全文获取类型
收费全文 | 3522篇 |
免费 | 237篇 |
国内免费 | 1篇 |
专业分类
3760篇 |
出版年
2023年 | 13篇 |
2022年 | 34篇 |
2021年 | 50篇 |
2020年 | 46篇 |
2019年 | 40篇 |
2018年 | 45篇 |
2017年 | 41篇 |
2016年 | 93篇 |
2015年 | 144篇 |
2014年 | 162篇 |
2013年 | 230篇 |
2012年 | 283篇 |
2011年 | 261篇 |
2010年 | 143篇 |
2009年 | 142篇 |
2008年 | 212篇 |
2007年 | 208篇 |
2006年 | 195篇 |
2005年 | 186篇 |
2004年 | 175篇 |
2003年 | 158篇 |
2002年 | 165篇 |
2001年 | 38篇 |
2000年 | 27篇 |
1999年 | 32篇 |
1998年 | 36篇 |
1997年 | 41篇 |
1996年 | 19篇 |
1995年 | 22篇 |
1994年 | 30篇 |
1993年 | 31篇 |
1992年 | 29篇 |
1991年 | 22篇 |
1990年 | 19篇 |
1989年 | 23篇 |
1988年 | 23篇 |
1987年 | 17篇 |
1986年 | 20篇 |
1985年 | 18篇 |
1984年 | 22篇 |
1983年 | 20篇 |
1982年 | 23篇 |
1981年 | 19篇 |
1980年 | 22篇 |
1979年 | 15篇 |
1978年 | 15篇 |
1977年 | 15篇 |
1976年 | 20篇 |
1975年 | 15篇 |
1974年 | 21篇 |
排序方式: 共有3760条查询结果,搜索用时 15 毫秒
1.
2.
Abstract. Soil resource availability may affect plant regeneration by resprouting in disturbed environments directly, by affecting plant growth rates, or indirectly by determining allocation to storage in the resprouting organs. Allocation to storage may be higher in stressful, low resource‐supply soils, but under such conditions plant growth rates may be lower. These factors could act in opposite directions leading to poorly known effects on resprouting. This paper analyses the role played by soil resources in the production and growth of resprouts after removal of above‐ground plant tissues in the Mediterranean shrub Erica australis. At 13 sites, differing in substrate, we cut the base of the stems of six plants of E. australis and allowed them to resprout and grow for two years. Soils were chemically analysed and plant water potential measured during the summer at all sites to characterize soil resource availability. We used stepwise regression analysis to determine the relationships between the resprouting response [mean site values of the number of resprouts (RN), maximum length (RML) and biomass (RB)] and soil nutrient content and plant water potential at each site. During the first two years of resprouting there were statistically significant differences among sites in the variables characterizing the resprouting response. RML was always different among sites and had little relationship with lignotuber area. RN was less different among sites and was always positively correlated with lignotuber area. RB was different among sites after the two years of growth. During the first months of resprouting, RN and RML were highly and positively related to the water status of the plant during summer. At later dates soil fertility variables came into play, explaining significant amounts of variance of the resprouting variables. Soil extractable cations content was the main variable accounting for RML and RB. Our results indicate that resprout growth of E. australis is positively affected by high water availability at the beginning of the resprouting response and negatively so by high soil extractable cation content at later periods. Some of these factors had previously shown to be related, with an opposite sign, to the development of a relatively larger lignotuber. Indeed, RML and RB measured in the second year of resprouting were significantly and negatively correlated with some indices of biomass allocation to the lignotuber at each site. This indicates that sites favouring allocation to the resprouting organ may not favour resprout growth. 相似文献
3.
Anna M. Kauppi Alicia Edin Ingrid Ziegler Paula M?lling Anders Sj?stedt ?sa Gylfe Kristoffer Str?lin Anders Johansson 《PloS one》2016,11(1)
A metabolomics approach for prediction of bacteremic sepsis in patients in the emergency room (ER) was investigated. In a prospective study, whole blood samples from 65 patients with bacteremic sepsis and 49 ER controls were compared. The blood samples were analyzed using gas chromatography coupled to time-of-flight mass spectrometry. Multivariate and logistic regression modeling using metabolites identified by chromatography or using conventional laboratory parameters and clinical scores of infection were employed. A predictive model of bacteremic sepsis with 107 metabolites was developed and validated. The number of metabolites was reduced stepwise until identifying a set of 6 predictive metabolites. A 6-metabolite predictive logistic regression model showed a sensitivity of 0.91(95% CI 0.69–0.99) and a specificity 0.84 (95% CI 0.58–0.94) with an AUC of 0.93 (95% CI 0.89–1.01). Myristic acid was the single most predictive metabolite, with a sensitivity of 1.00 (95% CI 0.85–1.00) and specificity of 0.95 (95% CI 0.74–0.99), and performed better than various combinations of conventional laboratory and clinical parameters. We found that a metabolomics approach for analysis of acute blood samples was useful for identification of patients with bacteremic sepsis. Metabolomics should be further evaluated as a new tool for infection diagnostics. 相似文献
4.
