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New observations on the bioluminescence of echinoderms 总被引:3,自引:0,他引:3
Bioluminescence has been observed in two species of Ophiuroidea, six species of Asteroidea, nine species of Holothuroidea and two species of Crinoidea. The luminescent responses are described and the intensities and time courses of the responses of some species are given.
Histological investigations have indicated possible luminous cells in the Holothuroidea, but the results in other classes remain equivocal. Preliminary analyses of the chemistry of bioluminescence indicate that a similar system may be responsible for bioluminescence in all four classes, although the visible manifestations of the system vary considerably. 相似文献
Histological investigations have indicated possible luminous cells in the Holothuroidea, but the results in other classes remain equivocal. Preliminary analyses of the chemistry of bioluminescence indicate that a similar system may be responsible for bioluminescence in all four classes, although the visible manifestations of the system vary considerably. 相似文献
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The periodate oxidation of bovine bone sialoprotein, and some observations on its structure 总被引:2,自引:1,他引:1
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1. Bovine bone sialoprotein (mol.wt. 23000) contains N-acetylneuraminic acid and N-glycollylneuraminic acid, fucose, galactose, mannose, N-acetylgalactosamine and N-acetylglucosamine residues in the form of a very small number, perhaps one, of highly branched oligosaccharide structures linked covalently to peptide. 2. Periodate oxidation of the sialoprotein results in quantitative destruction only of the sialic acid and fucose residue consistent with the earlier findings of their positions as terminal groups. 3. Terminal sialic acid residues are attached to galactopyranose residues by 2,3-linkages, and to some N-acetylgalactosamine residues (at C-6). 4. Sequential Smith degradation indicates that N-acetylgalactosamine residues may be present as points of branching (linked in C-1, C-3 and C-6) and N-acetylglucosamine residues are located in the inner part of the structure, adjacent to the carbohydrate–peptide bond(s). 5. Mannose residues appear to be linked in the 1,3-positions. 相似文献
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Micronektonic decapod crustaceans were sampled repeatedly ata depth of 200 ± 25 m at two positions centred on 20°30'N,19°40'W and 31°10'N, 21°05'W to assess their responseto an artificial light fitted to an opening/closing rectangularmidwater trawl. Of the 20 decapod species identified, the numbersand biomass of the three numerically dominant diel migrantsOplophorus spinostu, Sysiellaspis debilis and Acanthephyra purpureawere significantly reduced in hauls taken with the artificiallight switched on, when compared with catches with it switchedoff. There was little measurable response in the frequentlyoccurring species Gennadas valens or Gennadas brevirostris,or in other less frequently occurring species. 相似文献
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Irving H. Fox Thomas D. Palella David Thompson Charles Herring 《Archives of biochemistry and biophysics》1982,215(1):302-308
To elucidate potential toxic properties of S-adenosylhomocysteine and 5′-methylthioadenosine, we have examined the inhibitory properties of these compounds upon enzymes involved with adenosine metabolism. S-Adenosylhomocysteine, but not S-adenosylmethionine, was a noncompetitive inhibitor of adenosine kinase with Ki values ranging from 100 to 400 μm. Methylthioadenosine competitively inhibited adenosine kinase with variable adenosine below 1 μm with a Ki of 120 μm, increased adenosine kinase activity when the adenosine concentration exceeded 2 μm, and did not appear to be a substrate for adenosine kinase. Methylthioadenosine inactivated S-adenosylhomocysteine hydrolase from erythrocytes, B-lymphoblasts, and T-lymphoblasts with Ki values ranging from 65 to 117 μm and “k2” from 0.30 to 0.55 min?1. Adenosine deaminase was not inhibited by 5′-methylthioadenosine up to 1000 μm. To clarify how 5′-methylthioadenosine might accumulate, 5′-methylthioadenosine phosphorylase was evaluated. This enzyme was not blocked by up to 500 μm adenosine, deoxyadenosine, S-adenosylhomocysteine, or S-adenosylmethionine and was not decreased in erythrocytes from patients with adenosine deaminase deficiency, purine nucleoside phosphorylase deficiency, or hypogammaglobulinemia. These observations suggest that the inhibitory properties of 5′-methylthioadenosine upon adenosine kinase and S-adenosylhomocysteine hydrolase may contribute to the toxicity of the exogenously added compound. The toxicity resulting from S-adenosylhomocysteine accumulation intracellularly may be related to adenosine kinase inhibition in addition to disruption of transmethylation reactions. 相似文献
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K S Lau W J Herring J L Chuang M McKean D J Danner R P Cox D T Chuang 《The Journal of biological chemistry》1992,267(33):24090-24096
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S A Leachman P J Gallagher B P Herring M J McPhaul J T Stull 《The Journal of biological chemistry》1992,267(7):4930-4938
The molecular and biochemical properties of myosin light chain kinases from chicken skeletal and smooth muscle were investigated by recombinant DNA techniques. Deletion of the amino-terminal region of either the smooth or skeletal muscle myosin light chain kinase resulted in a decrease in Vmax with no significant change in Km values for light chain substrates. Skeletal/smooth muscle chimeric kinases were inactive when a 65-residue region amino-terminal of the catalytic core was exchanged between the two forms. Changing alanine 494 to glutamic acid within this region in the chicken skeletal muscle myosin light chain kinase increased the Km values for light chains 10-fold. These results are consistent with the hypothesis that the region amino-terminal of the catalytic core in myosin light chain kinases is involved in light chain recognition. A skeletal muscle kinase which contained the smooth muscle calmodulin binding domain remained regulated by Ca2+/calmodulin. Thus, the calmodulin binding domains of smooth and skeletal muscle myosin light chain kinases share structural elements necessary for regulation. 相似文献
10.
D P Fitzsimons B P Herring J T Stull P J Gallagher 《The Journal of biological chemistry》1992,267(33):23903-23909
It is postulated that basic residues in the regulatory region of myosin light chain kinase are important for conferring autoinhibition by binding to the catalytic core. To investigate this proposal, 10 basic amino acids within the regulatory region of rabbit smooth muscle myosin light chain kinase (Lys961-Lys979) were replaced either singularly or in combination with acidic or nonpolar residues by site-directed mutagenesis. All active mutant kinases were dependent on Ca2+/calmodulin for catalytic activity. None of the mutants was active in the absence of Ca2+/calmodulin, suggesting that the autoinhibitory region has not been defined completely. Charge reversal mutants at Arg974, Arg975, and Lys976 resulted in loss of high affinity binding of calmodulin and increased the concentration of calmodulin required for half-maximal activation (KCaM). The charge reversal mutant at Lys979 also increased KCaM but to a lesser extent. Charge reversal mutants at Lys965 and Arg967 resulted in an inactive myosin light chain kinase that could not be proteolytically activated. When these residues were mutated to Ala, the expressed kinase was dependent upon Ca2+/calmodulin for activity and exhibited a decrease in KCaM. Charge reversal mutants in Lys961 and Lys962 also had decreased KCaM values. These basic residues amino-terminal of the calmodulin binding domain may play an important role in the activation of the kinase. 相似文献