排序方式: 共有113条查询结果,搜索用时 15 毫秒
1.
A YAC contig encompassing the Treacher Collins syndrome critical region at 5q31.3-32. 总被引:1,自引:1,他引:0 下载免费PDF全文
J. Dixon A. J. Gladwin S. K. Loftus J. H. Riley R. Perveen J. J. Wasmuth R. Anand M. J. Dixon 《American journal of human genetics》1994,55(2):372-378
Treacher Collins syndrome (TCOF1) is an autosomal dominant disorder of craniofacial development the features of which include conductive hearing loss and cleft palate. Previous studies have localized the TCOF1 locus between D5S519 (proximal) and SPARC (distal), a region of 22 centirays as estimated by radiation hybrid mapping. In the current investigation we have created a contig across the TCOF1 critical region, using YAC clones. Isolation of a novel short tandem repeat polymorphism corresponding to the end of one of the YACs has allowed us to reduce the size of the critical region to approximately 840 kb, which has been covered with three nonchimeric YACs. Restriction mapping has revealed that the region contains a high density of clustered rare-cutter restriction sites, suggesting that it may contain a number of different genes. The results of the present investigation have further allowed us to confirm that the RPS14 locus lies proximal to the critical region and can thereby be excluded from a role in the pathogenesis of TCOF1, while ANX6 lies within the TCOF1 critical region and remains a potential candidate for the mutated gene. 相似文献
2.
3.
Yousafi Qudsia Batool Jannat Khan Muhammad Saad Perveen Tamsila Sajid Muhammad Wasim Hussain Abrar Mehmood Asim Saleem Shahzad 《International journal of peptide research and therapeutics》2021,27(1):351-351
International Journal of Peptide Research and Therapeutics - The original version of the article unfortunately contained a typo in co-author name. 相似文献
4.
Fohad Mabood Husain Kahkashan Perveen Faizan Abul Qais Iqbal Ahmad Ahmed H. Alfarhan Mohamed A. El-Sheikh 《Saudi Journal of Biological Sciences》2021,28(1):333-341
Food producing animals harbouring bacteria carrying drug resistance genes especially the metallo-beta-lactamase (MBL) pose high risk for the human population. In addition, formation of biofilm by these drug resistant pathogens represents major threat to food safety and public health. In this study, metallo-β-lactamases (MβLs) producing Pseudomonas spp. from camel meat were isolated and assessed for their biofilm formation. Further, in vitro and in silico studies were performed to study the effect of flavone naringin on biofilm formation against isolated Pseudomonas spp. A total of 55% isolates were found to produce metallo-β-lactamase enzyme. Naringin mitigated biofilm formation of Pseudomonas isolates up to 57%. Disturbed biofilm architecture and reduced the colonization of bacteria on glass was observed under scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). The biofilm related traits such as exopolysaccharides (EPS) and alginate production was also reduced remarkably in the presence of naringin. Eradication of preformed biofilms (32–60%) was also observed at the respective 0.50 × MICs. Molecular docking revealed that naringin showed strong affinity towards docked proteins with binding energy ranging from −8.6 to −8.8 kcal mol−1. Presence of metallo-β-lactamase producers indicates that camel meat could be possible reservoir of drug-resistant Pseudomonas species of clinical importance. Naringin was successful in inhibiting biofilm formation as well as eradicating the preformed biofilms and demonstrated strong binding affinity towards biofilm associated protein. Thus, it is envisaged that naringin could be exploited as food preservative especially against the biofilm forming food-borne Pseudomonas species and is a promising prospect for the treatment of biofilm based infections. 相似文献
5.
Nighat Perveen Sabir Bin Muzaffar Mohammad Ali Al-Deeb 《Saudi Journal of Biological Sciences》2021,28(2):1417-1425
The novel coronavirus disease (COVID-19) that emerged in December 2019 had caused substantial morbidity and mortality at the global level within few months. It affected economies, stopped travel, and isolated individuals and populations around the world. Wildlife, especially bats, serve as reservoirs of coronaviruses from which the variant Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) emerged that causes COVID-19. In this review, we describe the current knowledge on COVID-19 and the significance of wildlife hosts in its emergence. Mammalian and avian coronaviruses have diverse host ranges with distinct lineages of coronaviruses. Recombination and reassortments occur more frequently in mixed-animal markets where diverse viral genotypes intermingle. Human coronaviruses have evolved through gene gains and losses primarily in interfaces where wildlife and humans come in frequent contact. There is a gap in our understanding of bats as reservoirs of coronaviruses and there is a misconception that bats periodically transmit coronaviruses to humans. Future research should investigate bat viral diversity and loads at interfaces between humans and bats. Furthermore, there is an urgent need to evaluate viral strains circulating in mixed animal markets, where the coronaviruses circulated before becoming adapted to humans. We propose and discuss a management intervention plan for COVID-19 and raise questions on the suitability of current containment plans. We anticipate that more virulent coronaviruses could emerge unless proper measures are taken to limit interactions between diverse wildlife and humans in wild animal markets. 相似文献
6.
