排序方式: 共有28条查询结果,搜索用时 15 毫秒
1.
2.
3.
Daniel Graf Paula M. Timmons Megan Hitchins Vasso Episkopou Gudrun Moore Takekito Ito Asao Fujiyama Amanda G. Fisher Matthias Merkenschlager 《Mammalian genome》2001,12(7):554-560
The twisted gastrulation gene (tsg) encodes a secreted protein required for the correct specification of dorsal midline cell fate during gastrulation in Drosophila. We report that tsg homologs from human, mouse, zebrafish, and Xenopus share 72–98% identity at the amino acid level and retain all 24 cysteine residues from Drosophila. In contrast to Drosophila where tsg expression is limited to early embryos, expression is found throughout mouse and human development. In Drosophila, tsg acts in synergy with decapentaplegic (dpp), a member of the TGF-β family of secreted proteins. The vertebrate orthologs of dpp, BMP-2 and -4, are crucial for gastrulation and neural induction, and aberrant signaling by BMPs and other TGF-β family members
results in developmental defects including holoprosencephaly (HPE). Interestingly, human TSG maps to the HPE4 locus on Chromosome
18p11.3, and our analysis places the gene within 5 Mbp of TG-interacting factor (TGIF).
Received: 21 August 2000 / Accepted: 9 March 2001 相似文献
4.
A L Manson A E Trezise L J MacVinish K D Kasschau N Birchall V Episkopou G Vassaux M J Evans W H Colledge A W Cuthbert C Huxley 《The EMBO journal》1997,16(14):4238-4249
We have made transgenic mice carrying a 320 kb YAC with the intact human cystic fibrosis transmembrane regulator (CFTR) gene. Mice that only express the human transgene were obtained by breeding with Cambridge null CF mice. One line has approximately two copies of the intact YAC. Mice carrying this transgene and expressing no mouse cftr appear normal and breed well, in marked contrast to the null mice, where 50% die by approximately 5 days after birth. The chloride secretory responses in these mice are as large or larger than in wild-type tissues. Expression of the transgene is highly cell type specific and matches that of the endogenous mouse gene in the crypt epithelia throughout the gut and in salivary gland tissue. However, there is no transgene expression in some tissues, such as the Brunner's glands, where it would be expected. Where there are differences between the mouse and human pattern of expression, the transgene follows the mouse pattern. We have thus defined a cloned fragment of DNA which directs physiological levels of expression in many of the specific cells where CFTR is normally expressed. 相似文献
5.
6.
Sox1 null lens fiber cells fail to elongate and have disrupted expression of gamma-crystallin. We have evaluated the expression of Sox1 and Pax6 proteins during critical stages of lens morphogenesis, with particular focus on fiber cell differentiation. While Pax6 and Sox1 are co-expressed during early stages of fiber cell differentiation, Sox1 up-regulation coincides temporally with the down-regulation of Pax6, and these proteins therefore display a striking inverse expression pattern in the lens fiber cell compartment. Furthermore, Pax6 is inappropriately expressed in the fiber cells of Sox1 null mice and the Pax6 target, alpha5 integrin, is simultaneously misexpressed. Finally, we demonstrate a genetic interaction between Sox1 and Pax6, as Sox1 heterozygosity partially rescues the diameter of Pax6(Sey) lenses by increasing the number of cells in the fiber cell compartment. 相似文献
7.
Theodora Manolakou Valeria Kaltezioti Niki Prakoura Panagiotis Kavvadas Simone Reichelt-Wurm Harikleia Gakiopoulou Miriam Banas Bernhard Banas Maja T. Lindenmeyer Clemens D. Cohen Peter Boor Sonja Djudjaj Dimitrios T. Boumpas Christos Chatziantoniou Aristidis Charonis Panagiotis K. Politis 《Journal of cellular and molecular medicine》2023,27(9):1192-1205
8.
9.
10.