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1.
Iliya I Ali Z Tanaka T Iinuma M Furusawa M Nakaya Ki Murata J Darnaedi D 《Phytochemistry》2002,61(8):959-961
An acetone extract of the stem of Gnetum latifolium Blume afforded the stilbene trimer (latifolol) together with five known stilbenoids (gnetin E, gnetin D, gnetin C, (-)epsilon -viniferin and resveratrol). Their structures were elucidated on the basis of spectral evidence, in particular by using 2D NMR methods. 相似文献
2.
Iliya G. Pashev 《Molecular biology reports》1977,3(4):297-303
Mouse DNA and chromatin were melted on hydroxyapatite and the denaturation profiles of ribosomal and satellite DNAs were followed by hybridization with their complementary RNAs. Neither ribosomal nor bulk DNA had significantly different melting profiles in chromatin as compared to DNA. However, most of satellite DNA eluted at higher temperature from chromatin than from purified DNA. One explanation for the higher melting temperature of mouse satellite DNA in chromatin suggests that the complex between this particular DNA component and at least some proteins in chromatin is more stable than the average DNA-protein interaction. 相似文献
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4.
Iva Ugrinova Stanislava Zlateva Iliya G. Pashev Evdokia A. Pasheva 《The international journal of biochemistry & cell biology》2009,41(7):1556-1562
The high mobility group box (HMGB) 1 protein, one of the most abundant nuclear non-histone proteins has been known for its inhibitory effect on repair of DNA damaged by the antitumor drug cisplatin. Here, we report the first results that link HMGB1 to repair of cisplatin-treated DNA at nucleosome level. Experiments were carried out with three types of reconstituted nucleosomes strongly positioned on the damaged DNA: linker DNA containing nucleosomes (centrally and end-positioned) and core particles. The highest repair synthesis was registered with end-positioned nucleosomes, two and three times more efficient than that with centrally positioned nucleosomes and core particles, respectively. HMGB1 inhibited repair of linker DNA containing nucleosomes more efficiently than that of core particles. Just the opposite was the effect of the in vivo acetylated HMGB1: stronger repair inhibition was obtained with core particles. No inhibition was observed with HMGB1 lacking the acidic tail. Binding of HMGB1 proteins to different nucleosomes was also analysed. HMGB1 bound preferentially to damage nucleosomes containing linker DNA, while the binding of the acetylated protein was linker independent. We show that both the repair of cisplatin-damaged nucleosomes and its inhibition by HMGB1 are nucleosome position-dependent events which are accomplished via the acidic tail and modulated by acetylation. 相似文献
5.
Stephen Saikiu Shaida Judith Sophie Weber Thaddeus Terlumun Gbem Sen Claudine Henriette Ngomtcho Usman Baba Musa Mbunkha Daniel Achukwi Mohammed Mamman Iliya Shehu Ndams Jonathan Andrew Nok Soerge Kelm 《BMC microbiology》2018,18(1):180
Background
Tsetse flies are vectors of trypanosomes, parasites that cause devastating disease in humans and livestock. In the course of vector control programmes it is necessary to know about the Glossina species present in the study area, the population dynamics and the genetic exchange between tsetse fly populations.Results
To achieve an overview of the tsetse fly diversity in Nigeria and at the Nigeria-Cameroon border, tsetse flies were trapped and collected between February and March 2014 and December 2016. Species diversity was determined morphologically and by analysis of Cytochrome C Oxidase SU1 (COI) gene sequences. Internal transcribed spacer-1 (ITS-1) sequences were compared to analyse variations within populations. The most dominant species were G. m. submorsitans, G. tachinoides and G. p. palpalis. In Yankari Game Reserve and Kainji Lake National Park, G. submorsitans and G. tachinoides were most frequent, whereas in Old Oyo National Park and Ijah Gwari G. p. palpalis was the dominant species. Interestingly, four unidentified species were recorded during the survey, for which no information on COI or ITS-1 sequences exists. G. p. palpalis populations showed a segregation in two clusters along the Cameroon-Nigerian border.Conclusions
The improved understanding of the tsetse populations in Nigeria will support decisions on the scale in which vector control is likely to be more effective. In order to understand in more detail how isolated these populations are, it is recommended that further studies on gene flow be carried out using other markers, including microsatellites.6.
