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Mohd Zaki Salleh Lay Kek Teh Lian Shien Lee Rose Iszati Ismet Ashok Patowary Kandarp Joshi Ayesha Pasha Azni Zain Ahmed Roziah Mohd Janor Ahmad Sazali Hamzah Aishah Adam Khalid Yusoff Boon Peng Hoh Fazleen Haslinda Mohd Hatta Mohamad Izwan Ismail Vinod Scaria Sridhar Sivasubbu 《PloS one》2013,8(8)
Background
With a higher throughput and lower cost in sequencing, second generation sequencing technology has immense potential for translation into clinical practice and in the realization of pharmacogenomics based patient care. The systematic analysis of whole genome sequences to assess patient to patient variability in pharmacokinetics and pharmacodynamics responses towards drugs would be the next step in future medicine in line with the vision of personalizing medicine.Methods
Genomic DNA obtained from a 55 years old, self-declared healthy, anonymous male of Malay descent was sequenced. The subject''s mother died of lung cancer and the father had a history of schizophrenia and deceased at the age of 65 years old. A systematic, intuitive computational workflow/pipeline integrating custom algorithm in tandem with large datasets of variant annotations and gene functions for genetic variations with pharmacogenomics impact was developed. A comprehensive pathway map of drug transport, metabolism and action was used as a template to map non-synonymous variations with potential functional consequences.Principal Findings
Over 3 million known variations and 100,898 novel variations in the Malay genome were identified. Further in-depth pharmacogenetics analysis revealed a total of 607 unique variants in 563 proteins, with the eventual identification of 4 drug transport genes, 2 drug metabolizing enzyme genes and 33 target genes harboring deleterious SNVs involved in pharmacological pathways, which could have a potential role in clinical settings.Conclusions
The current study successfully unravels the potential of personal genome sequencing in understanding the functionally relevant variations with potential influence on drug transport, metabolism and differential therapeutic outcomes. These will be essential for realizing personalized medicine through the use of comprehensive computational pipeline for systematic data mining and analysis. 相似文献2.
Muhamad Aidilfitri Mohamad Roslan Nur Qaiyyum Ummi Aiman Jefri Nurhidayah Ramlee Nor Aini Abdul Rahman Nur Hazlin Hazrin Chong Hamidun Bunawan Izwan Bharudin Mohd. Hafiz Abd. Kadir Masita Mohammad Halim Razali 《Saudi Journal of Biological Sciences》2021,28(5):3001-3012
Food waste (FW) minimization at the source by using food waste biodigester (FWBs) has a vast potential to lower down the impact of increasing organic fraction in municipal solid waste generation. To this end, this research sought to check the performance of locally isolated hydrolase-producing bacteria (HPB) to improve food waste biodegradation rate. Two under-explored HPB identified as Bacillus paralicheniformis GRA2 and Bacillus velezensis TAP5 were able to produce maximum amylase, cellulase, protease and lipase activities, and demonstrated a significant hydrolase synergy in co-culture fermentation. In vitro biodegradation analysis of both autoclaved and non-autoclaved FW revealed that the HPB inoculation was effective to degrade total solids (>62%), protein (>19%), total fat (>51), total sugar (>86%), reducing sugar (>38%) and starch (>50%) after 8-day incubation. All co-culture treatments were recorded superior to the respective monocultures and the uninoculated control. The results of FW biodegradation using batch-biodigester trial indicated that the 1500 mL and 1000 mL inoculum size of HPB inoculant reached a plateau on the 4th day, with gross biodegradation percentage (GBP) of >85% as compared to control (66.4%). The 1000 mL inoculum was sufficient to achieve the maximum GBP (>90%) of FW after an 8-day biodigestion in a FWB. 相似文献
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Mohd Izwan Mohd Lazim Intan Safinar Ismail Khozirah Shaari Jalifah Abd. Latip Nabil Ali Al‐Mekhlafi Hiroshi Morita 《化学与生物多样性》2013,10(9):1589-1596
A chemical investigation of the alkaloidal fraction of Dysoxylum acutangulum leaves led to the isolation and characterization of two new chromone alkaloid analogs named chrotacumines E and F ( 1 and 2 , resp.). Structure elucidation of 1 and 2 was achieved by spectroscopic analyses, including 2D‐NMR. Both of these alkaloids exhibited modest activities as tyrosinase inhibitors with 29.2 and 25.8% inhibition at 100 μg/ml, respectively. 相似文献
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Noor Haza Fazlin Hashim Izwan Bharudin Douglas Law Sie Nguong Sakura Higa Farah Diba Abu Bakar Sheila Nathan Amir Rabu Hidehisa Kawahara Rosli Md. Illias Nazalan Najimudin Nor Muhammad Mahadi Abdul Munir Abdul Murad 《Extremophiles : life under extreme conditions》2013,17(1):63-73
The psychrophilic yeast Glaciozyma antarctica demonstrated high antifreeze activity in its culture filtrate. The culture filtrate exhibited both thermal hysteresis (TH) and ice recrystallization inhibition (RI) properties. The TH of 0.1 °C was comparable to that previously reported for bacteria and fungi. A genome sequence survey of the G. antarctica genome identified a novel antifreeze protein gene. The cDNA encoded a 177 amino acid protein with 30 % similarity to a fungal antifreeze protein from Typhula ishikariensis. The expression levels of AFP1 were quantified via real time-quantitative polymerase chain reaction (RT-qPCR), and the highest expression levels were detected within 6 h of growth at ?12 °C. The cDNA of the antifreeze protein was cloned into an Escherichia coli expression system. Expression of recombinant Afp1 in E. coli resulted in the formation of inclusion bodies that were subsequently denatured by treatment with urea and allowed to refold in vitro. Activity assays of the recombinant Afp1 confirmed the antifreeze protein properties with a high TH value of 0.08 °C. 相似文献
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Arentz G Chataway T Price TJ Izwan Z Hardi G Cummins AG Hardingham JE 《Clinical proteomics》2011,8(1):16
Introduction
Biomarkers that improve stratification of colorectal cancer patients for adjuvant therapy versus resection alone, or that are predictive of response to therapeutic agents, have the potential to greatly improve patient selection for such therapies. The aim was to determine proteins differentially expressed within the malignant epithelial glands and closely associated stromal elements compared to matched normal mucosa, and to characterise the over-expression of one such protein as a potential biomarker. 相似文献
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