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排序方式: 共有196条查询结果,搜索用时 15 毫秒
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2.
Christian Alvarez Glaucia Gon?alves Barbosa Raquel de Vasconcellos Carvalhaes de Oliveira Bernardina Penarrieta Morales Bodo Wanke Márcia dos Santos Lazéra 《Memórias do Instituto Oswaldo Cruz》2013,108(1):126-129
In this study, we evaluated several techniques for the detection of the yeast form of Cryptococcus in decaying wood and measured the viability of these fungi in environmental samples stored in the laboratory. Samples were collected from a tree known to be positive for Cryptococcus and were each inoculated on 10 Niger seed agar (NSA) plates. The conventional technique (CT) yielded a greater number of positive samples and indicated a higher fungal density [in colony forming units per gram of wood (CFU.g-1) ] compared to the humid swab technique (ST). However, the difference in positive and false negative results between the CT-ST was not significant. The threshold of detection for the CT was 0.05.103 CFU.g-1, while the threshold for the ST was greater than 0.1.103 CFU-1. No colonies were recovered using the dry swab technique. We also determined the viability of Cryptococcus in wood samples stored for 45 days at 25ºC using the CT and ST and found that samples not only continued to yield a positive response, but also exhibited an increase in CFU.g-1, suggesting that Cryptococcus is able to grow in stored environmental samples. The ST.1, in which samples collected with swabs were immediately plated on NSA medium, was more efficient and less laborious than either the CT or ST and required approximately 10 min to perform; however, additional studies are needed to validate this technique. 相似文献
3.
The immediate-early growth response in regenerating liver and insulin-stimulated H-35 cells: comparison with serum-stimulated 3T3 cells and identification of 41 novel immediate-early genes. 总被引:30,自引:8,他引:22
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K L Mohn T M Laz J C Hsu A E Melby R Bravo R Taub 《Molecular and cellular biology》1991,11(1):381-390
Liver regeneration provides a unique system for analysis of mitogenesis in intact, fully developed animals. Cellular immediate-early genes likely play an important role in cell cycle regulation and have been extensively studied in mitogen-stimulated fibroblasts lymphocytes but not in liver. We have begun to characterize the immediate-early growth response genes of mitogen-stimulated liver cells, specifically, regenerating liver and insulin-stimulated Reuber H-35 hepatoma cells, and to address differences in growth response between different cell types. Through subtraction and differential screening of cDNA libraries from regenerating liver and insulin-treated H-35 cells, we have extensively characterized 341 differentially expressed clones and identified 52 immediate-early genes. These genes have been partially sequenced and subjected to Northern (RNA) blot analysis, and 41 appear to be novel. Surprisingly, two-thirds of these genes are also expressed in BALB/c 3T3 cells, but only 10 were identified in previous studies of 3T3 cells, and of these, 6 include well-known genes like jun and fos, and only 4 are novel. Approximately one-third of the immediate-early genes identified in mitogen-stimulated liver cells or serum-stimulated NIH 3T3 cells are expressed in a tissue-specific fashion, indicating that cell type-specific regulation of the proliferative response occurs during the immediate-early period. Our findings indicate that the immediate-early response is unusually complex for the first step in a regulatory cascade, suggesting that multiple pathways must be activated. The abundance of immediate-early genes and the highly varied pattern of their expression in different cell types suggest that the tissue specificity of the proliferative response arises from the particular set of these genes expressed in a given tissue. 相似文献
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Anthony J. Petrella Jeffrey R. Armstrong Peter J. Laz Paul J. Rullkoetter 《Computer methods in biomechanics and biomedical engineering》2013,16(11):1223-1232
Wear testing of polyethylene in total joint replacements is common and required for any new device. Computational wear modelling has obvious utility in this context as it can be conducted with much greater economy than physical testing. Archard's law has become the accepted standard for wear simulation in total joints but it does not account for cross-shear, which is known to increase wear significantly relative to unidirectional sliding. The purpose of this study was to develop a robust cross-shear model applicable to any interface geometry under any kinematic conditions. The proposed metric, x *, is distinguished from existing cross-shear models by the fact that it measures cross-path motion incrementally throughout a motion cycle and quantifies cross-shear based on incremental changes in sliding direction. Validation showed strong support for the predictive capability of x * when applied to pin-on-disc test data. 相似文献
7.
