全文获取类型
收费全文 | 3751篇 |
免费 | 228篇 |
国内免费 | 2篇 |
出版年
2021年 | 32篇 |
2017年 | 29篇 |
2016年 | 61篇 |
2015年 | 91篇 |
2014年 | 104篇 |
2013年 | 159篇 |
2012年 | 146篇 |
2011年 | 152篇 |
2010年 | 134篇 |
2009年 | 128篇 |
2008年 | 166篇 |
2007年 | 184篇 |
2006年 | 140篇 |
2005年 | 131篇 |
2004年 | 133篇 |
2003年 | 108篇 |
2002年 | 140篇 |
2001年 | 60篇 |
2000年 | 52篇 |
1999年 | 51篇 |
1998年 | 48篇 |
1997年 | 54篇 |
1996年 | 44篇 |
1995年 | 47篇 |
1994年 | 39篇 |
1993年 | 39篇 |
1992年 | 39篇 |
1991年 | 41篇 |
1990年 | 40篇 |
1989年 | 46篇 |
1988年 | 36篇 |
1987年 | 39篇 |
1986年 | 26篇 |
1985年 | 35篇 |
1984年 | 42篇 |
1982年 | 40篇 |
1981年 | 37篇 |
1980年 | 28篇 |
1979年 | 29篇 |
1977年 | 28篇 |
1976年 | 32篇 |
1973年 | 32篇 |
1965年 | 46篇 |
1964年 | 26篇 |
1963年 | 47篇 |
1962年 | 32篇 |
1961年 | 40篇 |
1960年 | 38篇 |
1959年 | 35篇 |
1958年 | 33篇 |
排序方式: 共有3981条查询结果,搜索用时 93 毫秒
1.
Robert Blake II Elizabeth A. Shute James Waskovsky Arthur P. Harrison JR. 《Geomicrobiology journal》2013,30(3-4):173-192
Abstract Microorganisms capable of aerobic respiration on ferrous ions are spread throughout eubacterial and archaebacterial phyla. Phylogenetically distinct organisms were shown to express spectrally distinct redox‐active biomolecules during autotrophic growth on soluble iron. A new iron‐oxidizing eubacterium, designated as strain Funis, was investigated. Strain Funis was judged to be different from other known iron‐oxidizing bacteria on the bases of comparative lipid analyses, 16S rRNA sequence analyses, and cytochrome composition studies. When grown autotrophically on ferrous ions, Funis produced conspicuous levels of a novel acid‐stable, acid‐soluble yellow cytochrome with a distinctive absorbance peak at 579 nm in the reduced state. Stopped‐flow spectrophotometric kinetic studies were conducted on respiratory chain components isolated from cell‐free extracts of Thiobacillus ferrooxidans. Experimental results were consistent with a model where the primary oxidant of ferrous ions is a highly aggregated c‐type cytochrome that then reduces the periplasmic rusticyanin. The Fe(II)‐dependent, cytochrome c‐catalyzed reduction of the rusticyanin possessed three kinetic properties in common with corresponding intact cells that respire on iron: the same anion specificity, a similar dependence of the rate on the concentration of ferrous ions, and similar rates at saturating concentrations of ferrous ions 相似文献
2.
3.
Hermann Desselberger 《Journal of Ornithology》1930,78(1):86-106
Ohne Zusammenfassung 相似文献
4.
5.
6.
Michaela Ludolphs Daniela Schneeberger Tolga Soykan Jonas Sch?fer Theofilos Papadopoulos Nils Brose Hermann Schindelin Claudia Steinem 《The Journal of biological chemistry》2016,291(1):244-254
The regulatory protein collybistin (CB) recruits the receptor-scaffolding protein gephyrin to mammalian inhibitory glycinergic and GABAergic postsynaptic membranes in nerve cells. CB is tethered to the membrane via phosphoinositides. We developed an in vitro assay based on solid-supported 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine membranes doped with different phosphoinositides on silicon/silicon dioxide substrates to quantify the binding of various CB2 constructs using reflectometric interference spectroscopy. Based on adsorption isotherms, we obtained dissociation constants and binding capacities of the membranes. Our results show that full-length CB2 harboring the N-terminal Src homology 3 (SH3) domain (CB2SH3+) adopts a closed and autoinhibited conformation that largely prevents membrane binding. This autoinhibition is relieved upon introduction of the W24A/E262A mutation, which conformationally “opens” CB2SH3+ and allows the pleckstrin homology domain to properly bind lipids depending on the phosphoinositide species with a preference for phosphatidylinositol 3-monophosphate and phosphatidylinositol 4-monophosphate. This type of membrane tethering under the control of the release of the SH3 domain of CB is essential for regulating gephyrin clustering. 相似文献
7.
8.
9.
Hoffmann Hermann 《Molecular & general genetics : MGG》1922,29(1):144
Ohne Zusammenfassung 相似文献
10.
KHALIFAH R. A.; LEWIS L. N.; COGGINS C. W. JR.; RADLICK P. C. 《Journal of experimental botany》1965,16(3):511-517
Evidence for the non-indolic nature of the new citrus auxinis presented on the basis of fluorometric properties, thin-layerchromatography, Ehrlich's colour reaction, paper electrophoresis,and the infra-red spectra determinations. Citrus auxin had alower Rf in TLC than IAA, did not give the typical indole reactionwith Ehrlich's reagent, and behaved differently in electrophoresis.The infra-red spectra also provided preliminary informationconcerning chemical structure. The hypothesis that indolic compoundsconstitute the only natural auxins in higher plants should berevised in view of this evidence that a non-indole auxin existsin higher plants. 相似文献