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The chemiluminescent reaction of luminol during lipoxygenase-catalyzed oxygenations was studied with the purpose of developing a specific luminometric assay for cis,cis-1,4-pentadiene fatty acids directly in aqueous solutions. The addition of picomole levels of either linoleic or arachidonic acids to reaction systems containing 0.04 mM luminol and 40 micrograms/ml of purified soybean lipoxygenase-1 gave light emission curves with a single sharp maximum. Under these conditions the peak heights were linearly dependent on the fatty acid concentration and the detection limit for both of the fatty acids was 2 pmol with a signal to noise ratio of 2. For maximum reproducibility of the assays a procedure for the proper quantitation of the enzyme was developed. The fact that the assay proved to be relatively interference-free was ascribed to the high molar enzyme/substrate ratio (above 1). 相似文献
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Sanna Loppi Paula Korhonen Maria Bouvy‐Liivrand Simone Caligola Tiia A. Turunen Mikko P. Turunen Ana Hernandez de Sande Natalia Koosowska Flavia Scoyni Anna Rosell Teresa García‐Berrocoso Sighild Lemarchant Hiramani Dhungana Joan Montaner Jari Koistinaho Katja M. Kanninen Minna U. Kaikkonen Rosalba Giugno Merja Heinniemi Tarja Malm 《Aging cell》2021,20(1)
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Allison Jones Andrew E. Teschendorff Quanxi Li Jane D. Hayward Athilakshmi Kannan Tim Mould James West Michal Zikan David Cibula Heidi Fiegl Shih-Han Lee Elisabeth Wik Richard Hadwin Rupali Arora Charlotte Lemech Henna Turunen P?ivi Pakarinen Ian J. Jacobs Helga B. Salvesen Milan K. Bagchi Indrani C. Bagchi Martin Widschwendter 《PLoS medicine》2013,10(11)
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Hydrobiologia - Forestry-related land use can cause increasing instream sedimentation, burying and eradicating stream bryophytes, with severe ecological consequences. However, there is limited... 相似文献
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Laukkanen MO Lehtolainen P Turunen P Aittomäki S Oikari P Marklund SL Ylä-Herttuala S 《Gene》2000,254(1-2):173-179
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Kari Taulavuori Henna Pihlajaniemi Satu Huttunen Erja Taulavuori 《Trees - Structure and Function》2011,25(2):231-236
Several deciduous woody plant species produce anthocyanins during leaf development in spring and again during leaf senescence
in autumn. The leaves of Betula pendula Roth (silver birch) commonly exhibit transient reddening in juvenile leaves under northern growing conditions, with the intensity
of the red colour varying among individual trees. The objective of our study was to test the hypothesis that the accumulation
of foliar anthocyanins during spring in leaves of B. pendula is an ecotypic response. Chlorophyll fluorescence ratio (Fv/Fm), leaf reflectance and anthocyanin concentrations were measured,
in relation to phenology in spring, summer and autumn from birches used for landscaping with either red or green-emergent
leaves. The results suggest that (1) the trees with green- or red-emergent juvenile leaves represent different populations,
and (2) that the red-emergent leaves senesced earlier, indicating that (3) trees with red-emerging leaves belong to a more
northern ecotype than the trees with green-emerging leaves. The role of anthocyanin synthesis in a northern radiation environment
is discussed. 相似文献