全文获取类型
收费全文 | 886篇 |
免费 | 42篇 |
国内免费 | 2篇 |
出版年
2023年 | 3篇 |
2022年 | 6篇 |
2021年 | 16篇 |
2020年 | 12篇 |
2019年 | 12篇 |
2018年 | 28篇 |
2017年 | 13篇 |
2016年 | 38篇 |
2015年 | 29篇 |
2014年 | 43篇 |
2013年 | 53篇 |
2012年 | 62篇 |
2011年 | 92篇 |
2010年 | 34篇 |
2009年 | 38篇 |
2008年 | 60篇 |
2007年 | 54篇 |
2006年 | 52篇 |
2005年 | 33篇 |
2004年 | 28篇 |
2003年 | 37篇 |
2002年 | 22篇 |
2001年 | 23篇 |
2000年 | 7篇 |
1999年 | 13篇 |
1998年 | 4篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 11篇 |
1990年 | 3篇 |
1989年 | 7篇 |
1988年 | 8篇 |
1987年 | 8篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 6篇 |
1982年 | 4篇 |
1979年 | 7篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 1篇 |
1970年 | 2篇 |
1969年 | 3篇 |
1968年 | 1篇 |
排序方式: 共有930条查询结果,搜索用时 406 毫秒
1.
2.
Study of the oviduct of the pied myna (Sturnus contra contra) throughout the year reveals that oviductal weight, length, surface epithelial height and glycogen content are low during August to January (nonbreeding phase), partially increase during February to April (pre-breeding phase), maximally increase in May (breeding phase) and decrease in June and July (post-breeding phase). In the nesting cycle, there is greatest growth in all the regions of the oviduct from early nest-building to the egg-laying period and this is followed by rapid involution during incubation and nestling periods. Some notable features in the oviduct of the pied myna are described: 1) All five regions of the oviduct (infundibulum, magnum, isthmus, uterus, and vagina) are clearly distinguishable when studied from serial sections of the oviduct even during the nonbreeding phase of the annual ovarian cycle. 2) There is a strong correlation between initiation of tubular gland formation and the onset of nestbuilding activity. 3) The distal part of the magnum is differentiated into a 'mucous region' having well developed basal nonciliated cells. 4) A sixth zone can be identified between the magnum and isthmus. Sperm hostlike glands exist at the cranial end of the zone. 5) Several circular epithelial invaginations are evident in the intermucosal folds and their size decreases in centripetal order in the vagina. 6) The pattern and degree of regression are different in various regions of the oviduct. A close synchrony between ovarian and oviducal cycles is indicated in the pied myna (Sturnus contra contra). 相似文献
3.
4.
Purification and immunological characterization of catfish (Heteropneustes fossilis) metallothionein 总被引:1,自引:0,他引:1
Catfish hepatic metallothionein was purified to homogeneity by Sephadex G-75 gel filtration, DEAE-Sephadex A-25 column chromatography and preparative polyacrylamide gel electrophoresis. Induction by cadmium and zinc, characteristic UV spectrum, cadmium binding property and its low MW established that it was a metallothionein. Antibody was raised in rabbit against catfish metallothionein. Catfish antimetallothionein cross-reacted with other fish metallothioneins but not with chicken or rodent metallothionein. Catfish metallothionein is more electronegative as compared to mouse, rat, chicken or hamster metallothionein. Catfish MT appeared to aggregate readily on storage and to be less electronegative. 相似文献
5.
Indu S. Ambudkar Timothy Lockwich Yukiharu Hiramatsu Bruce J. Baum 《Molecular and cellular biochemistry》1992,114(1-2):73-77
Conclusions While it is generally accepted that Ca2+ plays an important regulatory role in the physiology of a number of non-excitable cells, the mechanisms which regulate intracellular [Ca2+ are far from well established. Ca2+ transporting mechanisms which distribute Ca2+ intracellularly as well as those which allow influx of extracellular Ca2+ are involved in mediating intracellular Ca2+ homestasis. In this paper we have described recent studies on the regulation of the Ca2+ influx system in the data, it appears that the process of Ca2+ entry is extremely complex and may involve several levels of regulation. Understanding the molecular basis of these regulatory mechanisms presents a challeging problem for future studies. 相似文献
6.