Hyojung Cha George Fish Joel Luke Ahmad Alraddadi Hyun Hwi Lee Weimin Zhang Yifan Dong Saurav Limbu Andrew Wadsworth Iuliana P. Maria Laia Francs Hou Lon Sou Tian Du Ji‐Seon Kim Martyn A. McLachlan Iain McCulloch James R. Durrant 《Liver Transplantation》2019,9(27)
Here, it is investigated whether an energetic cascade between mixed and pure regions assists in suppressing recombination losses in non‐fullerene acceptor (NFA)‐based organic solar cells. The impact of polymer‐NFA blend composition upon morphology, energetics, charge carrier recombination kinetics, and photocurrent properties are studied. By changing film composition, morphological structures are varied from consisting of highly intermixed polymer‐NFA phases to consisting of both intermixed and pure phase. Cyclic voltammetry is employed to investigate the impact of blend morphology upon NFA lowest unoccupied molecular orbital (LUMO) level energetics. Transient absorption spectroscopy reveals the importance of an energetic cascade between mixed and pure phases in the electron–hole dynamics in order to well separate spatially localized electron–hole pairs. Raman spectroscopy is used to investigate the origin of energetic shift of NFA LUMO levels. It appears that the increase in NFA electron affinity in pure phases relative to mixed phases is correlated with a transition from a relatively planar backbone structure of NFA in pure, aggregated phases, to a more twisted structure in molecularly mixed phases. The studies focus on addressing whether aggregation‐dependent acceptor LUMO level energetics are a general design requirement for both fullerene and NFAs, and quantifying the magnitude, origin, and impact of such energetic shifts upon device performance. 相似文献
8.
Sicora Cosmin Ionel Chiș Iuliana Chiș Ciprian Sicora Oana 《Photosynthesis research》2019,139(1-3):461-473
Photosynthesis Research - Cyanobacteria, as well as green algae and higher plants, have highly conserved photosynthetic machinery. Cyanothece sp. ATCC 51142 is a unicellular, aerobic, diazotrophic... 相似文献
9.
Chlorophyll a fluorescence induction measured by a fluorometer with a high temperature stressed plant material shows a new K step which
is a clear peak due to fast fluorescence rise and subsequent decrease of fluorescence intensity. We focused on an explanation
of the decrease of fluorescence after the K step using artificial electron acceptors and donors to photosystem 2 (PS2). Addition
of the artificial electron acceptors or donors suppressed the decrease of fluorescence after the K step. We suggest that the
decrease mainly reflects (by more than 81 %) an energy loss process in the reaction centre of PS2 which is most probably a
nonradiative charge recombination between P680+ (oxidised primary electron donor in PS2) and a negative charge stored on either Pheo− or QA
− (reduced primary electron acceptor of PS2 and reduced primary quinone electron acceptor of PS2, respectively). We suggest
that the energy loss process is only possible when the inhibition of both the donor and the acceptor sides of PS2 occurs.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
10.
Lazárová M Stejskal D Lacnák B Václavík J Adamovská S Ochmanová R Hanák V Skácelová M 《Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia》2004,148(2):131-133
Oxidative stress and especially its connection with many diseases has been discussed much recently. Among markers of oxidative stress there appear new and quite specific ones called advanced oxidation protein products (AOPPs). We tried to influence the level of AOPPs by an antioxidant therapy with N-acetylcysteine. Fourteen individuals with many cardiovascular risk factors were examined. All these patients were administered acetylcysteine (NAC) 600 mg/day orally during 20 days. Before starting the therapy we determined AOPP, albumin cobalt binding (ACB), glucose, creatinine, urea, ALT, AST, cholesterol, LDL, HDL and triglycerides values in peripheral venous blood in all individuals. After finishing our intervention we determined AOPP, ACB and glucose level again. Our results show a statistically significant decrease in AOPP levels after 20-day N-acetylcysteine therapy (medians, initially 82.2, at study end 74.3 umol/l, p = 0.039). We demonstrate a significant decrease in AOPP levels after 20-day N-acetylcysteine therapy in dose 600 mg/day. 相似文献