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Henning SM Niu Y Liu Y Lee NH Hara Y Thames GD Minutti RR Carpenter CL Wang H Heber D 《The Journal of nutritional biochemistry》2005,16(10):610-616
Tea polyphenols have strong in vitro antioxidant activity. Due to their limited bioavailability, however, their contribution to in vivo antioxidant activity may depend on the form of administration. A human intervention study was performed to evaluate the bioavailability and antioxidant capacity of (-)-epigallocatechin-3-gallate (EGCG) administered as a single large dose in the form of either purified EGCG or as green tea extract (Polyphenon E). Plasma concentrations of tea polyphenols were determined by high-performance liquid chromatography (HPLC) analysis combined with coulometric array electrochemical detection (ECD). We found no differences in plasma EGCG concentrations and trolox equivalents determined by the trolox equivalent antioxidant capacity assay after administration of either form of EGCG. However, we found that the plasma antioxidant activity was significantly affected by changes in the plasma urate concentration, which may have interfered with the effect of tea polyphenols on the antioxidant activity. In addition, lymphocyte 8-hydroxydeoxyguanosine to deoxyguanosine (8-OHdG/10(6)dG) ratios were determined by HPLC with ECD. The 8-OHdG/10(6)dG ratios did not change significantly during the 24 h following both EGCG interventions but correlated significantly within individuals determined during the two interventions separated by 1 week. In summary, changes in plasma uric acid due to dietary intake were significantly correlated to the plasma antioxidant activity and exerted a stronger influence on the plasma antioxidant activity compared with the EGCG intervention. In future studies of dietary effects on the plasma antioxidant capacity, changes in plasma uric acid will need to be closely monitored. 相似文献
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本文建立了酵母中麦角固醇含量高效液相色谱测定方法。其色谱条件为,色谱柱HYPersilBDSC185u反相柱,流动相为甲醇:水(97∶3),紫外检测波长为283nm。酵母样加碱乙醇皂化、提取、洗涤、蒸干、定量测定。结果表明:标准曲线范围是002—08mg/ml线性良好,最低限量为001mg/ml;日内及日间RSD(n=4)分别在21~40%和24~48%,回收率为960~980%。本方法操作简便、准确、可靠。 相似文献
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Hejing Yan Zhaozhe Hua Guoshi Qian Miao Wang Guocheng Du Jian Chen 《Biotechnology and Bioprocess Engineering》2009,14(3):354-360
In this paper the effect of cutinase on the degradation of cotton seed coat is analyzed. Fourier transform infrared (FT-IR)
microspectroscopy was applied to study the changes of chemical compositions in cotton seed coat epidermal layer and gas chromatography/mass
spectrometry (GC/MS) was used to analyse cutinase depolymerization of cotton seed coat. Based on these arguments the ability
of cutinase to degrade aliphatic components in cotton seed coat was verified. Positive effect of cutinase on degradation of
cotton seed coat was observed with the combination of alkaline pectinase or xylanase. The removal of aliphatic components
by cutinase enables other enzymes to penetrate into the inner of cotton seed coat. Cutinase can potentially improve the degradation
of cotton seed coat during cotton fabric bio-scouring process. 相似文献
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Hongxiang Ma Hejing Ge Xu Zhang Weizhong Lu Dazhao Yu He Chen Jianming Chen 《Journal of Phytopathology》2009,157(3):166-171
Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe is a devastating barley disease world-wide, causing significant yield losses and contaminating cereal products with mycotoxins. Barley grain contaminated with deoxynivalenol (DON) is associated with gushing and may be rejected by the malting and brewing industry. Genetically inherited resistance is the most effective option for the control of the disease. A total of 266 barley cultivars and breeding lines originating from China were evaluated for FHB resistance and concentration of DON in grain. Plants were inoculated with isolates of F. graminearum under field conditions by injecting conidia into a single spikelet of each spike. FHB symptoms were evaluated by visual inspection, and DON content was analysed by HPLC. Significant differences in FHB ratings and DON levels were observed among cultivars. Visual symptoms of FHB varied from 4.88 to 71.75% of infected spikelets 21 days after inoculation and from 7.86 to 113.33 area under the disease progress curve units (AUDPC). Twenty-seven lines were more resistant to FHB than the control resistant cultivar Zhedar 2 and with fewer than 12% infected spikelets. Twenty-one of the above lines originated from the area in the mid to low valley of Yangtze River, where FHB epidemics are frequent. DON levels ranged from 0.05 to 24.39 mg/kg among the tested barley lines. Correlation coefficients were significant between FHB symptom ratings and DON levels. However, there was no significant correlation between symptom rating and plant height and no significant correlation between symptom rating and heading date. 相似文献
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Liu Yi Liu Hejing Sheng Bo Pan Shuya Wang Zhi-wei Zhu Xueqiong 《Apoptosis : an international journal on programmed cell death》2022,27(9-10):685-696
Apoptosis - Cervical cancer is one of the most common female malignancies. Human papillomaviruses (HPV) are the main causative agents of virtually all cervical carcinomas. Nevertheless, emerging... 相似文献
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