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1.
Superoxide anion (O
2
.–
) was photogenerated upon illumination of riboflavin in fluorescent light. The rate of O
2
.–
formation was stimulated by double stranded DNA but not by denatured DNA or RNA. Depurinated DNA, which was predominantly depleted in guanine residues, did not exhibit the stimulatory effect, indicating an interaction of riboflavin, or active oxygen species derived from it, with guanine bases. Also, the stimulation of O
2
.–
photogeneration was not observed with ethidium bromide but was seen with proflavin-intercalated DNA. Since ethidium bromide intercalates preferentially between purines and pyrimidines, and proflavin prefers dA-dT rich sites, these results were interpreted to suggest that the interaction of riboflavin with DNA is mainly with GC or CG base pairs. 相似文献
2.
Cyclophosphamide (CYC) is a known chemotherapeutic drug used widely for the treatment of leukemias, lymphomas and some solid tumors. Copper is an essential constituent of chromatin and its level is usually elevated in various malignancies. Combined modality chemotherapy involves the use of drug with other components for cancer treatment, such as radiation therapy or surgery. Photosensitizer anticancer drugs can be used in combination with light and may have synergistic effect on cancer. The present study is an attempt to show that CYC acts as prooxidant when used in combination with Cu(II) and white light. We hypothesize that CYC when given as a chemotherapeutic agent possibly interact with endogenous copper associated with chromatin of the cancer cells and generate ROS besides acting as DNA alkylating agent. Thus, during chemotherapy the oxidative stress is possibly generated by the drug through mobilizing endogenous Cu(II) which may attribute to the cytotoxic death of cancer cell. 相似文献
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Aims
Quercetin is a natural polyphenolic flavonoid and acts as a quencher for reactive oxygen species generated by any physical or chemical action. In type 2 diabetes mellitus (T2DM) the basic characteristic feature is hyperglycemia which leads to complications involving oxidative stress. In view of this, the present study was conducted to examine the effect of quercetin in T2DM.Main methods
A total of 18 mice were divided into three groups, vis control, diabetic and diabetic treated with quercetin. Fasting blood glucose (FBG) levels and anti-oxidant enzyme activity were assayed. Creatinine, urea, lipid peroxidation, GLUT4 expression and DNA damage were also measured.Key findings
A significant decrease in FBG level and liver and kidney marker enzymes was observed in the quercetin treated group as compared to the diabetic one. Glutathione, SOD, catalase, and glutathione-S-transferase levels were also found to be increased on quercetin supplementation. Thiobarbituric acid-reactive substance level was decreased while GLUT4 expression levels were increased in the treated group. DNA damage was also affected positively by quercetin when subjected with single cell alkaline gel electrophoresis. Thus, we may suggest an anti-oxidant potential and protective effect of quercetin in T2DM mice.Significance
From this study, we conclude that quercetin ameliorates hyperglycemia and oxidative stress, by blunting free radical induced toxicity in T2DM. 相似文献5.
Wang Xia Khushk Imrana Xiao Yanqiu Gao Qiuqiang Bao Jie 《Applied microbiology and biotechnology》2018,102(1):377-388
Applied Microbiology and Biotechnology - Robustness of fermenting strains to lignocellulose derived inhibitors is critical for efficient biofuel and biochemical productions. In this study, the... 相似文献
6.
Zhou Pingping Khushk Imrana Gao Qiuqiang Bao Jie 《Journal of industrial microbiology & biotechnology》2019,46(7):951-963
Journal of Industrial Microbiology & Biotechnology - Furaldehydes and benzaldehydes are among the most toxic inhibitors from lignocellulose pretreatment on microbial growth and metabolism. The... 相似文献
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The effect of aminophylline on human red blood cells (RBC) has been studied. Under in vitro condition, aminophylline alone does not hemolyse RBC. However, in the presence of riboflavin and visible light, aminophylline causes hemolysis of RBC. This hemolysis depends on the concentration of both riboflavin and aminophylline. Using different free radical scavengers we show that RBC hemolysis is caused by reactive oxygen species. Studies using bovine serum albumin show that riboflavin-aminophylline combination can also cause protein degradation in vitro. 相似文献
9.
In an urbanized setting in Pakistan, 14 nightmare sufferers (NS) were compared with 14 control dreamers (CD) on the standard scales of the MMPI (Urdu) as well as on self-reported ratings of dream content and sleep problems. These subjects were selected on a volunteer basis. Although the average MMPI profile of both groups was within the normal range (between T scores of 40 and 60), the nightmare sufferers obtained significantly higher scores on 7 of 10 clinical scales; the largest differences were on the psychasthenia, paranoia, and schizophrenia scales. Results are interpreted in light of the Pakistani cultural context. 相似文献
10.
Asim Rizvi Mohd Farhan Imrana Naseem S. M. Hadi 《Apoptosis : an international journal on programmed cell death》2016,21(9):997-1007
Calcitriol is the metabolically active form of Vitamin D and is known to kill cancer cells. Using the rat model of DEN induced hepatocellular carcinoma we show that there is a marked increase in cellular levels of copper in hepatocellular carcinoma and that calcitriol–copper interaction leads to reactive oxygen species mediated DNA breakage selectively in hepatocellular carcinoma cells. In vivo studies show that calcitriol selectively induces severe fluctuations in cellular enzymatic and non enzymatic scavengers of reactive oxygen species in the malignant tissue. Lipid peroxidation, a well established marker of oxidative stress, was found to increase, and substantial cellular DNA breakage was observed. We propose that calcitriol is a proxidant in the cellular milieu of hepatocellular carcinoma cells, and this copper mediated prooxidant action of calcitriol causes selective DNA breakage in malignant cells, while sparing normal (non malignant) cells. 相似文献