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Holfgrefe Simone; Backhausen Jan E.; Kitzmann Camillo; Scheibe Renate 《Plant & cell physiology》1997,38(11):1207-1216
The reactions of isolated intact spinach chloroplasts at saturatinglight and CO2 to changes in steady-state electron flow werefollowed at the various stages of photosynthesis. Alterationsin the rate of electron flow were induced by the addition ofoxaloacetate (OAA), nitrite or methyl viologen (MV). Two typesof effect can be distinguished: (1) When a small fraction ofthe electrons produced are accepted by OAA or nitrite (up to20% of the electrons produced in the light), the activationstate of the NADP+-dependent malate dehydrogenase (NADP-MDH)was strongly decreased, whereas qP and the rate of O2-productionwere increased. qN, the stromal metabolite pools and the [14C]-CO2-fixationrate were only marginally influenced. (2) Higher amounts ofnitrite or MV decreased O2 production and strongly inhibited[14C]CO2 fixation. This treatment further increased the ATP/ADPratio, but had little effect on the NADPH + H+/NADP+ ratio.The stromal concentrations of 3PGA, DHAP and FBP, and the ratesof 3PGA and DHAP export were drastically changed. In particular,the DHAP/3PGA ratio increased, and the rate of 3PGA export wasdecreased by minor changes in the rate of electron flow. Additionof high amounts of nitrite or MV, but not of OAA decreased theactivation states of NADP-MDH and fructose 1,6-bisphosphatase(FBPase), while the activation states of NADP+-dependent glyceraldehyde3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK)remained unchanged under all conditions. (Received February 10, 1997; Accepted September 2, 1997) 相似文献
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