全文获取类型
收费全文 | 1100篇 |
免费 | 73篇 |
出版年
2022年 | 8篇 |
2021年 | 13篇 |
2020年 | 6篇 |
2019年 | 12篇 |
2018年 | 12篇 |
2017年 | 17篇 |
2016年 | 18篇 |
2015年 | 46篇 |
2014年 | 53篇 |
2013年 | 56篇 |
2012年 | 71篇 |
2011年 | 68篇 |
2010年 | 48篇 |
2009年 | 56篇 |
2008年 | 62篇 |
2007年 | 48篇 |
2006年 | 45篇 |
2005年 | 38篇 |
2004年 | 38篇 |
2003年 | 47篇 |
2002年 | 48篇 |
2001年 | 23篇 |
2000年 | 16篇 |
1999年 | 28篇 |
1998年 | 20篇 |
1997年 | 9篇 |
1996年 | 13篇 |
1995年 | 17篇 |
1994年 | 17篇 |
1993年 | 12篇 |
1992年 | 19篇 |
1991年 | 15篇 |
1990年 | 8篇 |
1989年 | 11篇 |
1988年 | 10篇 |
1987年 | 8篇 |
1986年 | 7篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1978年 | 10篇 |
1974年 | 6篇 |
1973年 | 6篇 |
1972年 | 8篇 |
1971年 | 6篇 |
1967年 | 6篇 |
1966年 | 5篇 |
排序方式: 共有1173条查询结果,搜索用时 15 毫秒
1.
2.
Thea Kristiansen Jens‐Christian Svenning Wolf L. Eiserhardt Dennis Pedersen Hans Brix Søren Munch Kristiansen Maria Knadel César Grández Henrik Balslev 《Journal of Biogeography》2012,39(7):1318-1332
Aim It is a central issue in ecology and biogeography to understand what governs community assembly and the maintenance of biodiversity in tropical rain forest ecosystems. A key question is the relative importance of environmental species sorting (niche assembly) and dispersal limitation (dispersal assembly), which we investigate using a large dataset from diverse palm communities. Location Lowland rain forest, western Amazon River Basin, Peru. Methods We inventoried palm communities, registering all palm individuals and recording environmental conditions in 149 transects of 5 m × 500 m. We used ordination, Mantel tests and indicator species analysis (ISA) to assess compositional patterns, species responses to geographical location and environmental factors. Mantel tests were used to assess the relative importance of geographical distance (as a proxy for dispersal limitation) and environmental differences as possible drivers of dissimilarity in palm species composition. We repeated the Mantel tests for subsets of species that differ in traits of likely importance for habitat specialization and dispersal (height and range size). Results We found a strong relationship between compositional dissimilarity and environmental distance and a weaker but also significant relationship between compositional dissimilarity and geographical distance. Consistent with expectations, relationships with environmental and geographical distance were stronger for understorey species than for canopy species. Geographical distance had a higher correlation with compositional dissimilarity for small‐ranged species compared with large‐ranged species, whereas the opposite was true for environmental distance. The main environmental correlates were inundation and soil nutrient levels. Main conclusions The assembly of palm communities in the western Amazon appears to be driven primarily by species sorting according to hydrology and soil, but with dispersal limitation also playing an important role. The importance of environmental characteristics and geographical distance varies depending on plant height and geographical range size in agreement with functional predictions, increasing our confidence in the inferred assembly mechanisms. 相似文献
3.
Kirsten Skamstrup Hansen Stefan Vieths Helle Vestergaard Per Stahl Skov Carsten Bindslev-Jensen Lars K. Poulsen 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2001,756(1-2)
The aim of the study was to investigate the possibility of a seasonal variation in reactivity to apples in 27 birch pollen allergic patients. Before and during the birch pollen season 1998, the patients were subjected to double-blind, placebo-controlled food challenges (DBPCFCs) with grated fresh Golden Delicious apple followed by an open food challenge with whole fresh apple. The clinical reactions elicited during the challenges were evaluated both by the patients and the investigators. Moreover, the skin reactivity and the in vitro reactivity to apple were evaluated by skin prick test (SPT), leukocyte histamine release (HR), measurement of specific IgE, and immunoblotting experiments. The sensitivity of the DBPCFC, when compared with the result of the open challenge, was 0.74 (14/19) before the season and 0.80 (16/20) during the season. None of the patients reacted to the blinded challenge without a subsequent reaction to the open challenge. One placebo reaction was registered both before and in season, but not in the same patient. The patient scores of the first positive challenges, and the maximal scores of each combined blinded and open challenge session, were significantly increased during the pollen season (P<0.05). The scores of the open challenge were significantly higher than the scores of the DBPCFC both before the season and during the in-season challenges (P<0.05). Specific IgE against Golden Delicious increased during season (P<0.05), while neither SPT, HR, nor immunoblotting experiments could confirm an increase in reactivity. In conclusion, the results of the oral challenge tests indicated an increase in clinical reactivity to apples during the birch pollen season in birch pollen allergic individuals. 相似文献
4.
5.
The effects of growing Clostridium acetobutylicum NCIB 8052 in a chemostat under conditions of glucose, NH(4) (+), PO(4) (3-), Mg(2+), and Fe(2+) limitation were examined. It was noted that limitation of any major nutrient resulted in the same fermentation pattern. Conditions where minor nutrient levels were reduced appeared to stimulate solvent production. Under conditions of Mg(2+) restriction, a productive solventogenic culture was produced, with a yield of total solvents on glucose of 35.5%. 相似文献
6.
Unusual ciliate-specific codons in Tetrahymena mRNAs are translated correctly in a rabbit reticulocyte lysate supplemented with a subcellular fraction from Tetrahymena. 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The codon usage of Tetrahymena thermophila and other ciliates deviates from the 'universal genetic code' in that UAA and probably UAG are not translational termination signals but code for glutamine. Therefore, translation in vitro of mRNA from Tetrahymena in a reticulocyte lysate is prematurely terminated if a UAA or UAG triplet is present in the reading frame of the mRNA. We show that the addition of a subcellular fraction from Tetrahymena thermophila enables a rabbit reticulocyte lysate to translate Tetrahymena mRNAs into full-sized proteins. The activity of the subcellular fraction is shown to depend on the combined function of a protein component(s) and a tRNA(s). The subcellular fraction is easily prepared and its usefulness for the identification of isolated mRNAs from Tetrahymena by their translation products in vitro is demonstrated. 相似文献
7.
8.
9.
We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing. 相似文献