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排序方式: 共有569条查询结果,搜索用时 15 毫秒
1.
M. I. Ibragimova A. I. Chushnikov G. V. Cherepnev V. Yu. Petukhov E. P. Zheglov 《Biophysics》2014,59(3):425-430
The iron metabolism was studied in serum blood samples collected from 26 professional sportsmen undergoing intensive physical exercises using EPR combined with hematological and biochemical laboratory tests. Only 23% of EPR spectra (n = 6) were practically normal while in the rest spectra additional abnormal absorption lines were detected. Presumably, the significant portion of new signals may be caused by different cytochromes. Moreover, the anisotropic signals with g 1 ? 2.02; g 2 ? 1.94 and g 3 ? 1.86 registered in some spectra pointed to the sulfur-iron centers. There was nearly linear correlation between the concentration of Fe3+ in transferrin (Fe3+-Tf) obtained from the EPR spectra and the serum iron concentration measured by absorption photometry both for sportsmen and controls (healthy individuals and patients with different diseases). At equal serum iron concentrations the Fe3+-Tf level was higher in sportsmen than that in controls. The Pearson correlation coefficient (r) for Fe3+-Tf and serum iron values was equal to 0.89 in sportsmen versus r = 0.97 in controls. Additional new lines in serum EPR spectra of professional sportsmen prove the suitability of EPR assay for scheduled medical exams since routine biochemical and hematological tests are insufficient to discover all abnormalities in iron metabolism under intensive physical exercises. 相似文献
2.
Genetic and demographic studies of urban sample of Turkmen-teke (Ashkhabad city) were performed among probands-36 patients with diagnosis of "schizophrenia" and their 840 relatives. Clinical analysis revealed 27.8% of phenocopies. In other 26 families the genealogical analysis showed autosomal-dominant (12 families), probably, autosomal-dominant (9 families) as well as autosomal-recessive (3 families) types of inheritance of pathology, in two families the type being not established. The empiric risk of affection was determined to be 24.2% for parents, 75.5% for sibs and 33.3% for children. Mean number of children per one woman is 3.53, which is significantly lower than mean populational number (6.17). High level of endogamy of the urban sample tested is established, the total coefficient of inbreeding being 0.009856; grandparents of the probands appeared to be exclusively of rural origin. The negative action of selection (decreased fitness), preferentially monogenic type of heredity and high level of endogamy are discussed as factors providing broad hereditary heterogeneity of schizophrenia. 相似文献
3.
司光伟 《生物化学与生物物理进展》2023,50(6):1243-1243
Acetylcholine, the first identified neurotransmitter, plays crucial roles in various brain functions. One well-known case is its involvement as an activating neurotransmitter in the regulation of locomotion. However, its inhibitory regulatory role, particularly in locomotion, remains poorly understood. In a study conducted by Polat et al., the authors investigated the inhibitory role of acetylcholine in locomotion in C. elegans. In this organism, the acetylcholine-gated chloride channel receptor consists of four subunits. The authors thoroughly examined the loss-of-function of each subunit in movement regulation. Interestingly, the mutant worms were still capable of performing various movements such as forward, backward crawling, and turning, suggesting that the overall movement was not significantly affected. However, quantitative behavior analysis revealed subtle yet significant differences in the timing and postures of the movement in these mutants. Furthermore, the authors employed optogenetics to stimulate a specific neuron involved in backward crawling and demonstrated that the loss-of-function of the receptors in individual neurons affects the transitioning between locomotion modes.
This work provides evidence for the inhibitory regulatory role of acetylcholine in locomotion. The loss-of-function of acetylcholine-gated chloride channel receptors likely disrupts the balance of neuronal and circuit physiology, thereby affecting the regulation of locomotion. Moreover, this study highlights the powerful role of quantitative behavior analysis in discovering and understanding more sophisticated functions of neural circuits. 相似文献
4.
Ibragimova SI Kozlov DG Kartasheva NN Sutsov NI Efremov BD Benevolensky SV 《Biotechnology and bioengineering》1995,46(3):285-290
A procedure was developed for construction of industrial strains of distiller's yeast (Saccharomyces cerevisiae). It includes several steps: construction of congenic genetically marked haploid strains of opposite mating types starting from an industrial strain of hybrid nature, integrative transformation of the above haploid strains with a DNA fragment containing an expression cassette responsible for new technological facilities, and hybridization of transformants and isolation of final industrial homozygous strains under experimental conditions simulating commercial fermentation processes. This strategy permits the generation of strains that have desirable characteristics of traditional races of distiller's yeast along with new technological facilities determined by the particular expression cassette. Using this procedure, we have constructed an industrial strain with improved amylolytic activity. (c) 1995 John Wiley & Sons, Inc. 相似文献
5.