Galina Yahubyan Mariyana Gozmanova Iliya Denev Valentina Toneva Ivan Minkov 《Plant Growth Regulation》2009,57(1):49-56
The relic endemic nature of Haberlea rhodopensis, which grows in Balkan Peninsula, in combination with its high vegetative desiccation-tolerance, makes this species a good
model to study mechanisms behind plant adaptation to severe drought stress. The aim of this study was to evaluate the antioxidant
protection provided by Superoxide dismutase (SOD) and Peroxidase (PO) in H. rhodopensis after exposure to and recovery from dehydration at different developmental stages. During dehydration the electrolyte leakage
from leaf tissue increased more significantly in post-flowering plants than in flowering plants, while upon subsequent rehydration
this parameter showed a very fast decrease to the basic value of fresh leaves and did not depend on developmental stage. Like
other higher plant species, SOD and PO demonstrated in H. rhodopensis an ability to adjust their activity very promptly to changing water supply. In addition, the leaves of this resurrection
species retained significant activities of SOD and PO even in air-dried state, considered as the most severe form of water
stress. The enhanced activity of antioxidant enzymes may either enable the scavenging of the active oxygen species produced
at very severe water deficit, and/or carry a potential for resurrection on subsequent rehydration. Upon stress treatment total
activities of both enzymes were higher in flowering than post-flowering plants which reveals that developmental stage might
be a factor affecting plant stress tolerance. This work identified for the first time SOD isoforms of H. rhodopensis. Native PAGE showed at least six multiple isoforms in the protein extract from leaf tissue of flowering plants, and the differential
visualization revealed that four of them were Cu, Zn-SOD isoforms, one was Mn-SOD and one Fe-SOD. These findings provide a
good starting point for future study of the SOD gene family of this rare resurrection plant at the molecular level. 相似文献
7.
Ignatova Milena Manolova Nevena Rashkov Ilia Vassileva Valya Ignatov George 《Plant and Soil》2000,227(1-2):27-34
The efficacy of Fe3+ complexes of polyethers with 8-quinolinol (8QOH) chelating groups attached to the polymer chain at different positions of
the aromatic ring or having different chain length for remedying the iron-deficient maize plants was evaluated. The efficacy
of chelates of polymers having terminal 8QOH residues was compared with that of complexes of ethylenediaminetetraacetic acid,
8QOH, mixtures of commercial polyethers with isopropylamino end-groups and 8QOH or FeCl3.6H2O. It was found that at 30/25 °C (day/night) and photosynthetic photon flux density 1100–1300 μmol m−2 s−1, the chlorotic maize plants recovered for 4 days of iron re-supply. An increase in the fresh and dry weight, leaf area, net
photosynthetic CO2 uptake of maize leaves, leaf pigment composition and chlorophyll fluorescence was more pronounced in the plants supplied
with Fe3+ chelates of polymers bearing 8QOH groups attached at 5-position, compared to the other tested Fe3+complexes. The importance of the stability of Fe3+ complexes, structure of the chelating agent and the necessity of effective ligand exchange between synthetic chelators and
free phytosiderophore in iron uptake by strategy II plants was discussed.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
8.
Front Cover: Ultraviolet radiation oxidative stress affects eye health (J. Biophotonics 7/2018) 下载免费PDF全文
Iliya V. Ivanov Timo Mappes Patrick Schaupp Christian Lappe Siegfried Wahl 《Journal of biophotonics》2018,11(7)
The role of ultraviolet radiation in oxidative stress‐related ocular pathologies is less known than its role in skin cancer. Excessive exposure to ultraviolet radiation is associated with increased oxidative stress in eye tissues, which may promote the development of photokeratitis, cataract, and retinal damages. Children are especially vulnerable: large pupils, transparent ocular media. Efficient everyday protection of the eye should be considered from early age. (Image: with permission from Carl Zeiss Vision International GmbH, Aalen, Germany) Further details can be found in the article by Iliya V. Ivanov, Timo Mappes, Patrick Schaupp, et al. ( e201700377 ).
9.
The denaturation of mouse satellite DNA upon melting of chromatin in solution of low ionic strength has been studied. A procedure for preparation of partially denaturated chromatin was developed which enabled the isolation of double-stranded (non-denatured) DNA sequences according to their thermal stability in chromatin. The content of mouse satellite DNA in these DNA sequences was determined by hybridization with RNA, complementary to satellite DNA in order to find the temperature interval of denaturation of satellite DNA. It was found that the melting temperature of satellite DNA in chromatin was lower than that of the total DNA. The results are discussed in relation to previously reported anomalous behaviour of satellite DNA upon melting of chromatin on hydroxyapatite. 相似文献
10.
High mobility group box (HMGB) proteins 1 and 2 are abundant non-histone nuclear proteins that regulate chromatin structure
because of their structure-specific binding to DNA. Here, we have investigated how the post-synthetic acetylation of HMGB1
affects its interaction with negatively supercoiled DNA by employing monoacetylated at Lys2 protein, isolated from butyrate-treated
cells. Our data reveal that this modification enhances three reaction parameters: binding affinity, supercoiling activity
and capacity to protect the supercoiled DNA from relaxation by topoisomerase I. We show that monoacetylation at Lys2 mimics
the effect of acidic tail removal but to a lesser extent thus demonstrating that in vivo acetylated HMGB1 is capable of modulating
its interaction with negatively supercoiled DNA. 相似文献