Valerie J. Horn Paul A. Sheehy Miriam B. Goodman Indu S. Ambudkar 《Molecular and cellular biochemistry》1991,101(1):43-49
Intracellular Ca2+ mobilization events were assessed in mouse L cells, which contain native prostaglandin E1 receptors and transfected human 2 adrenergic receptors. Both Fura2 (single cell measurements) and Quin 2, (cuvette assays) were used to determine [Ca2+]i levels. Our results demonstrate that in the transfected cells there is a dose-dependent increase in [Ca2+]i in response to isoproterenol (0.1 nM–100 nM), which is inhibited by the -adrenergic antagonist, propranolol, and is a result of intracellular Ca2+ release. [Ca2+]1 in these cells was also increased by prostaglandin E1, 8 bromo cyclic AMP, and aluminum fluoride. Both 8 bromo cAMP and isoproterenol induced a rapid increase in the levels of IP1, IP2, and IP3. The data presented demonstrate that the elevation of intracellular cyclic AMP induces an increase in IP3 production which leads to an elevation in [Ca2+];. We propose that this cyclic AMP dependent activation of the IP3 generating system occurs at a post-receptor site.Abbreviations cAMP
Adenosine Cyclic 3-5-Monophosphate
- [Ca2+]i
intracellular [Ca2+]i
- 8 Br cAMP
8 Bromo Adenosine Cyclic 3-5-Monophosphate
- DAG
Diacylglycerol
- EGTA]
[Ethylene Bis (oxyethylenenitrilo)] Tetracetic acid
- BSA
Bovine Serum Albumin
- HBSS-H
Hanks' Balanced Salt Solution buffered with HEPES to pH 7.4
- HEPES
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
- PIP2
Phosphatidylinositol 4,5-bisphosphate
- IP2
Inositol 4 Phosphate
- IP2
Inositol 4,5 Bisphosphate
- IP3
Inositol Trisphosphate
- PGE1
Prostaglandin E1
- PBS
Phosphate Buffered Saline Solution 相似文献
7.
8.
R Diller M Iannone B R Cowen S Maiti R A Bogomolni R M Hochstrasser 《Biochemistry》1992,31(24):5567-5572
The photoinduced reaction cycle of bacteriorhodopsin (BR) has been studied by means of a recently developed picosecond infrared spectroscopic method at ambient temperature. BR - K difference spectra between 1560 and 1700 cm-1 have been recorded at delay times from 100 ps to 14 ns. The spectrum remains unchanged during this period. The negative difference OD band at 1660 cm-1 indicates the peptide backbone responds within 50 ps. A survey in the region of carboxylic side chain absorption around 1740 cm-1 reveals that perturbations of those groups, present in low-temperature FTIR spectra, are not observable within 10 ns, suggesting a slow conformational change. 相似文献
9.
Production of some extracellular enzymes by a lignin peroxidase-producing brown rot fungus, Polyporus ostreiformis, and its comparative abilities for lignin degradation and dye decolorization. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Polyporus ostreiformis produced Mn peroxidase, acid protease, alpha-amylase, and lignin peroxidase, with maximum activities of 40, 8,300, and 4,200 U liter-1 and 50 nkat liter-1, respectively, in nitrogen-limited liquid media. The fungus removed only 18.6% lignin from rice straw in 3 weeks but effected 99% decolorization of Congo red dye in 9 days. 相似文献
10.
Yukiharu Hiramatsu Indu S. Ambudkar Bruce J. Baum 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1991,1092(3):391-396
β-Adrenergic receptor stimulation of adenylyl cyclase involves the activation of a GTP-binding regulatory protein (G-protein, termed here Gs). Inactivation of this G-protein is associated with the hydrolysis of bound GTP by an intrinsic high affinity GTPase activity. In the present study, we have characterized the GTPase activity in a Gs-enriched rat parotid gland membrane fraction. Two GTPase activities were resolved; a high affinity GTPase activity displaying Michaelis-Menten kinetics with increasing concentrations of GTP, and a low affinity GTPase activity which increased linearly with GTP concentrations up to 10 mM. The β-adrenergic agonist isoproterenol (10 μM) increased the Vmax of the high affinity GTPase component approx. 50% from 90 to 140 pmol/mg protein per min, but did not change its Km value (≈ 450 nM). Isoproterenol also stimulated adenylyl cyclase activity in parotid membranes both in the absence or presence of GTP. In the presence of a non-hydrolyzable GTP analogue, guanosine 5′-(3-O-thio)triphosphate (GTPγS), isoproterenol increased cAMP formation to the same extent as that observed with AlF4?. Cholera toxin treatment of parotid membranes led to the ADP-ribosylation of two proteins (≈ 45 and 51 kDa). Cholera toxin also specifically decreased the high affinity GTPase activity in membranes and increased cAMP formation induced by GTP in the absence or the presence of isoproterenol. These data demonstrate that the high affinity GTPase characterized here is the ‘turn-off’ step for the adenylyl cyclase activation seen following β-adrenergic stimulation of rat parotid glands. 相似文献