A greenhouse experiment was conducted to examine the effect of foliar application of triacontanol (TRIA) on two cultivars (cv. S-24 and MH-97) of wheat (Triticum aestivum L.) at different growth stages. Plants were grown in full strength Hoagland’s nutrient solution under salt stress (150 mM NaCl) or control (0 mM NaCl) conditions. Three TRIA concentrations (0, 10, and 20 μM) were sprayed over leaves at three different growth stages, i.e. vegetative (V), boot (B), and vegetative + boot (VB) stages (two sprays on same plants, i.e., the first at 30-d-old plants and the second 78-d-old plants). Salt stress decreased significantly growth, net photosynthetic rate (P N), transpiration rate (E), chlorophyll contents (Chl a and b), and electron transport rate (ETR), while membrane permeability increased in both wheat cultivars. Stomatal conductance (g s) decreased only in salt-sensitive cv. MH-97 under saline conditions. Foliar application of TRIA at different growth stages enhanced significantly the growth, P N, g s, Chl a and b contents, and ETR, while membrane permeability was reduced in both cultivars under salt stress. Of various growth stages, foliar-applied TRIA was comparatively more effective when it was applied at V and VB stages. Overall, 10 μM TRIA concentration was the most efficient in reducing negative effects of salinity stress in both wheat cultivars. The cv. S-24 showed the better growth and ETR, while cv. MH-97 exhibited higher nonphotochemical quenching. 相似文献
7.
Yasmin Hasina Rahman Mohammed Shafikur Shibata Takayuki 《International journal of peptide research and therapeutics》2019,25(2):583-589
International Journal of Peptide Research and Therapeutics - Selective determination of peptides in mixtures or biological samples requires specific techniques for analysis. Herein, we report... 相似文献
8.
Arif Muhammad Rashid Naeem Perveen Sumera Bashir Qamar Akhtar Muhammad 《Extremophiles : life under extreme conditions》2019,23(1):69-77
Extremophiles - The gene-encoding Indole-3-glycerol phosphate synthase, a key enzyme involved in the cyclization of 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate, from Pyrococcus furiosus... 相似文献
9.
Saber Hamdani Hongru Wang Guangyong Zheng Shahnaz Perveen Mingnan Qu Naveed Khan Waqasuddin Khan Jianjun Jiang Ming Li Xinyu Liu Xiaocen Zhu Govindjee Chengcai Chu Xin‐Guang Zhu 《Physiologia plantarum》2019,166(1):105-119
The maximum quantum yield of photosystem II (as reflected by variable to maximum chlorophyll a fluorescence, Fv/Fm) is regarded as one of the most important photosynthetic parameters. The genetic basis underlying natural variation in Fv/Fm, which shows low level of variations in plants under non‐stress conditions, is not easy to be exploited using the conventional gene cloning approaches. Thus, in order to answer this question, we have followed another strategy: we used genome‐wide association study (GWAS) and transgenic analysis in a rice mini‐core collection. We report here that four single‐nucleotide polymorphisms, located in the promoter region of β‐glucosidase 5 (BGlu‐5), are associated with observed variation in Fv/Fm. Indeed, our transgenic analysis showed a good correlation between BGlu‐5 and Fv/Fm. Thus, our work demonstrates the feasibility of using GWAS to study natural variation in Fv/Fm, suggesting that cis‐element polymorphism, affecting the BGlu‐5 expression level, may, indirectly, contribute to Fv/Fm variation in rice through the gibberellin signaling pathway. Further research is needed to understand the mechanism of our novel observation. 相似文献
10.
Hamdani Saber Khan Naveed Perveen Shahnaz Qu Mingnan Jiang Jianjun Govindjee Zhu Xin-Guang 《Photosynthesis research》2019,139(1-3):107-121
Photosynthesis Research - Non-photochemical quenching (NPQ) of the excited state of chlorophyll a is a major photoprotective mechanism plants utilize to survive under high light. Here, we report... 相似文献