全文获取类型
收费全文 | 714篇 |
免费 | 63篇 |
专业分类
777篇 |
出版年
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 8篇 |
2020年 | 11篇 |
2019年 | 6篇 |
2018年 | 11篇 |
2017年 | 7篇 |
2016年 | 15篇 |
2015年 | 13篇 |
2014年 | 24篇 |
2013年 | 31篇 |
2012年 | 47篇 |
2011年 | 29篇 |
2010年 | 28篇 |
2009年 | 24篇 |
2008年 | 34篇 |
2007年 | 32篇 |
2006年 | 36篇 |
2005年 | 23篇 |
2004年 | 26篇 |
2003年 | 32篇 |
2002年 | 22篇 |
2001年 | 12篇 |
2000年 | 15篇 |
1999年 | 13篇 |
1998年 | 4篇 |
1997年 | 11篇 |
1996年 | 10篇 |
1995年 | 7篇 |
1994年 | 10篇 |
1993年 | 8篇 |
1992年 | 18篇 |
1991年 | 16篇 |
1990年 | 17篇 |
1989年 | 16篇 |
1988年 | 20篇 |
1987年 | 5篇 |
1986年 | 16篇 |
1985年 | 10篇 |
1984年 | 10篇 |
1983年 | 11篇 |
1982年 | 5篇 |
1980年 | 5篇 |
1977年 | 9篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1974年 | 3篇 |
1969年 | 5篇 |
1968年 | 5篇 |
1902年 | 2篇 |
排序方式: 共有777条查询结果,搜索用时 0 毫秒
1.
2.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
- Download : Download high-res image (147KB)
- Download : Download full-size image
- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
3.
4.
5.
6.
Muscarinic Agonists Evoke Neurotransmitter Release: Possible Roles for Phosphatidyl Inositol Bisphosphate Breakdown Products in Neuromodulation 总被引:1,自引:0,他引:1
Sophia Diamant Itzhak Lev-Ari Illana Uzielli Daphne Atlass 《Journal of neurochemistry》1988,51(3):795-802
Carbachol (CCh), a muscarinic agonist that elicits the formation of inositol trisphosphate (IP3) and diacylglycerol (DG), induces a calcium-dependent [3H]norepinephrine ([3H]NE) release [IC50 = (2.7 +/- 0.5) X 10(-4) M] in rat brain slices. Similarly, other muscarinic agonists evoke [3H]NE release which is specifically inhibited by muscarinic antagonists such as 3-quinuclidinyl benzilate, atropine, and N-methyl-4-piperidyl benzilate. The atropine-sensitive evoked release is effectively inhibited by neomycin (IC50 = 50 microM), a phospholipase C inhibitor that interferes with IP3-dependent cellular processes. In addition, polymyxin B, a rather selective inhibitor of protein kinase C (PK-C), abolishes the agonist-mediated release with a half-maximal effective concentration of 0.53 microM (750 ng/ml). These results have a significant implication for the mechanism by which agonists generating IP3 and DG act as inducers of neurotransmitter release in the CNS. However, since both neomycin and polymyxin B act also as N-calcium-channel blockers, other possible mechanisms are discussed. The CCh-induced release suggests that in the CNS an agonist-receptor interaction leads to a calcium-dependent neurotransmitter release, most likely via promoting the IP3/DG as second messengers followed by activation of PK-C. 相似文献
7.
Victor S. Sapirstein Charles E. Nolan Itzhak Fischer Elizabeth Cochary Susana Blau Cheryl J. Flynn 《Neurochemical research》1991,16(2):123-128
Plasmolipin is a plasma membrane proteolipid is a major myelin membrane component (Cochary et al., 1990). In this study we report the phylogenic expression of plasmolipin in the vertebrate nervous system. Using Western blot analysis with polyclonal antibodies, we have analyzed membrane fractions, including myelin, from elasmobranchs, teleosts, amphibians, reptiles, birds and mammals. On the basis of immune detection, plasmolipin appears to be restricted to the mammalian nervous system. Comparison of the central and peripheral nervous systems of mammals showed only minor differences in the level of plasmolipin in these two regions. Within mammals, little quantitative differences were observed when rat, human and bovine membrane fractions were compared. The late evolutionary expression of plasmolipin which results in its restriction to mammals makes it unique among the (major) myelin proteins. The potential physiologic significance of these data are discussed.Abbreviations EDTA
Ethylene diamine N.,NN tetracetic acid
- EGTA
Ethylene glycol bis-(B-Aminoethyl Ether) N,,NN tetracetic acid
- MES
([N-Morpholino] ethane sulfonic acid) DCCD, N, Dicyclohexyl carbodiimide 相似文献
8.
Morphological changes in clostridial isolates after animal passage with other flora in mixed infections were studied by utilizing a subcutaneous abscess model in mice. We used 26 isolates of 7 clostridial species, and one isolate each of Bacteroides fragilis and Klebsiella pneumoniae. Abscesses were induced by all 7 Clostridium perfringens and 3 Clostridium butyricum isolates and by some of the other isolates. A thick granular wall prior to animal inoculation was shown only in C. perfringens, C. butyricum, and C. difficile. This structure was observed in other clostridia only following their animal passage alone or when co-inoculated with K. pneumoniae or B. fragilis. 相似文献
9.
The pathogenicity of 22 anaerobic and facultative Gram-positive cocci (AFGPC) was investigated by inoculating them into mice and determining their ability to cause subcutaneous abscesses. Only 11 heavily encapsulated isolates (greater than 50% of the cells were encapsulated) induced abscesses. However, when the other 11 isolates were injected with Bacteroides sp. or facultative and aerobic bacteria, abscesses were formed in 8 of the 11 combinations. The AFGPC recovered from the mixed infections contained many encapsulated cells. Encapsulation also occurred in cocci injected with capsular material or with Formalin-killed cells of Klebsiella pneumoniae or capsule-positive Bacteroides sp. After acquisition of capsules, these strains could induce abscesses on reinoculation in mice. 相似文献
10.
Regional localisations and linkage relationships of seven RFLPs and myotonic dystrophy on chromosome 19 总被引:4,自引:2,他引:2
D. J. Shaw A. L. Meredith M. Sarfarazi H. G. Harley S. M. Huson J. D. Brook L. Bufton M. Litt T. Mohandas P. S. Harper 《Human genetics》1986,74(3):262-266
Summary We have studied the genetic linkage relationships of seven DNA polymorphisms on chromosome 19, with each other and with the myotonic dystrophy locus. The DNA sequences were localised to various regions of the chromosome using translocations in somatic cell hybrids. These results provide the basis for a linkage map of most of chromosome 19, and suggest that the myotonic dystrophy locus is close to the centromere. 相似文献