全文获取类型
收费全文 | 688篇 |
免费 | 47篇 |
专业分类
735篇 |
出版年
2023年 | 3篇 |
2022年 | 5篇 |
2021年 | 14篇 |
2020年 | 7篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 17篇 |
2015年 | 32篇 |
2014年 | 34篇 |
2013年 | 34篇 |
2012年 | 43篇 |
2011年 | 39篇 |
2010年 | 30篇 |
2009年 | 22篇 |
2008年 | 42篇 |
2007年 | 49篇 |
2006年 | 30篇 |
2005年 | 31篇 |
2004年 | 39篇 |
2003年 | 35篇 |
2002年 | 36篇 |
2001年 | 7篇 |
2000年 | 11篇 |
1999年 | 12篇 |
1998年 | 8篇 |
1997年 | 8篇 |
1996年 | 11篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 10篇 |
1991年 | 6篇 |
1990年 | 7篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 6篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1966年 | 2篇 |
1942年 | 2篇 |
1941年 | 3篇 |
1940年 | 3篇 |
1932年 | 2篇 |
排序方式: 共有735条查询结果,搜索用时 0 毫秒
1.
Jeroen M. G. Stevens Hilde Vervaecke Han de Vries Linda van Elsacker 《International journal of primatology》2007,28(6):1417-1430
Bonobos have a reputation as a female-dominated and egalitarian species. We examined the 2 aspects of dominance in 6 captive
bonobo groups. Females do not consistently evoke submission from all males in all contexts. Though females occupy the highest-ranking
positions in the dominance hierarchy, there are in each group males that obtain rather high ranks and are able to dominate
≥1 female. Thus female dominance is not complete and hierarchies can be better described as nonexclusive female dominance.
We studied egalitarianism by measuring linearity and steepness of dominance hierarchies. The hierarchies of all groups are
highly linear. Hierarchies among males are steeper than among females. On average, male bonobos are more despotic than females,
but females too can have despotic relations, both with other females and with males. Hence one can call bonobos in captivity
semidespotic rather than egalitarian. 相似文献
2.
Hilde Nybom 《Nordic Journal of Botany》1987,7(4):373-381
Five stands of an apomictic blackberry, Rubus nessensis , were studied. Biennial shoots flower and fruit in their second year on lateral branches developed from axillary buds. Most premature death of reproductive units occurs by withering of entire laterals, presumably due to damage by frost during the preceeding autumn or winter. Health status of the floricane, assessed as percentage live axillary buds in the spring, was positively correlated with the reproduction parameters. Shoot height as well, is positively correlated with number of flower buds and flowers produced on each lateral but, however, negatively correlated with number of berries, probably because tall shoots tend to suffer more damage by frost which seriously afflicts the vascular tissues of the laterals.
The position of the axillary buds on the shoot affects survival and degree of fertility, those situated medially greatly surpassing apical and basal ones. 相似文献
The position of the axillary buds on the shoot affects survival and degree of fertility, those situated medially greatly surpassing apical and basal ones. 相似文献
3.
This contribution illustrates the advantages of some chromophoric and fluorophoric carbohydrate derivatives such asp-nitrophenyl (pNO2Phe) or 4-methylumbelliferyl (MeUmb) glycosides andN-dansylgalactosamine in studies of the binding equilibrium and kinetics with some plant lectins. The methods used involve
continuous titrations of changes in ligand or protein absorption and ligand fluorescence, including substitution titrations
as well as stopped-flow, temperature-jump or pressure-jump relaxation kinetics.
When monitored by temperature-jump relaxation, binding of MeUmbαGal to the bloodgroup A specific lectin GSAI-A4 fromGriffonia simplicifolia is a simple bimolecular association with parametersk
+
= 9.4 × 104 M-1 s-1 andk
-1 = 5.3 s-1 at 23°C, but binding to the GSAI-B4 lectin is biphasic.
