Comparative research on erythrocyte anionic adenosine triphosphatase in vertebrates

Studies have been made on the level of activity of anion ATPase and its sensitivity to anions in erythrocytic membranes from fishes, amphibians, reptiles, birds and mammals. Significant variations in these properties of the ATPase were found among the species investigated. Protein composition of erythrocytic membranes was also investigated by means of disc-electrophoresis in polyacrylamide gel in men, rabbit, rat, mouse, hamster, tortoise, crow and starling.     

Effect of physico-chemical factors on anion-sensitive ATPase activity

Effects of temperature, pH and anions on the ATPase activity of submitochondrial particles of rat liver, rat heart, mouse liver, of red blood cell membranes, and of soluble enzyme of rat liver, mouse liver mitochondria were studied. The temperature relationships of membrane-bound and soluble ATPases have the breaks at 18-21 degrees C and 30-32 degrees C. These breaks were not shifted by sulfite, thiocyanate, methanol, glycerol and GTP. The pH changes from 6.0 to 8.5 produced no effect on the temperature relationships of ATPase activities but, strongly influenced the rate of ATPase reaction. The conformity between the obtained data and earlier proposed mechanism of anion control over anion-sensitive ATPase activity was discussed.     

Polymorphism of nucleotide sequences of human genomic DNA linked to a mucoviscidosis locus

Polymorphism in the restriction fragments length of human DNA sequences linked to mucoviscidosis locus was studied in the healthy control group and in the families affected by mucoviscidosis. The plasmid clones metH, pJ3.11,XV-2c and pKM.19 were used as hybridization probes. The allelic frequencies of the polymorphic loci were determined for total population and for affected families. The linkage disequilibrium between the disease locus and linked polymorphic loci detectable with XV-2c (TaqI endonuclease) and pKM.19 (PstI endonuclease) was demonstrated. The high informational value of DNA-diagnosis of mucoviscidosis in the family studies with the use of four DNA probes combination has been demonstrated.     

酪氨酸对大鼠子宫胞浆雌,孕激素受体的影响

采用放射受体分析法,测定了动情周期不同阶段及去卵巢大鼠子宫胞浆雌二醇和孕酮受体含量,并观察了子宫腔内注射酪、丝、苏三种氨基酸对子宫胞浆雌。醇、孕酮受体含量的影响。结果表明：（１）Ｌ－酪氨酸对动情前期、动情期、间情期大鼠子宫胞浆雌二醇和孕酮受体都具有明显的降低作用。（２）Ｌ－酪氨酸也降低去卵巢大鼠子宫胞浆雌二醇和孕酮含量,即这一作用不是通过影响卵巢激素分泌实现的。（３）Ｌ－苏氨酸仅可降低动情期和间情期大鼠子宫胞浆孕酮受体含量,而对相应周期雌二醇受体没有明显作用。（４）Ｌ－丝氨酸和Ｌ－苏氨酸对去卵巢大鼠子宫胞浆雌二醇和孕酮受体均无影响。     

人胚DA能神经元移植于帕金森氏病猴模型的TH免疫细胞化学的研究

为了对人胚黑质ＤＡ神经元移植治疗ＰＤ人的临床应用作出客观评估,将８－１２周人胚黑质细胞移植到用ＭＰＴＰ诱发的偏侧ＰＤ猴新纹状体内。实验动物分别存活２个月、５个月和１年后,用ＴＨ免疫细胞化学方法对被移植的人胚ＤＡ细胞的存活和与宿主间的突触联系进行检查。在光镜下可见被移植侧的新纹状体内有ＴＨ阳性细胞,它们成小群散在分布,每小群有３－１０个细胞。ＴＨ阳性细胞的轴突延伸到整个新纹状体,树突呈现出正常发育过     

Effect of verbal reinforcement on evoked cortical activity

Adult healthy subjects did not manifest any difference in latency and amplitude of the wave P300 elicited by a positive ("good") and negative ("error") reinforcing stimuli. After the negative reinforcement, the P300 wave amplitude decreases in response to the standard stimulus (light bars) and increases to a lesser degree in response to test stimuli (the same bars but presented with different pauses). In the processes of learning to assess time microintervals in comparison with the standard, the latency of wave P300 to the test stimuli shortens. It is suggested that formation and consolidation of feedback connection elaborated with the participation of a reinforcing verbal stimulus constitute the physiological basis for learning of comparative assessment of time microintervals.     

Changes in the mitochondrial ATPase activity of liver cells after X-ray irradiation

24 h following total-body X-irradiation of rats with a dose of 7 Gy the growth of the ATPase activity diminished, with the addition of bicarbonate, by 2 times in the preparations of mitochondria and submitochondrial particles, and by 1.5 times, in preparations of a soluble enzyme. No changes were noted in the electrophoretic motility of soluble ATPase.     

Changes in the membrane ATPase activity of erythrocytes and Candida guilliermondii under the action of roseofungin

Changes in the Mg-ATPase and Na, K-ATPase activity of the rat erythrocyte and Candida guilliermondii membranes under the effect of roseofungin were studied. The antibiotic was totally bound to the isolated plasmatic membranes of Candida guilliermondii, up to 3 micrograms of the antibiotic per 1 microgram of the yeast protein. The Mg-APTase activity of these membranes was slightly inhibited by the antibiotic. The activity of Na, K-ATPase was almost completely inhibited even at 0.04 mg of roseofungin per 1 mg of protein. Much higher concentrations of the antibiotic inhibited the Mg-ATPase and Na, K-ATPase activity of the erythrocyte membranes to a less extent.     

斑节对虾精子发生的超微结构

斑节对虾精子发生划分为精原细胞、初级精母细胞、次级精母细胞、精子细胞和精子五个阶段。精子发生中，从精原细胞到精子，染色质经历了从以异染色质为主变为高度凝聚态，再经解聚为弥散絮状的变化过程。同时，核从具有完整核膜变为核膜不完整。成熟的的精子含有核仁。顶体由高尔基囊泡逐渐演化而成，并向外伸长成为棘突。这是斑节对虾精子发生的主要特征。     

Proliferation of the intestinal epithelium and of the regenerating liver with a deficiency of the epidermal growth factor

To determine the role of the epidermal growth factor (EGF) in the regenerative stimulation of intestinal epithelium and hepatocyte proliferation after partial resection of these organs the labeling index of the intestine and liver in sialadenectomized rats was studied. EGF concentration in the saliva and serum was determined using radioimmunoassay. The decrease in EGF concentration after the removal of submandibular salivary glands was shown to slower hepatocytes entering the mitotic cycle and to inhibit the activity of enterocyte proliferation in the small intestine. The data show pronounced in vivo mitogenic effect of EGF on the liver and intestinal epithelium.