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J. Trémouillaux-Guiller H. Kodja J. C. Chénieux 《Plant Cell, Tissue and Organ Culture》1994,37(1):25-30
Protoplasts enzymatically isolated from cell line of Catharanthus roseus G. Don crown gall, were cultured at high density (105 P ml-1) in modified B5 liquid medium (Gamborg et al. 1976). In the absence of growth regulators C. roseus protoplasts were able to regenerate a cell-wall, divide and, subsequently, yield very numerous clones in the absence of growth regulators. After two weeks, the cultures were greatly diluted in order to obtain clones of single-cell origin. Most of the clones individually transferred onto solid medium can proliferate indefinitely, without growth regulators. Among analyzed clones, 90% were nopaline positive. Their ajmalicine and serpentine content was compared with that of the parental crown gall line, and was found to be low. The CR10 protoplasts were very easy to grow, they were an interesting model for the development of pure tumorous lines. Moreover, we found that the tumorous protoplasts were useful for cell fusion experiments or for the delicate culture of tree protoplasts.Abbreviations B5
Gamborg et al. (1976) medium
- 2,4-d
2,4-dichlorophenoxyacetic acid
- Kin
Kinetin
- NAA
naphthalene acetic acid
- BA
N6 (benzyl) adenine 相似文献
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Hritier Milenge Kamalebo Hippolyte Nshimba Seya Wa Malale Cephas Masumbuko Ndabaga Lon Nsharwasi Nabahungu Jrme Degreef Andr De KeseL 《African Journal of Ecology》2019,57(2):247-259
Ectomycorrhizal fungi constitute an important component of forest ecosystems that enhances plant nutrition and resistance against stresses. Diversity of ectomycorrhizal (EcM) fungi is, however, affected by host plant diversity and soil heterogeneity. This study provides information about the influence of host plants and soil resources on the diversity of ectomycorrhizal fungal fruiting bodies from rainforests of the Democratic Republic of the Congo. Based on the presence of fungal fruiting bodies, significant differences in the number of ectomycorrhizal fungi species existed between forest stand types (p < 0.001). The most ectomycorrhizal species‐rich forest was the Gilbertiodendron dewevrei‐dominated forest (61 species). Of all 93 species of ectomycorrhizal fungi, 19 demonstrated a significant indicator value for particular forest stand types. Of all analysed edaphic factors, the percentage of silt particles was the most important parameter influencing EcM fungi host plant tree distribution. Both host trees and edaphic factors strongly affected the distribution and diversity of EcM fungi. EcM fungi may have developed differently their ability to successfully colonise root systems in relation to the availability of nutrients. 相似文献
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Komguem J Meli AL Manfouo RN Lontsi D Ngounou FN Kuete V Kamdem HW Tane P Ngadjui BT Sondengam BL Connolly JD 《Phytochemistry》2005,66(14):1713-1717
Two new xanthones, smeathxanthone A (1) (2-(3,7-dimethyl-2,6-octadienyl)-1,3,5,8-tetrahydroxyxanthone) and smeathxanthone B (2) (5,7,10-trihydroxy-2-methyl-2-(4-methylpent-3-enyl)[2H, 6H]pyrano[3,2-b]xanthen-6-one), have been isolated from the stem bark of Garcinia smeathmannii, and their structures elucidated on the basis of 1D and 2D NMR experiments. 1,3,5-Trihydroxyxanthone and 1,5-dihydroxyxanthone were also obtained. The compounds showed only modest activity against a range of bacteria and yeasts. 相似文献
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James D. Simo Mpetga Mathieu Tene Hippolyte K. Wabo Shi-Fei Li Ling-Mei Kong Hong-Ping He Xiao-Jiang Hao Pierre Tane 《Phytochemistry letters》2012,5(1):183-187
Two new cycloartane-type triterpenoids, glaucartanoic acids A (1) and B (2), together with five known compounds were isolated from the fruits of Caloncoba glauca. Their structures were elucidated by spectroscopic methods including extensive 1D and 2D NMR techniques, by chemical evidence and by comparison with literature data. The new compounds were evaluated for their in vitro cytotoxicity against five human cancer cell lines. 相似文献
