全文获取类型
收费全文 | 531篇 |
免费 | 48篇 |
国内免费 | 4篇 |
出版年
2022年 | 6篇 |
2021年 | 9篇 |
2019年 | 3篇 |
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 13篇 |
2015年 | 23篇 |
2014年 | 25篇 |
2013年 | 22篇 |
2012年 | 43篇 |
2011年 | 46篇 |
2010年 | 29篇 |
2009年 | 21篇 |
2008年 | 20篇 |
2007年 | 25篇 |
2006年 | 19篇 |
2005年 | 13篇 |
2004年 | 18篇 |
2003年 | 20篇 |
2002年 | 12篇 |
2001年 | 13篇 |
2000年 | 17篇 |
1999年 | 7篇 |
1998年 | 10篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1992年 | 6篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1986年 | 4篇 |
1985年 | 8篇 |
1984年 | 4篇 |
1983年 | 13篇 |
1981年 | 4篇 |
1979年 | 7篇 |
1978年 | 9篇 |
1977年 | 4篇 |
1975年 | 4篇 |
1974年 | 8篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1970年 | 5篇 |
1969年 | 6篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1966年 | 2篇 |
排序方式: 共有583条查询结果,搜索用时 15 毫秒
1.
2.
3.
Scott T. Sayers Hock C. Yeoh Jerry A. McLane Irene R. Held 《Neurochemical research》1988,13(12):1125-1131
The acetylcholine receptor (AChR) content and the autorphosphorylation of the regulatory subunit of cyclic AMP-dependent protein kinase type II (R-II) were evaluated in rat soleus muscles at 24, 30 and 66 hr after surgical denervation by cutting the nerve at a short distance (short-nerve-stump) and at a long distance (long-nerve-stump) from the muscle. AChR content was based on the specific binding of [125I]alpha-bungarotoxin (BUTX); changes in the autophosphorylation of R-II were based upon the predominant in vitro32P-phosphorylation of a 56-Kd soluble protein in cytosolic fractions of solei. The AChR content and the32P-autophosphorylation of R-II were increased in samples from short-nerve-stump solei, but not from long-nerve-stump solei, after a denervation-time of 30 hr. This nerve-stump-length dependency indicates that the two denervation effects are not related to the immediate halt of impulse-evoked muscle contractility. Furthermore, the results show that alterations in the32P-autophosphorylation of R-II occurred before, as well as whenever, increases in the AChR content were found. Speculatively, this temporal relationship may be significant with respect to the potential role of R-II in gene expression.Abbreviations ACh
acetylcholine
- AChR
acetylcholine receptor(s)
- BUTX
alpha-bungarotoxin
- Kd
kilodalton
- PAGE
polyacrylamide gel electrophoresis
- R-II
regulatory subunit of cyclic AMP-dependent protein kinase type II
- SDS
sodium dodecyl sulfate 相似文献
4.
Maria L. Boccia Martin L. Reite Kristine Kaemingk Polly Held Mark L. Laudenslager 《Primates; journal of primatology》1991,32(2):255-263
The social environment affects both behavioral and physiological responses to separation from the mother. Less information
is available on the impact of the social environment on the response to separation in peer-reared infant monkeys. This study
reports the responses of peer-reared pigtail macaque infants to repeated separations, and the impact of social versus isolation
housing during the separation. The responses of two pairs of monkeys were studied during four three-day separations. One of
each pair was housed in isolation during the separation, and the other was with another pair of peers, with whom they had
been living for one month prior to the separation. The isolation-housed peer responded to the separation with behavioral agitation,
but no depression. The socially-housed peer's behavior did not differ from baseline during the separation. During successive
reunions, all the separated monkeys, regardless of housing condition, exhibited declining levels of behaviors related to maintaining
proximity to their attachment figure. Although the number of subjects is small, the results suggest that the presence of social
support, in the form of a familiar peer, can ameliorate the response to separation, and that with repeated separations the
responses of the monkeys changes significantly. 相似文献
5.
Only part of the protoxin gene of Bacillus thuringiensis subsp. berliner 1715 is necessary for insecticidal activity. 总被引:1,自引:0,他引:1 下载免费PDF全文
Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicating that only a part of the protoxin gene is required for insecticidal activity. 相似文献
6.