The complementarity of the peanut agglutinin binding site with Galβ1 → 3GalNAc that occurs in manyO-glycoproteins follows from enthalpic considerations and also from the value of the dissociation-rate parameterk
-1 = 0.24 s-1 of the MeUmbβGalβl → 3GalNAc.lectin complex. This value, obtained by stopped-flow kinetics is 100 times smaller than for other mono-and disaccharides
investigated. The binding mechanism is simple and the derivatisation of Galβ1 → 3GalNAc does not affect the affinity to a considerable degree.
The binding preference of tetravalentsoybean agglutinin for MeαGalNAc over MeαGal by a factor of 25 is mainly of enthalpic origin with an additional 7 kJ mol-1; the NAc group causes perturbation of a tryptophanyl residue, evidenced by protein difference absorption spectrometry. In
the glycosides, a large aglycon likeβpNO2 Phe orβMeUmb hardly affects the affinity of SBA but a largeN-dansyl group increases the affinity by a factor 20 as compared to GalNAc. The 10-fold increase in carbohydrate-specificN-dansylgalactosamine fluorescence, together with a very favourable entropic contribution point at the presence of a hydrophobic
region in the vicinity of the carbohydrate-binding site. The dissociation-rate parameter of the MeUmbβGalNAc SBA complex is slower than for any reported monosaccharide-lectin complex: 0.4 s-1.
The divalent lectin fromErythrina cristagalli preferentially binds the Galβ1 → 4GlcNAc structure that occurs in manyN-glycoproteins. The combining site was mapped thermodynamically with carbohydrates ranging from mono-to pentasaccharides as
derived fromN-glycoproteins. Here, N-dansylgalactosamine was used as a fluorescent indicator ligand in substitution titrations. When Galβ1 → 4GlcNAc was linkedα1 → 2 orα1 → 6 to Man, the binding enthalpy and entropy remained practically constant. Application of stopped flow kinetics and pressure-jump
relaxation withN-dansylgalactosamine gave mono-exponential signal changes with a concentration dependence corresponding tok
+ = 4.8 x 104 M-1 s-1
k
- = 0.4 to 0.66 s-1 and a change in reaction volume of+7ml/mol. 相似文献
4.
Characterization of varicella-zoster virus enhancement by the pesticide carbaryl. 总被引:2,自引:1,他引:1 下载免费PDF全文
Further characterization of the viral enhancement system of varicella-zoster virus by the pesticide carbaryl (1-naphthyl-N-methylcarbamate) is presented. It was necessary to expose cells to the enhancing chemical during the period of virus replication to detect enhancement. The optimum time for the pretreatment is 20 to 24 h. Maximum enhancement of virus expression occurs 48 to 72 h post-inoculation. Treated cells cannot pass on to daughter cells the ability to produce increased amounts of virus. alpha-Naphthol, a metabolite of carbaryl, is also capable of enhancing virus replication; Guthion, another pesticide tested, did not enhance varicella-zoster virus. The stable cell line HEP-2 can be used in place of human embryonic lung cells to detect enhancement. Differences in enhancement levels were not due to cell lot, cell passage, or a change in the stability of the cell membrane to sonic disruption. 相似文献
5.
Hilde Beele Hubert Thierens Leo de Ridder 《In vitro cellular & developmental biology. Plant》1989,25(10):923-933
Summary Different organotypical culture methods are used to test the direct effects of serotonin and ketanserin, a S2, α1, and H1 receptor antagonist in vascular tissue, on fibroblasts and epidermal cells of embryonic chick skin in vitro. From light microscopic
and electron microscopic analyses, we learn that serotonin enhances keratinization and differentiation, whereas ketanserin
reduces differentiation in comparison to the control cultures. Incorporation data of fragments cultured with [3H]thymidine show that ketanserin, within a dose range from 0.05 to 5 μg/ml, stimulates proliferation. Serotonin at a concentration
of 10 μg/ml slightly slows down proliferation, whereas lower doses of 0.1 and 1 μg/ml result in tritium activities that do
not differ from control cultures.