5.
Yanyan Han Elfriede Eppinger Ingrid G. Schuster Luise U. Weigand Xiaoling Liang Elisabeth Kremmer Christian Peschel Angela M. Krackhardt 《The Journal of biological chemistry》2009,284(48):33409-33417
The formin protein formin-like 1 (FMNL1) is highly restrictedly expressed in hematopoietic lineage-derived cells and has been previously identified as a tumor-associated antigen. However, function and regulation of FMNL1 are not well defined. We have identified a novel splice variant (FMNL1γ) containing an intron retention at the C terminus affecting the diaphanous autoinhibitory domain (DAD). FMNL1γ is specifically located at the cell membrane and cortex in diverse cell lines. Similar localization of FMNL1 was observed for a mutant lacking the DAD domain (FMNL1ΔDAD), indicating that deregulation of autoinhibition is effective in FMNL1γ. Expression of both FMNL1γ and FMNL1ΔDAD induces polarized nonapoptotic blebbing that is dependent on N-terminal myristoylation of FMNL1 but independent of Src and ROCK activity. Thus, our results describe N-myristoylation as a regulative mechanism of FMNL1 responsible for membrane trafficking potentially involved in a diversity of polarized processes of hematopoietic lineage-derived cells. 相似文献
6.
Franz Peter Fischer Christina Miltz Ingrid Singer Geoffrey A. Manley 《Journal of morphology》1992,213(2):225-240
The starling cochlea was studied with TEM at four locations along the basilar papilla to investigate gradients in morphological features over the papilla's length and width. Hair cell shape changes continuously from neural to abneural and from basal to apical. Unlike the situation in mammals, there are no distinct populations of hair cells; the previously described types (tall hair cells and short hair cells) are merely extremes in a continuum. Contacts between THC are a normal feature. Except at the base of the papilla, SHC have very large cuticular plates, suggesting a micromechanical function for these cells. In contrast to the THC, the SHC normally completely lack afferent innervation; this indicates that their function is restricted to within the basilar papilla itself. © 1992 Wiley-Liss, Inc. 相似文献
7.
Quintana Xavier D.; Comin Francisco A.; Moreno-Amich Ramon 《Journal of plankton research》2002,24(11):1149-1161
The distribution of biomass among phytoplankton and free-livinginvertebrates was analysed in a shallow Mediterranean salt marshsubmitted to fluctuating water level. Among phytoplankton, biomassaccumulated in sizes dominated by mixotrophic species, indicatinga competitive advantage for these species, which also prey onother smaller primary producers. Among invertebrates, biomassaccumulated in the larger sizes, corresponding to species whichpartially exploit other nearby systems, such as the aerial environment(insects), or to those able to exploit particulated organicmatter in marsh-bed sediment (amphipods). Biomass distributionmodels developed for pelagic systems are discussed in relationto fluctuating temporary waters. The integrated spectrum approximated(r2 = 0.96) a Pareto distribution with a slope of c = 1.38.Intense disturbances caused a decrease in r2 and an increasein c. Under stable conditions, two different tendencies wereobserved, depending on the degree of eutrophy of the basins:higher values of c were measured in the more eutrophic basins,and lower values in the less eutrophic ones. We hypothesizethat highly irregular nutrient input could explain these differences. 相似文献
8.
Abstract. 1. Pollen is considered to be an important dietary component for many species of flower-feeding herbivores. Its influence on oviposition site selection by the pollen beetle Meligethes aeneus , and on the development of its larvae was investigated.
2. The effects of pollen presence and absence on adult, egg, and larval incidence in the field, and on larval development in the laboratory were compared through the use of Synergy, a composite hybrid oilseed rape Brassica napus variety comprising male-fertile (with pollen) and male-sterile (without pollen) plants.
3. In the field, adult females were more abundant on male-fertile plants during flowering, and a greater proportion of male-fertile than male-sterile buds were accepted for oviposition. These data indicate a possible role of pollen in oviposition site selection by female pollen beetles.
4. The numbers of first instar larvae on the two plant lines did not differ; however, more second instars were found on male-fertile than on male-sterile flowers. This suggests a greater larval survival on male-fertile plants, possibly due to the more readily available food resources and better nutrition afforded by the presence of pollen.
5. Laboratory experiments confirmed that a diet which included pollen improved survival to adulthood and resulted in heavier pupae and adults; however, pollen was not obligatory for larval survival and development.