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Arno R.N. Donfack Ngeh J. Toyang Hippolyte K. Wabo Pierre Tane Maurice D. Awouafack Haruhisa Kikuchi Jean D.D. Tamokou Jules R. Kuiate Yoshiteru Oshima 《Phytochemistry letters》2012,5(3):596-599
Chemical investigation of the roots of Vernonia guineensis (Asteraceae) afforded a new stigmastane derivative, vernoguinoside A (1) and the known vernoguinoside (2), stigmasterol 3-O-β-d-glucoside (3) and sitosterol 3-O-β-d-glucoside (4). Their structures were elucidated by spectroscopic analysis. Antimicrobial activities of 1–3 and CH2Cl2–MeOH (1:1) extract were evaluated against three bacteria species (Salmonella typhi, Staphylococcus aureus and Shigella flexneri) and three yeasts species (Candida albicans, Candida parapsilosis and Cryptococcus neoformans). Compounds 1 and 2 exhibited both antibacterial and antifungal activities that varied between the microbial species (MIC = 7.81–125 μg/mL) while S. flexneri and C. albicans were sensitive to all the tested compounds. 相似文献
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Social network characteristics associated with weight loss among black and hispanic adults 下载免费PDF全文
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Hippolyte K. Wabo Haruhisa Kikuchi Yasuhiro Katou Pierre Tane Yoshiteru Oshima 《Phytochemistry letters》2010,3(2):104-107
A new xanthone, 3,4-dihydro-8,10,12-trihydroxy-2,2-dimethylpyrano[2,3-b]xanthen-11(2H)-one or butyraxanthone E (1), along with the known compounds 30-epi-cambogin (2), 1,7-dihydroxyxanthone (3) and 1,5-dihydroxyxanthone (4) were isolated from the roots of Pentadesma butyracea. Their structures were elucidated by spectroscopic means and comparison with published data. Their antiproliferative activities were evaluated against Drosophila S2 cells and two human cancer cell lines, THP-1 (leukemia) and HCT116 (colon cancer). Compounds 1 and 2 showed moderate antiproliferative activity against Drosophila S2 cells and the HCT116 cell line, respectively. Compound 2 was active against Drosophila S2 cells. 相似文献
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Ange-Marie Risterucci Isabelle Hippolyte Xavier Perrier Ling Xia Vanessa Caig Margaret Evers Eric Huttner Andrzej Kilian Jean-Christophe Glaszmann 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(6):1093-1103
Diversity Arrays Technology (DArT) is a DNA hybridisation-based molecular marker technique that can detect simultaneously
variation at numerous genomic loci without sequence information. This efficiency makes it a potential tool for a quick and
powerful assessment of the structure of germplasm collections. This article demonstrates the usefulness of DArT markers for
genetic diversity analyses of Musa spp. genotypes. We developed four complexity reduction methods to generate DArT genomic representations and we tested their
performance using 48 reference Musa genotypes. For these four complexity reduction methods, DArT markers displayed high polymorphism information content. We
selected the two methods which generated the most polymorphic genomic representations (PstI/BstNI 16.8%, PstI/TaqI 16.1%) to analyze a panel of 168 Musa genotypes from two of the most important field collections of Musa in the world: Cirad (Neufchateau, Guadeloupe), and IITA (Ibadan, Nigeria). Since most edible cultivars are derived from two
wild species, Musa acuminata (A genome) and Musa balbisiana (B genome), the study is restricted mostly to accessions of these two species and those derived from them. The genomic origin
of the markers can help resolving the pedigree of valuable genotypes of unknown origin. A total of 836 markers were identified
and used for genotyping. Ten percent of them were specific to the A genome and enabled targeting this genome portion in relatedness
analysis among diverse ploidy constitutions. DArT markers revealed genetic relationships among Musa genotype consistent with those provided by the other markers technologies, but at a significantly higher resolution and speed
and reduced cost. 相似文献