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Effect of Exogenous Nitrate on Anaerobic Metabolism in Excised Rice Roots: I. NITRATE REDUCTION AND PYRIDINE NUCLEOTIDE POOLS 总被引:1,自引:0,他引:1
Reggiani, R., Brambilla, I. and Bertani, A. 1985. Effect ofexogenous nitrate on anaerobic metabolism in excised rice roots.I. Nitrate reduction and pyridine nucleotide pools. J.exp.Bot 36:1193-1199. In apical segments of sterile rice roots, reduction of nitratein the absence of oxygen is promoted by the presence of exogenousnitrate in the growth medium in the first 3 h of oxygen deficiency.Anaerobic treatment also increased the concentration of NADH.When nitrate was made available for reduction, smaller accumulationsof NADH were observed. Oxidation of reduced nudeotides associatedwith the conversion of nitrate to nitrite is suggested as beingresponsible for this behaviour. Key words: Anaerobiosis, nitrate, pyridine nucleotides 相似文献
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GÖRAN E. NILSSON NATALIE CRAWLEY†‡ IDA G. LUNDE PHILIP L. MUNDAY†‡ 《Global Change Biology》2009,15(6):1405-1412
The capacity for marine fishes to perform aerobically (aerobic scope) is predicted to control their thermal tolerance and, thus, the impact that rapid climate change will have on their populations. We tested the effect of increased water temperatures on the resting and maximum rates of oxygen consumption in five common coral reef fishes at Lizard Island on the northern Great Barrier Reef, Australia. All species exhibited a decline in aerobic capacity at elevated water temperatures (31, 32 or 33 °C) compared with controls (29 °C); however, the response was much stronger in two cardinalfishes, Ostorhinchus cyanosoma and O. doederleini , compared with three damselfishes, Dascyllus anuarus, Chromis atripectoralis and Acanthochromis polyacanthus . Aerobic scope of the two cardinalfishes was reduced by nearly half at 31 °C compared with 29 °C, and virtually all capacity for additional oxygen uptake was exhausted by 33 °C. In contrast, the three damselfishes retained over half their aerobic scope at 33 °C. Such differences in thermal tolerance between species, and possibly families, suggest that the community structure of reef fish assemblages might change significantly as ocean temperatures increase. Populations of thermally tolerant species are likely to persist at higher temperatures, but populations of thermally sensitive species could decline on low-latitude reefs if individual performance falls below levels needed to sustain viable populations. 相似文献
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A Comparative Investigation of the Bactericidal and Fungicidal Effects of Three Phenolic Disinfectants 总被引:2,自引:2,他引:0
IDA K. HEGNA 《Journal of applied microbiology》1977,43(2):177-181
Two methods, a capacity use-dilution test and another employing geometrical dilution, are used when comparing the bactericidal and fungicidal effects of a phenolic disinfectant containing 45% (w/w) o -phenylphenol in an aqueous soap solution of linseed oil (soap content 6·5% w/v) with the corresponding effects of the same phenolic compound in an aqueous soya oil soap solution (soap content 3·5% w/v). The soya oil soap did not change the disinfectant capacity on the different test organisms used. For the most resistant strain ( Staphylococcus aureus ) the usedilution concentration was evaluated to be slightly above 1%, i.e. 2%, which is much lower than the recommended use-dilution concentration. However, using the same method and test organisms the capacity use-dilution testing of a third phenolic disinfectant, containing p -chloro- m -cresol and o -benzyl- p -chlorophenol with a total of 9·2 (w/w) phenols in a detergent system, indicated that the recommended use-dilution concentration should be doubled. 相似文献
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The effect of sewage effluent on denitrification in Grasmere (English Lake District) 总被引:1,自引:0,他引:1
SUMMARY. Denitrification processes in Grasmere were studied using several techniques. Sediments were analysed for nitrate reductase activity and the accumulation of nitrogenous gases in samples incubated in the laboratory. Changes in the concentrations of inorganic nitrogen species in the water column and the release of gas bubbles into gas traps suspended above the sediment were also measured. Sewage effluent entered the lake via the main inflow and faecal bacteria were used as tracers to map the path of the river (and effluent) across the lake. The main river flow was west of the island in the centre of the lake and across the smaller of the two main basins. A preliminary study indicated that this western basin was the site of most active denitrification and this was confirmed in the following year when a seasonal survey showed that deoxygenation, nitrate reduction and ammonium accumulation was more advanced at this site. In addition, this basin was the only area where consistent discharge of nitrogen and methane from the profundal zone was observed. The nitrate reductase assay demonstrated that the sediment was the major site of denitrification, and that activity in the water column was higher in the anoxic hypolimnion than in the euphotic zone. Gas evolution from incubated sediment samples was analysed by gas liquid chromatography (GLC) and indicated that maximum denitrifying activity occurred below the mud surface in oxidized sediments. There was reasonable agreement between the enzyme assay and the GLC technique but neither predicted events in the field satisfactorily. Viable, most probable number (MPN) counts of denitrifying bacteria had similar limitations. A bimodal seasonal pattern of nitrate reductase activity was observed at four sites during thermal stratification, but this could not be explained fully. The major site of denitrification was identified and it was calculated that the nitrogen gas discharged was equivalent to c. 54% of the nitrate removed from the water column. 相似文献
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Aphid‐tending ants on introduced fennel: can resources derived from non‐native plants alter the trophic position of higher‐order consumers?
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1. Although plant invasions often reduce insect abundance and diversity, non‐native plants that support phytophagous insects can subsidise higher trophic levels via elevated herbivore abundance. 2. Here ant–aphid interactions on non‐native fennel on Santa Cruz Island, California are examined. Fennel hosts abundant, honeydew‐producing fennel aphids. The patchiness of fennel and the relative lack of honeydew‐producing insects on other plants at our study sites suggest that assimilation of fennel‐derived honeydew would increase the abundance and decrease the trophic position of the omnivorous, aphid‐tending Argentine ant. 3. To assess the strength of the ant–aphid interaction, a comparison of ant abundance on and adjacent to fennel prior to and 3 weeks after experimental aphid removal was performed. Compared with control plants with aphids, ants declined in abundance on and around fennel plants following aphid removal. At the habitat scale, pitfall traps in fennel‐dominated habitats captured more ants than in fennel‐free scrub habitats. 4. To determine if assimilation of aphid‐produced honeydew reduces the ant's trophic position, variation in δ15N values among ants, plants and other arthropods was analysed. Unexpectedly, δ15N values for ants in fennel‐dominated habitats were higher than those of arthropod predators from the same sites and also higher than those of ants from fennel‐free habitats. 5. Our results illustrate how introduced plants that support phytophagous insects appear to transfer energy to higher trophic levels via elevated herbivore abundance. Although assimilation of fennel‐derived honeydew did not appear to reduce consumer trophic position, spatial variation in alternative food resources might obscure contributions from honeydew. 相似文献
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