Changes of localization of lectin-binding sites on the intestinal epithelial cell surface membrane during differentiation of the columnar intestinal cells

Light and electron microscopically localizations of Concanavalin A, Soybean Agglutinin, and Asparagus Pea Lectin-binding sites on the intestinal microvillous membrane were studied by means of horseradish peroxidase labelled lectins. The distribution of the lectin-binding sites was composed with the cell differentiation from the crypts to the tip of the intestinal villi. The visualization of lectin receptors in the mucin vesicles in the goblet cells was established as well. The controls for the determination of the specificity of reactions were carried out.     

Demonstration of negative tissue charges by means of polyethyleneimine-metal complexes

Three polyethyleneimine-metal complexes were synthesized and cytochemically tested for demonstration of negatively charged sites. For this purpose rat cerebral cortex synaptosomes were used. It was established that both types of polyethyleneimine-copper complexes labeled the synaptosomal membrane and synaptic vesicles with electron-dense granules, whereas the polyethyleneimine-lead complex marked the anionic sites with amorphous electron-dense material.     

Demonstration of negative tissue charges by means of polyethyleneimine-metal complexes

Summary Three polyethyleneimine-metal complexes were synthesized and cytochemically tested for demonstration of negatively charged sites. For this purpose rat cerebral cortex synaptosomes were used. It was established that both types of polyethyleneimine-copper complexes labeled the synaptosomal embrane and synaptic vesicles with electrondense granules, whereas the polyethyleneimine-lead complex marked the anionic sites with amorphous electrondense material.Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday     

Soybean agglutinin-binding sites on the human sperm surface

Some differences in the distribution of lectin-binding sites on the surface of the plasma membrane of acrosomal and postacrosomal regions and tail of ejaculated human spermatozoa were demonstrated by means of horseradish peroxidase labeled soybean agglutinin. The acrosomal region was more densely coated with labeled lectin in comparison with the postacrosomal region. Hapten inhibition with N-acetyl-D-galactosamine was used as control.     

Demonstration of electron-dense material in clear synaptic vesicles using cationic ferrocenyl compounds

Summary Electron-dense material in clear synaptic vesicles in rat cerebral cortex and neuromuscular junctions of frog cutaneous pectoris muscle was demonstrated by using ferrocenyl cationics. Electron-dense spots were usually attached to the inner surface of the vesicular membrane. Control experiments (treatment with Triton X-100 or cetylpyridinium chloride; enzyme digestion with trypsin, hyaluronidase, neuraminidase, sulfatase and -glucuronidase) suggested that the electron-dense material is a glycoprotein.