This investigation was financially supported by the National Fund of Scientific Research, Belgium, 3.0022.87. 相似文献
6.
7.
Gerardo H. Vázquez Nin Ernestina Flores Olga M. Echeverría Hilde Merkert Rodolfo Wettstein Ricardo Benavente 《Chromosoma》1993,102(7):457-463
The distribution of DNA in synaptonemal complexes of rat and mouse spermatocytes, and of chick oocytes was investigated by immunogold electron microscopy. Except for a few specific sites, DNA was not immunolocalized in the space between lateral elements of the complex. Some labeled fibrils connecting the lateral elements with the central element were observed associated with recombination nodules or near them. However, other labeled fibrils in the space between lateral elements did not appear to present any relationship to recombination nodules. The immunocytochemical approaches used here confirmed the presence of significant amounts of DNA in the lateral elements as previously indicated by preferential DNA staining methods. Furthermore, our findings support the view that recombination nodules are the site of chiasma formation. 相似文献
8.
Bjørn-Yngvar Nordvåg Ida Ranløv Hilde Monica Frostad Riise Gunnar Husby M. Raafat El-Gewely 《Human genetics》1993,92(3):265-268
Severe familial amyloid cardiomyopathy (FAC) in a Danish kindred is associated with a specific mutation (Met for Leu 111) in the transthyretin (TTR) gene. The mutation causes the loss of a DdeI restriction site in the gene, allowing molecular diagnostic studies. We studied formalin-fixed, paraffin-embedded tissues, up to 39 years old, from 29 family members of this kindred. DNA was partially purified from deparaffinized tissue sections and a DNA sequence of the TTR gene flanking the mutation site was amplified by the polymerase chain reaction (PCR), followed by restriction enzyme analysis. Amplified DNA was obtained from tissues representing 23 of the 29 persons. Ten out of the 23 family members were found to carry the TTR Met 111 mutation, whereas 13 were not affected. The results were consistent with known clinical data and with corresponding serum TTR examinations. This retrospective study shows that archival tissues can be used to confirm the diagnosis and disease pattern in members of families affected by hereditary diseases. 相似文献
9.
Hilde Nybom 《Folia Geobotanica》1996,31(3):295-304
DNA fingerprinting has proved useful for the analysis of genotypic distribution and estimation of genetic relatedness in apomictic plant groups likeRubus, Amelanchier, Hieracium andTaraxacum. Speciation through interspectific hybridization has been demonstrated in one case involving apomicticRubus species. The application of molecular markers to progeny derived from experimental cross-pollinations has yielded information on levels of apomixis as well as on its inheritance and regulation in e.g.Rubus, Poa, Pennisetum andTripsacum. 相似文献
10.
K Abrahamsen H L Kong A Mastrangeli D Brough A Lizonova R G Crystal E Falck-Pedersen 《Journal of virology》1997,71(11):8946-8951
A strategy for constructing replication-defective adenovirus vectors from non-subgroup C viruses has been successfully demonstrated with adenovirus type 7 strain a (Ad7a) as the prototype. An E1A-deleted Ad7a reporter virus expressing the chloramphenicol acetyltransferase (CAT) gene from the cytomegalovirus promoter enhancer was constructed with DNA fragments isolated from Ad7a, an Ad7a recombination reporter plasmid, and the 293 cell line. The Ad7a-CAT virus particle transduces A549 cells as efficiently as Ad5-based vectors. Intravenous infections in a murine model indicate that the Ad7a-CAT virus infects a variety of tissues, with maximal levels of CAT gene expression found in the liver. The duration of Ad7a-CAT transgene expression in the liver was maximally maintained 2 weeks postinfection, with a decline to baseline activity by the week 4 postinfection. Ad7a-CAT represents the first example of a non-subgroup C E1A- adenovirus gene transfer vector. 相似文献