K D Held H A Harrop B D Michael 《International journal of radiation biology and related studies in physics, chemistry, and medicine》1984,45(6):627-636
The absolute rate for the repair reaction of radiation-induced, oxygen-dependent lesions in bacterial transforming DNA with the sulphydryl (SH)-containing compound dithiothreitol (DTT) has been determined using a fast response method, the gas explosion technique, to be 1.6 X 10(6) mol-1 s-1. Glutathione reacts ten times slower than DTT with the irradiated transforming DNA. It can also be calculated that transforming DNA radicals react with O2 in a damage-fixing reaction with a rate of about 3 X 10(8) dm3 mol-1 s-1. These rates are compared with values in the literature for reaction rates of SH, compounds and O2 with irradiated DNA constituents and with bacterial cells. 相似文献
7.
8.
Dr. Abraham A. Held 《Archives of microbiology》1974,95(1):97-114
Summary This study examined the behavior of populations of zoospores of the obligately parasitic, endobiotic chytridRozella allomycis towards young, vegetative thalli of various saprophytic fungi, in order to identify host-dependent factors which control
the development ofRozella. An inverted microscope was employed for continuous observation of parasite-host interaction in petri dishes of broth or
agar medium. Two factors appear to control the initial stages of invasion byRozella of both susceptible and resistant species of the host genus,Allomyces: (i) a soluble exudate which attractsRozella zoospores, (ii) a receptor on the cell-wall surface which causesRozella zoospores to adhere, and to encyst and to germinate immediately thereafter. A related, nonsusceptible species,Blastocladiella emersonii, also attractsRozella zoospores, but supports very limited attachment.Rozella zoospores neither accumulate around, nor adhere to young thalli of non-blastocladialean fungi. This host-specific behavior
pattern is compared with that of saprophytic and facultatively parasitic Phycomycetes, whose zoospores show nonspecific chemotactic
responses and require no receptor for attachment, encystment and germination. 相似文献
9.
Nielsen J; Peixoto AA; Piccin A; Costa R; Kyriacou CP; Chalmers D 《Molecular biology and evolution》1994,11(6):839-853
The region of the clock gene period (per) that encodes a repetitive tract
of threonine-glycine (Thr-Gly) pairs has been compared between Dipteran
species both within and outside the Drosophilidae. All the non-
Drosophilidae sequences in this region are short and present a remarkably
stable picture compared to the Drosophilidae, in which the region is much
larger and extremely variable, both in size and composition. The
accelerated evolution in the repetitive region of the Drosophilidae appears
to be mainly due to an expansion of two ancestral repeats, one encoding a
Thr-Gly dipeptide and the other a pentapeptide rich in serine, glycine, and
asparagine or threonine. In some drosophilids the expansion involves a
duplication of the pentapeptide sequence, but in Drosophila pseudoobscura
both the dipeptide and the pentapeptide repeats are present in larger
numbers. In the nondrosophilids, however, the pentapeptide sequence is
represented by one copy and the dipeptide by two copies. These observations
fulfill some of the predictions of recent theoretical models that have
simulated the evolution of repetitive sequences.
相似文献
10.
A. Schreiber G. Ntzold Manuela Held 《Journal of Zoological Systematics and Evolutionary Research》1993,31(1):64-79
As a contribution to their taxonomy, population genetic data on zoo-living anoas are reported, and a review of the history of the captive stock is provided. Four different chromosome numbers of 44, 45, 47 and 48 chromosomes have been found, respectively, when karyotyping captive anoas descending from three breeding lines. The number of chromosome arms is 60 throughout, indicating that Robertsonian rearrangements are responsible for this cytogenetic variation. An electrophoretic comparison of isozymes and blood proteins representing 21 genetic loci revealed polymorphism in seven loci: haemoglobin, glyoxalase, superoxide dismutase, phosphoglucomutase, carbonic anhydrase, glucose phosphate isomerase, and an unidentified acid serum protein. Considering the small number of founder specimens and subsequent inbreeding, allozyme variability appears fairly high in anoas. Genetic distances between zoo populations amount to 0.0505 or less. Southern blot hybridizations of restricted DNA from anoas and African buffaloes with a probe from the DRB-like region of the chimpanzee's MHC class II genes also indicate a low degree of genetic differentiation between mountain and lowland anoas. The relevance of these genetic data for the taxonomic classification of mountain and lowland anoas, and for the conservation of anoas by captive breeding is discussed. 相似文献