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Mahdi Hesaraki Mojtaba Saadati Hossein Honari Gholamreza Olad Mohammad Heiat Fatemeh Malaei Reza Ranjbar 《Biologicals》2013,41(4):269-274
Shigella is known as pathogenic intestinal bacteria in high dispersion and pathogenic bacteria due to invasive plasmid antigen (Ipa). So far, a number of Ipa proteins have been studied to introduce a new candidate vaccine. Here, for the first time, we examined whether the N-terminal region of IpaD72–162 could be a proper candidate for Shigella vaccine. Initially, the DNA sequence coding N-terminal region was isolated by PCR from Shigella dysenteriae type I and cloned into pET-28a expression vector. Then, the heterologous protein was expressed, optimized and purified by affinity Ni–NTA column. Western blot analysis using, His-tag and IpaD72–162 polyclonal antibodies, confirmed the purity and specificity of the recombinant protein, respectively. Subsequently, the high immunogenicity of the antigen was shown by ELISA. The results of the sereny test in Guinea pigs showed that IpaD72–162 provides a protective system against Shigella flexneri 5a and S. dysenteriae type I. 相似文献
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Molecular Biology Reports - CYP24A1 plays a role in strictly regulated vitamin D metabolism pathway and has been nominated as a prognostic biomarker for colorectal cancer (CRC). Increasing evidence... 相似文献
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Hashemi Yeganeh Hamid Heiat Mohammad Alavian Seyed Moayed Rezaei Ehsan 《The protein journal》2022,41(4-5):527-542
The Protein Journal - Along with all cancer treatments, including chemotherapy, radiotherapy, and surgery, targeting therapy is a new treatment manner. Immunotoxins are new recombinant structures... 相似文献
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