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对分离自南京土壤中的一株高产水溶性蓝色素的放线菌菌株Streptomyces sp.进行了细胞化学组分及16S rDNA序列分析。细胞化学组分分析结果表明待定菌株的细胞壁含LL-DAP及甘氨酸,全细胞水解物含葡萄糖及核糖,全细胞脂肪酸组分主要为14-17碳的脂肪酸,其中anteiso-15和iso-16为主要组分,应归入链霉菌属。基于16S rDNA序列的聚类结果表明所选菌株基本分成9个分支,能够产生可溶性蓝色素的菌株聚成其中两个分支,即以S.coelicolor为代表的分支和以S.cyaneus为代表的分支,待定菌株与Streptomyces in-digocolor的相似性为99.4%,属于S.cyaneus分支。 相似文献
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Tao Cong-Qi Ding Yi Zhao Yang-Jie Cui Heng-Lin 《Journal of microbiology (Seoul, Korea)》2020,58(2):105-112
Journal of Microbiology - Two halophilic archaeal strains, SHR37T and NEN6, were isolated from salt lakes located in the Tibet and Xinjiang regions of China. The two strains were found to form a... 相似文献
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Yun-Zhuang Mou Xing-Xing Qiu Mei-Lin Zhao Heng-Lin Cui Dickson Oh Mike L. Dyall-Smith 《Extremophiles : life under extreme conditions》2012,16(6):895-901
Two halophilic archaeal strains, R30T and tADLT, were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120?g/l NaCl and 48–67?g/l MgCl2 for growth but differ in their optimum growth temperatures (30?°C, tADLT vs. 40?°C, R30T). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4?% identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB′ gene similarity between strains R30T and tADLT is 92.9?% and less to other halobacteria. Their DNA G?+?C contents are 62.4–62.9?mol?% but DNA–DNA hybridization gives a relatedness of only 44?%. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30T (=?CGMCC 1.10593T?=?JCM 17270T) and strain tADLT (=?JCM 15066T?=?DSMZ 22187T) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively. 相似文献
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Two halophilic archaea, strains GX21T and R35T, were isolated from a marine solar saltern and an aquaculture farm in China, respectively. Cells of the two strains were observed to be pleomorphic, flat, to contain gas vesicles, stain Gram-negative and produce red-pigmented colonies. Strain GX21T was found to be able to grow at 25–50 °C (optimum 37 °C), at 2.6–4.8 M NaCl (optimum 3.4 M NaCl), at 0.05–1.0 M MgCl2 (optimum 0.1 M MgCl2) and at pH 6.0–8.5 (optimum pH 6.5) while strain R35T was found to be able to grow at 25–45 °C (optimum 37 °C), at 2.1–4.8 M NaCl (optimum 3.1 M NaCl), at 0–0.7 M MgCl2 (optimum 0.03 M MgCl2) and at pH 5.5–9.5 (optimum pH 6.5–7.0). The cells of both isolates were observed to lyse in distilled water. The minimum NaCl concentrations that prevented cell lysis were determined to be 15 % (w/v) for strain GX21T and 12 % (w/v) for strain R35T. The major polar lipids of the two strains were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, one major glycolipid and a minor lipid chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. 16S rRNA gene sequence analysis revealed that strains GX21T and R35T show 97.1 % sequence similarity to each other and are closely related to Haloplanus aerogenes TBN37T (96.8 and 95.8 %), Haloplanus vescus RO5-8T (96.7 and 96.1 %), Haloplanus salinus YGH66T (96.4 and 95.8 %) and Haloplanus natans JCM 14081T (96.3 and 95.4 %). The rpoB′ gene similarity between strains GX21T and R35T is 90.5 % and show 88.5–90.8 % similarity to the Haloplanus species with validly published names. The DNA G+C content of strain GX21T and R35T were determined to be 65.8 and 66.0 mol%, respectively. The DNA–DNA hybridization values between strain GX21T and strain R35T, and the two strains with the Haloplanus species with validly published names, showed less than 50 % DNA–DNA relatedness. It was concluded that strain GX21T (=CGMCC 1.10456T = JCM 17092T) and strain R35T (=CGMCC 1.10594 T = JCM 17271T) represent two new species of Haloplanus, for which the names Haloplanus litoreus sp. nov. and Haloplanus ruber sp. nov. are proposed. 相似文献