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C Crone J Frokjaer-Jensen JJ Friedman O Christensen 《The Journal of general physiology》1978,71(2):195-220
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The in vitro proliferation and differentiation of myeloid progenitor cells (CFU-c) in agar culture from CBA/Ca mouse bone marrow cells was studied. Density sub-populations of marrow cells were obtained by equilibrium centrifugation in continuous albumin density gradients. The formation of colonies of granulocytes and/or macrophages was studied under the influence of three types of colony-stimulating factor (CSF) from mouse lung conditioned medium CSFMLCM), post-endotoxin mouse serum (CSFES) and from human urine (CSFHu). The effect of the sulphydryl reagent mercaptoethanol on colony development was also examined. The density distribution of CFU-c was dependent on the type of CSF. Functional heterogeneity was found among CFU-c with partial discrimination between progenitor cells forming pure granulocytic colonies and those forming pure macro-phage colonies. Mercaptoethanol increased colony incidence but had no apparent effect on colony morphology or the density distribution of CFU-c. 相似文献
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Delano V. Young Michael C. Dean Peter Heit Stewart D. Chipman 《In vitro cellular & developmental biology. Plant》1980,16(11):949-957
Summary Simian virus 40-transformed 3T3 cells are dependent on serum for survival and growth. This growth activity can be separated
on a pH 2 Sephadex G100 column into two fractions: a high molecular weight activity and a low molecular weight substance that
has recently been characterized as containing as its major agent, biotin.
To replace the remainder of the serum requirement, hormones and other growth factors were tested. Both insulin at high, nonphysiological
concentrations (200 to 500 ng/ml) and transferrin (5×10−8
M) stimulate the growth rate in low serum medium (0.3% v/v bovine calf serum DME) individually and, when added together, are
nearly as growth enhancing as 10% serum.
The need for the residual serum in this medium can be eliminated by the use of crystalline trypsin during trypsinization.
Under these serum-free conditions, biotin and transferrin supplementation provide for moderately good growth (20 to 30 hr
population doubling time, 1×106 cells/3.2-cm dish final cell density). Insulin addition further stimulates the growth rate (16 to 20 hr) and the final density
(1.5×106 cells). Although the protein growth factors, EGF (0.5 to 1.0 ng/ml) and FGF (4 to 10 ng/ml), also appear to enhance growth
individually and additively, their effects are slight and very variable. Nevertheless, the complete serum-free medium (DME
supplemented with biotin, transferrin, insulin, EGF and FGF) yields growth comparable but still inferior to 10% serum supplementation
(14-versus 12-hr population doubling time, 1 to 2×106 versus 2 to 3×106 cells final cell density).
This work was supported by NIH Grant CA 20040. 相似文献
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