6. The pollen beetle, previously thought to be an obligate pollen feeder, is therefore more generalist in its requirements for development. These findings may relate to the nutritional and behavioural ecology of other flower-feeding herbivores. 相似文献
2. The effects of pollen presence and absence on adult, egg, and larval incidence in the field, and on larval development in the laboratory were compared through the use of Synergy, a composite hybrid oilseed rape Brassica napus variety comprising male-fertile (with pollen) and male-sterile (without pollen) plants.
3. In the field, adult females were more abundant on male-fertile plants during flowering, and a greater proportion of male-fertile than male-sterile buds were accepted for oviposition. These data indicate a possible role of pollen in oviposition site selection by female pollen beetles.
4. The numbers of first instar larvae on the two plant lines did not differ; however, more second instars were found on male-fertile than on male-sterile flowers. This suggests a greater larval survival on male-fertile plants, possibly due to the more readily available food resources and better nutrition afforded by the presence of pollen.
5. Laboratory experiments confirmed that a diet which included pollen improved survival to adulthood and resulted in heavier pupae and adults; however, pollen was not obligatory for larval survival and development.
6. The pollen beetle, previously thought to be an obligate pollen feeder, is therefore more generalist in its requirements for development. These findings may relate to the nutritional and behavioural ecology of other flower-feeding herbivores. 相似文献
9.
Frank M. Maas Luit J. de Kok Ingrid Hoffmann Pieter J. C. Kuiper 《Physiologia plantarum》1987,70(4):713-721
Exposure of spinach (Spinacia oleracea L. cv. Monosa) to 0.25 μl l?1 H2S reduced the relative growth rate by 26, 47 and 60% at 15, 18 and 25°C, respectively. Shoot to root ratio decreased in plants fumigated at 18 and 25°C. Growth of spinach was not affected by a 2-week exposure to 0.10 or 0.25 μl l?1 SO2. Both H2S and SO2 fumigation increased the content of sulfhydryl compounds and sulfate. A 2-week exposure to 0.25 μl l?1 H2S resulted in an increase in sulfhydryl and sulfate content of 250 to 450% and 63 to 248% in the shoots, respectively, depending on growth temperature. Exposure to 0.15 and 0.30 μl l?1 H2S at 20°C for 2 weeks resulted in a 46% increase in sulfate content of the shoots at 0.30 μl l?1 and no detectable increase at 0.15 μl l?1 H2S; the sulfate content of the roots increased by 195 and 145% at 0.15 and 0.30 μl l?1 H2S, respectively. Fumigation with 0.25 μl l?1 SO2 at 20°C for 2 weeks resulted in an increase in sulfhydryl content and sulfate content in the shoots of 285% and 300 to 1100%. H2S fumigation during the 12 h light period or only during the dark period resulted in identical growth reduction and accumulation of sulfhydryl compounds; they were about 50 and 67% of those observed in continuously exposed plants. H2S- and SO2-exposed plants showed an increased transpiration rate, which was mainly caused by an increased dark-period transpiration. No effect of H2S and SO2 on the water uptake of the plants and the osmotic potential of the leaves was detected. Plants fumigated with 0.25 μl l?1 H2S for 2 weeks were smaller and differed morphologically from the control plants by slightly more abaxially curved leaf margins. Cross sections of the leaves showed smaller cells at the margins and smaller and fewer air spaces. The increased transpiration in the H2S-exposed plants is discussed in relation to the observed morphological changes. 相似文献
10.
J. Čatský D. K. Velichkov Jana Pospísilová Jarmila Solárová Ingrid Tichá 《Biologia Plantarum》1987,29(5):355-364
The carbon balances of whole, 21-d old French bean plants (Phaseolus vulgaris L.) grown in standard nutrient solution (1K) and its modifications without (OK) or surplus (2K) potassium were calculated
from the daily photosynthetic carbon inputs of individual leaves, and the daily respiratory carbon losses by individual leaves,
stalks and petioles, and roots. Under the three K concentrations, maximum net photosynthetic rates (Pn) were found in the 2nd or in the 3rd trifoliate leaves, maximum respiratory rates (Rd) in the youngest, 4th trifoliate leaves; the Pn/Rd ratio decreased with leaf age. In all leaves of 2K plants, leaf dry masses and thicknesses, Pn, Pn/Pd ratios, and stomatal and intracellular conductances were lower than in OK and IK plants. Daily whole-plant net carbon gain
was highest in IK plants, whereas in OK and 2K plants it was 98.0 and 81.3 % of IK, respectively. Similar values were found
in the parameters of growth analysis, namely in net assimilation rates and relative growth rates.
No differences were found in water potential (Ψ
w
) or water saturation deficit (Wsat) in the OK, 1K and 2K plants sufficiently supplied with water or during wilting and resaturation. The decrease in Ψw to −0.97 MPa was associated with a 19.9 %, 31.4 % and 23.4 % decrease in Pn of OK, 1K and 2K plants, respectively, but no effect on Rd was found. In the three variants, the short-time effect of mild water stress was fully reversible. 相似文献