全文获取类型
收费全文 | 342篇 |
免费 | 7篇 |
专业分类
349篇 |
出版年
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 3篇 |
2014年 | 1篇 |
2013年 | 3篇 |
2012年 | 8篇 |
2011年 | 9篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2007年 | 11篇 |
2006年 | 16篇 |
2005年 | 10篇 |
2004年 | 9篇 |
2003年 | 13篇 |
2002年 | 10篇 |
2001年 | 13篇 |
2000年 | 9篇 |
1999年 | 18篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 7篇 |
1987年 | 7篇 |
1986年 | 7篇 |
1985年 | 6篇 |
1984年 | 6篇 |
1983年 | 7篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 8篇 |
1978年 | 8篇 |
1977年 | 4篇 |
1976年 | 6篇 |
1975年 | 9篇 |
1974年 | 11篇 |
1973年 | 10篇 |
1972年 | 11篇 |
1971年 | 9篇 |
1970年 | 7篇 |
1969年 | 8篇 |
1968年 | 7篇 |
1967年 | 3篇 |
1966年 | 9篇 |
1965年 | 4篇 |
排序方式: 共有349条查询结果,搜索用时 15 毫秒
1.
2.
3.
Basic principles have been developed for a discrete stochastic simulation model of an elementary proliferative unit of the intestinal epithelium, a "crypt-villus" system. The analysis of the results obtained after a single exposure of the animal's abdomen to 3 and 6 Gy radiation has demonstrated that the dynamics of the number of cells that synthesize DNA in a small intestine crypt of exposed mice depends on the rate of radiation damage repair (50 to 100 h following irradiation). The rate of repair after 6 Gy irradiation is 1.5 times lower that after 3 Gy. The changes in the shape of the labeled mitoses curve, followed up during the postirradiation recovery of the intestinal epithelium, may occur with the time parameters of the cell mitotic cycle being invariable. 相似文献
4.
V P Iakovlev L A Blatun E P Khlebnikov R V Makarenkova L V Elagina L S Puchkova E V Kutsidi 《Antibiotiki i khimioterapii͡a》1991,36(9):44-46
A clinico-laboratory study on ciprofloxacin made by Bayer (Germany) was applied to patients with extended posttraumatic wounds and performed with the aim of preventing postoperative purulent complications in patients operated on the organs of the gastrointestinal tract. In the both groups ciprofloxacin was administered orally in doses of 500 and 1000 mg and intravenously in a dose of 200 mg. The results of the assay on ciprofloxacin sensitivity of the isolates from the wound excretion and urine showed that they were more sensitive to ciprofloxacin than to aminoglycosides and cephalosporins. 15 minutes after the intravenous administration the serum concentration of ciprofloxacin amounted to 7.5 +/- 0.9 micrograms/ml and in 6 hours it was equal to 0.45 +/- 0.45 micrograms/ml, the mean concentrations of ciprofloxacin being attained in the bile (8.7 +/- +/- 3.9 micrograms/ml), gallbladder wall (5.5 +/- 3.8 micrograms/g), liver (0.73 micrograms/g), muscles (1.93 micrograms/g) and tendon (0.15 microgram/g). After the oral administration in a dose of 500 mg ciprofloxacin was detected in the blood serum in an amount of 2.0 +/- 0.7 micrograms/ml in 1 hour and in an amount of 0.9 +/- 0.13 micrograms/ml in 6 hours. After the drug oral administration in a dose of 1000 mg the maximum concentrations were: 6.34 +/- 4.2 micrograms/ml on the average and 2.1 +/- 0.8 micrograms/ml in 6 hours (0.4 micrograms/g in the muscles, 1.4 micrograms/g in the skin and 0.34 micrograms/g in the bones). The study showed that ciprofloxacin was a highly efficient antimicrobial agent in the treatment of the complicated wound infections and the prophylaxis of the purulent complications during the postoperative period in the patients operated on gastrointestinal organs. 相似文献
5.
6.
7.
8.
D A Perumov V V Machkovski? D Iu Iakovlev E I Budovski? 《Bioorganicheskaia khimiia》1984,10(12):1695-1697
The action of methoxyamine (MA) on B. subtilis transforming DNA (50 degrees C, pH 4,5 and 6,0, 1 M MA) was studied. The rate of cytosine residues modification in DNA is 250 times less than in monomer (rate constants for DNA are 1,5 X 10(-1) min-1 at pH 4,5, and 2,5 X 10(-6) min-1 in the first 300 hours of treatment at pH 6,0). At pH 4,5 the rates of cytosine (I) conversion into N4-methoxycytosine (II) and into 6-methoxyamino-5,6-dihydro-N4-methoxycytosine (III) are constant (II/III ratio is about 2,1). At pH 6,0 the II/III ratio smoothly increases from 1,0 to 1,6 (200 and 900 hours of treatment) due to a decrease in the product III accumulation rate. The frequency of MA-induced mutations shows a bell-shaped dependence on time with maxima (approximately 10%) at 80 (pH 4,5) and 500 (pH 6,0) hours of treatment. In both cases approximately 10% of cytosine residues are modified. These results suggest that either compound III is efficiently removed from the transforming DNA, or its presence does not arrest the DNA replication. 相似文献
9.
Iris M Costa Tallybia HT Nasser Marilene Demasi Rafaella MP Nascimento Luis ES Netto Sayuri Miyamoto Fernanda M Prado Gisele Monteiro 《BMC microbiology》2011,11(1):268
Background
The gene YCL047C, which has been renamed promoter of filamentation gene (POF1), has recently been described as a cell component involved in yeast filamentous growth. The objective of this work is to understand the molecular and biological function of this gene.Results
Here, we report that the protein encoded by the POF1 gene, Pof1p, is an ATPase that may be part of the Saccharomyces cerevisiae protein quality control pathway. According to the results, Δpof1 cells showed increased sensitivity to hydrogen peroxide, tert-butyl hydroperoxide, heat shock and protein unfolding agents, such as dithiothreitol and tunicamycin. Besides, the overexpression of POF1 suppressed the sensitivity of Δpct1, a strain that lacks a gene that encodes a phosphocholine cytidylyltransferase, to heat shock. In vitro analysis showed, however, that the purified Pof1p enzyme had no cytidylyltransferase activity but does have ATPase activity, with catalytic efficiency comparable to other ATPases involved in endoplasmic reticulum-associated degradation of proteins (ERAD). Supporting these findings, co-immunoprecipitation experiments showed a physical interaction between Pof1p and Ubc7p (an ubiquitin conjugating enzyme) in vivo.Conclusions
Taken together, the results strongly suggest that the biological function of Pof1p is related to the regulation of protein degradation.10.