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Transcriptome‐wide sequencing provides insights into geocarpy in peanut (Arachis hypogaea L.)
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Xiaoping Chen Qingli Yang Haifen Li Heying Li Yanbin Hong Lijuan Pan Na Chen Fanghe Zhu Xiaoyuan Chi Wei Zhu Mingna Chen Haiyan Liu Zhen Yang Erhua Zhang Tong Wang Ni Zhong Mian Wang Hong Liu Shijie Wen Xingyu Li Guiyuan Zhou Shaoxiong Li Hong Wu Rajeev Varshney Xuanqiang Liang Shanlin Yu 《Plant biotechnology journal》2016,14(5):1215-1224
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Li Yuanbin Liu Haifen Zeng Zhaohui Lin Hui Chen Xin Yuan Xianglian Qiu Jizhe Fu Fengchun Chen Zhuang Kuang Jianjun 《Journal of molecular histology》2022,53(4):763-772
Journal of Molecular Histology - We investigate the protective effect of ginsenoside Rb3 on skin flap microvasculature following ischemia-reperfusion (I/R) injury and its regulatory mechanism. We... 相似文献
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Boron and nitrogen co‐doped carbon dots as a sensitive fluorescent probe for the detection of curcumin
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Wei Bian Xuan Wang Yakun Wang Haifen Yang Jialin Huang Zongwei Cai Martin M. F. Choi 《Luminescence》2018,33(1):174-180
In this present study, a fluorescent probe was developed to detect curcumin, which is derived from the rhizomes of the turmeric. We used a simple and economical way to synthesize boron and nitrogen co‐doped carbon dots (BNCDs) by microwave heating. The maximum emission wavelength of the BNCDs was 450 nm at an excitation wavelength of 360 nm. The as‐prepared BNCDs were characterized by multiple analytical techniques such as transmission electron microscopy, X‐ray photoelectron spectroscopy, X‐ray diffraction, and infrared spectroscopy. The synthesized carbon nanoparticles had an average particle diameter of 4.23 nm. The BNCDs exhibited high sensitivity to the detection of curcumin at ambient conditions. The changes of BNCDs fluorescent intensity show a good linear relationship with the curcumin concentrations in the range 0.2–12.5 μM. This proposed method has been successfully applied to detect the curcumin in urine samples with the recoveries of 96.5–105.5%. 相似文献
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Shijie Wen Hao Liu Xingyu Li Xiaoping Chen Yanbin Hong Haifen Li Qing Lu Xuanqiang Liang 《Plant molecular biology》2018,97(1-2):177-185
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Tong Wang Xiaoping Chen Fanghe Zhu Haifen Li Ling Li Qingli Yang Xiaoyuan Chi Shanlin Yu Xuanqiang Liang 《PloS one》2013,8(4)
Background
Germin-like superfamily members are ubiquitously expressed in various plant species and play important roles in plant development and defense. Although several GLPs have been identified in peanut (Arachis hypogaea L.), their roles in development and defense remain unknown. In this research, we study the spatiotemporal expression of AhGLPs in peanut and their functions in plant defense.Results
We have identified three new AhGLP members (AhGLP3b, AhGLP5b and AhGLP7b) that have distinct but very closely related DNA sequences. The spatial and temporal expression profiles revealed that each peanut GLP gene has its distinct expression pattern in various tissues and developmental stages. This suggests that these genes all have their distinct roles in peanut development. Subcellular location analysis demonstrated that AhGLP2 and 5 undergo a protein transport process after synthesis. The expression of all AhGLPs increased in responding to Aspergillus flavus infection, suggesting AhGLPs'' ubiquitous roles in defense to A. flavus. Each AhGLP gene had its unique response to various abiotic stresses (including salt, H2O2 stress and wound), biotic stresses (including leaf spot, mosaic and rust) and plant hormone stimulations (including SA and ABA treatments). These results indicate that AhGLPs have their distinct roles in plant defense. Moreover, in vivo study of AhGLP transgenic Arabidopsis showed that both AhGLP2 and 3 had salt tolerance, which made transgenic Arabidopsis grow well under 100 mM NaCl stress.Conclusions
For the first time, our study analyzes the AhGLP gene expression profiles in peanut and reveals their roles under various stresses. These results provide an insight into the developmental and defensive roles of GLP gene family in peanut. 相似文献8.
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在Tv ALP基因和Tv RBL基因间加入一段柔性链接头(linker)基因序列,构建融合基因。生物信息学分析表明该融合基因含有一段信号肽序列。利用RT-PCR技术从绿色木霉菌丝体总RNA中扩增出Tv RBL基因、Tv ALP基因和去除信号肽的ΔTvALP基因,分别克隆到原核表达载体p ET30a,构建重组质粒p ET30a-Tv ALP-linker-Tv RBL和p ET30a-ΔTv ALP-linker-Tv RBL,转化大肠杆菌BL21(DE3)p Lys S,经异丙基硫代-β-D-半乳糖苷(IPTG)诱导表达。SDS-PAGE电泳分析结果表明,去除信号肽的表达质粒p ET30a-ΔTv ALP-linker-Tv RBL在大肠杆菌BL21(DE3)p Lys S中获得了表达,在60 k D处有一条蛋白质特异条带,与预测的目的产物蛋白条带大小一致。 相似文献
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