为筛选铁皮石斛(Dendrobiumofficinale)花总RNA提取方法,对8种提取方法进行了比较研究,包括改良CTAB-LiCl法(M1)、改良CTAB-异丙醇法(M2)、改良SDS-LiCl法(M3)、改良SDS-异丙醇法(M4)、多糖多酚植物RNA提取试剂盒法(M5)、柱式植物RNAout 2.0试剂盒法(M6)、RNAprep Pure多糖多酚植物总RNA提取试剂盒法(M7)和Biospin多糖多酚植物总RNA提取试剂盒法(M8)。结果表明,以M4和M5提取的总RNA带型清晰,完整性好,A260 nm/A280 nm为1.8~2.0,A260 nm/A230 nm大于2.0,RNA产率分别为(159.45±1.45)和(170.84±3.53)μg/g。利用M4、M5提取霍山石斛、金钗石斛、鼓槌石斛和美花石斛花的总RNA,样品的完整性、浓度和纯度均符合质量要求。以M4、M5提取的铁皮石斛总RNA为模板,扩增Actin基因片段,扩增产物大小与预期一致且条带单一。这说明M4、M5方法操作简便,结果重复性好,能够较好地提取石斛属植物花的总RNA。 相似文献
6.
Biophysics - This study focused on the redox homeostasis in the lenses of 20- and 30-day-old chinchilla rabbit kits exposed to a combination of hypoxia and electromagnetic radiation at a nonthermal... 相似文献
7.
急性出血性结膜炎(Acute hemorrhagic conjunctivitis,AHC)是目前人类最常见的眼病之一,柯萨奇病毒A组24型变异株(Coxsackievirus A24 variant,CV-A24v)是近年来报道引起该病的主要病原体。本研究选取10株来自江西省2010年AHC暴发疫情的CV-A24v,采用特异性引物扩增并测定其全基因组序列。对该10条CV-A24v的全基因组序列进行系统发育分析以及重组分析,计算本研究测定的江西10条以及GenBank中所有22条CV-A24v的全基因组序列的氨基酸置换熵值,并预测其正向选择位点。结果表明,在江西10条CV-A24v基因组序列中未检测到重组。基于全基因组序列构建的最大似然树表明江西10株CV-A24v属于GIV基因型,且分处于两条传播链。对上述32条CV-A24v序列的氨基酸置换熵值计算,共得到25个易突变位点(熵值>0.6),易突变概率最高的区段为2A区。基于Datamonkey中FUBAR和FEL模型分析,发现位于结构蛋白VP2区的234位氨基酸为两种模型共同获得的CV-A24v的正向选择位点。本研究分析了江西10株CV-A24v的全基因组序列特征,为CV-A24v引起的AHC防控工作提供了基础资料。 相似文献
8.
[目的]为明确不同幼虫密度和不同温度条件对水芹叶象甲Hypera sp.未成熟阶段生长发育的影响.[方法]在室内条件下对不同幼虫饲养密度和不同温度条件饲养的水芹叶象甲未成熟阶段(卵、幼虫、预蛹和蛹)的存活和生长发育进行研究.[结果]幼虫密度对水芹叶象甲幼虫、预蛹和蛹的存活和生长发育均具有显著影响.水芹叶象甲幼虫、预蛹和蛹在50头/盒的密度条件下的存活率均显著高于其它密度条件,且发育历期显著短于其它密度条件.此外,各密度条件下的雌蛹重均显著重于雄蛹重,但性比(♀/♂)不受密度条件的影响.温度条件对水芹叶象甲卵、幼虫、预蛹和蛹的存活和生长发育亦均具有显著影响.27℃条件下的幼虫和蛹的存活率均显著高于其它温度条件,1龄幼虫在30℃条件下的存活率显著高于其它温度条件,而3龄幼虫在27℃条件下的存活率显著高于其它温度条件,但温度条件对2龄幼虫的存活率无显著影响.水芹叶象甲各龄期幼虫及各虫态的发育历期随着温度升高而显著缩短.[结论]幼虫饲养密度和温度是影响水芹叶象甲种群变化的重要因子,这将为分析水芹叶象甲种群动态变化规律,以 及提高该虫的预测预报和综合防控水平提供依据. 相似文献
9.
Recent sequencing studies of clear cell (conventional) renal cell carcinoma (ccRCC) have identified inactivating point mutations in the chromatin-modifying genes PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2 and BAP1. To investigate whether aberrant hypermethylation is a mechanism of inactivation of these tumor suppressor genes in ccRCC, we sequenced the promoter region within a bona fide CpG island of PBRM1, KDM6A, SETD2 and BAP1 in bisulfite-modified DNA of a representative series of 50 primary ccRCC, 4 normal renal parenchyma specimens and 5 RCC cell lines. We also interrogated the promoter methylation status of KDM5C and ARID1A in the Cancer Genome Atlas (TCGA) ccRCC Infinium data set. PBRM1, KDM6A, SETD2 and BAP1 were unmethylated in all tumor and normal specimens. KDM5C and ARID1A were unmethylated in the TCGA 219 ccRCC and 119 adjacent normal specimens. Aberrant promoter hypermethylation of PBRM1, BAP1 and the other chromatin-modifying genes examined here is therefore absent or rare in ccRCC. 相似文献
10.
Ghukasyan L. G. Krasnov G. S. Muravenko O. V. Ikonnikova A. Y. Yurasov R. A. Baidun L. V. Ibragimova S. Z. Nasedkina T. V. 《Molecular Biology》2020,54(3):341-348
Molecular Biology - Certain subtypes of acute myeloid leukemia occur as a result of the cooperation of several events these are, the formation of fusion genes as a result of chromosomal... 相似文献