migration when normal ciliary beating resumes. In gradients of attractants, cells migrating towards the attractant suppress ciliary reversals, which leads to longer runs between stops than in control cells. Cells migrating away from the attractant have a higher frequency of ciliary reversals than the control cells resulting in shorter runs. Stimulated cells adapt to a particular ambient concentration of attractant several times during migration in the gradient. Adaptation is followed by de-adaptation, which occurs during the “stop”. In the presence of cycloheximide, a strong inhibitor of chemoattraction, the attractant-induced suppression of ciliary reversal is abolished (cells become desensitized to the attractant). It is concluded that Tetrahymena has a short-term memory during adaptation. This is important for the efficiency of migration towards an attractant. 相似文献
7.
8.
9.
Impact of submerged macrophytes on fish-zooplanl phytoplankton interactions: large-scale enclosure experiments in a shallow eutrophic lake 总被引:1,自引:0,他引:1
PER SCHRIVER JENS BØGESTRAND ERIK JEPPESEN MARTIN SøNDERGAARD 《Freshwater Biology》1995,33(2):255-270
1. The impact of changes in submerged macrophyte abundance on fish-zooplankton-phytoplankton interactions was studied in eighteen large-scale (100 m2) enclosures in a shallow eutrophic take. The submerged macrophytes comprised Potamategon pectinatus L., P. pusillus L. and Callitriche hermaphroditica L. while the fish fry stock comprised three-spined sticklebacks, Gasterosteus acuteatus L., and roach, Rutilus rutilus L. 2. In the absence of macrophytes zooplankton biomass was low and dominated by cyclopoid copepods regardless of fish density, while the phytoplankton biovolume was high (up to 38 mm31) and dominated by small pennate diatoms and chlorococcales. When the lake volume infested by submerged macrophytes (PVI) exceeded 15–20% and the fish density was below a catch per unit effort (CPUE) of 10 (approx. 2 fry m?2), planktonic cladoceran biomass was high and dominated by relatively large-sized specimens, while the phytoplankton biovolume was low and dominated by small fast-growing flagellates. At higher fish densities, zooplankton biomass and average biomass of cladocerans decreased and a shift to cyclopoids occurred, while phytoplankton biovolume increased markedly and became dominated by cyanophytes and dinoflagellates. 3. Stepwise multiple linear regressions on log-transformed data revealed that the biomass of Daphnia, Bosmina, Ceriodaphmia and Chydorus were all significantly positively related to PVI and negatively to the abundance of fish or PVI x fish. The average individual biomass of cladocerans was negatively related to fish, but unrelated to PVI. Calculated zooplankton grazing pressure on phytoplankton was positively related to PVI and negatively to PVI x fish. Accordingly the phytoplankton biovolume was negatively related to PVI and to PVI x zooplankton biomass. Cyanophytes and chryptophytes (% of biomass) were positively and Chlorococcales and diatoms negatively related to PVI, while cyanophytes and Chlorococcales were negatively related to PVI x zooplankton biomass. In contrast diatoms and cryptophytes were positively related to the zooplankton biomass or PVI x zooplankton. 4. The results suggest that fish predation has less impact on the zooplankton community in the more structured environment of macrophyte beds, particularly when the PVI exceeds 15–20%. They further suggest that the refuge capacity of macrophytes decreases markedly with increasing fish density (in our study above approximately 10 CPUE). Provided that the density of planktivorous fish is not high, even small improvements in submerged macrophyte abundance may have a substantial positive impact on the zooplankton, leading to a lower phytoplankton biovolume and higher water transparency. However, at high fish densities the refuge effect seems low and no major zooplankton mediated effects of enhanced growth of macrophytes are to be expected. 相似文献
10.
HENRIK GLENNER MARKJ. GRYGIER JENS T. HØEG PETER G.JENSEN FREDERICK R. SCHRAM F.L.S. 《Zoological Journal of the Linnean Society》1995,114(4):365-404
We present a cladistic analysis of the Cirripedia Thoracica using morphological characters and the Acrothoracica and Ascothoracida as outgroups. The list of characters comprised 32 shell and soft body features. The operational taxonomic units (OTUs) comprised 26 well-studied fossil and extant taxa, principally genera, since uncertainty about monophyly exists for most higher ranking taxonomic units. Parsimony analyses using PAUP 3.1.1 and Hennig86 produced 189 trees of assured minimal length. We also examined character evolution in the consensus trees using MacClade and Clados. The monophyly of the Balanomorpha and the Verrucomorpha sensu stricto is confirmed, and all trees featured a sister group relationship between the ‘living fossil Neoverruca and me Brachylepadomorpha. In the consensus trees the sequential progression of ‘pedunculate‘sister groups up to a node containing Neolepas also conforms to current views, but certain well-established taxa based solely on plesiomorphies stand out as paraphyletic, such as Pedunculata (= Lepadomorpha); Eolepadinae, Scalpellomorpha and Chthamaloidea. The 189 trees differed principally in the position of shell-less pedunculates, Neoverruca, the scalpelloid Capitulum, and the interrelationships within the Balanomorpha, although the 50% majority rule consensus tree almost fully resolved the latter. A monophyletic Sessilia comprising both Verrucomorpha and Balanomorpha appeared among the shortest trees, but not in the consensus. A tree with a monophyletic Verrucomorpha including Neoverruca had a tree length two steps longer than the consensus trees. Deletion of all extinct OTUs produced a radically different tree, which highlights the importance of fossils in estimating cirripede phylogeny. Mapping of our character set onto a manually constructed cladogram reflecting die most recent scenario of cirripede evolution resulted in a tree length five steps longer than any of our shortest trees. Our analysis reveals that several key questions in cirripede phylogeny remain unsolved, notably the position of shell-less forms and the transition from ‘pedunculate‘to ‘sessile‘barnacles. The inclusion of more fossil species at this point in our understanding of cirripede phylogeny will only result in even greater levels of uncertainty. When constructing the character list we also identified numerous uncertainties in the homology of traits commonly used in discussing cirripede evolution. Our study highlights larval ultrastructure, detailed studies of early ontogeny, and molecular data as the most promising areas for future research. 相似文献