排序方式: 共有17条查询结果,搜索用时 15 毫秒
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ROMAIN Guiho KEVIN BITEAU GIULIA GRISENDI MATHIAS CHATELAIS REGIS BRION JULIEN TAURELLE SARAH RENAULT DOMINIQUE HEYMANN MASSIMO DOMINICI FRANÇOISE REDINI 《Cytotherapy》2018,20(8):1037-1045
Background
Osteosarcoma (OS) is the most frequent pediatric malignant bone tumor. OS patients have not seen any major therapeutic progress in the last 30 years, in particular in the case of metastatic disease, which requires new therapeutic strategies. The pro-apoptotic cytokine Tumor necrosis factor (TNF)–Related Apoptosis Inducing Ligand (TRAIL) can selectively kill tumor cells while sparing normal cells, making it a promising therapeutic tool in several types of cancer. However, many OS cell lines appear resistant to recombinant human (rh)TRAIL-induced apoptosis. We, therefore, hypothesized that TRAIL presentation at the membrane level of carrier cells might overcome this resistance and trigger apoptosis.Methods
To address this, human adipose mesenchymal stromal cells (MSCs) transfected in a stable manner to express membrane-bound full-length human TRAIL (mbTRAIL) were co-cultured with several human OS cell lines.Results
This induced apoptosis by cell-to-cell contact even in cell lines initially resistant to rhTRAIL. In contrast, mbTRAIL delivered by MSCs was not able to counteract tumor progression in this OS orthotopic model.Discussion
This was partly due to the fact that MSCs showed a potential to support tumor development. Moreover, the expression of mbTRAIL did not show caspase activation in adjacent tumor cells. 相似文献5.
ANNE-LISE VARNIER LISA SANCHEZ PARUL VATSA LESLIE BOUDESOCQUE ANGELA GARCIA-BRUGGER FANJA RABENOELINA ALEXANDER SOROKIN JEAN-HUGUES RENAULT SERGE KAUFFMANN ALAIN PUGIN CHRISTOPHE CLEMENT FABIENNE BAILLIEUL & STEPHAN DOREY 《Plant, cell & environment》2009,32(2):178-193
Rhamnolipids produced by the bacteria Pseudomonas aeruginosa are known as very efficient biosurfactant molecules. They are used for a wide range of industrial applications, especially in food, cosmetics and pharmaceutical formulations as well as in bioremediation of pollutants. In this paper, the role of rhamnolipids as novel molecules triggering defence responses and protection against the fungus Botrytis cinerea in grapevine is presented. The effect of rhamnolipids was assessed in grapevine using cell suspension cultures and vitro-plantlets. Ca2+ influx, mitogen-activated protein kinase activation and reactive oxygen species production form part of early signalling events leading from perception of rhamnolipids to the induction of plant defences that include expression of a wide range of defence genes and a hypersensitive response (HR)-like response. In addition, rhamnolipids potentiated defence responses induced by the chitosan elicitor and by the culture filtrate of B. cinerea . We also demonstrated that rhamnolipids have direct antifungal properties by inhibiting spore germination and mycelium growth of B. cinerea . Ultimately, rhamnolipids efficiently protected grapevine against the fungus. We propose that rhamnolipids are acting as microbe-associated molecular patterns (MAMPs) in grapevine and that the combination of rhamnolipid effects could participate in grapevine protection against grey mould disease. 相似文献
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JULIEN PÉTILLON KEVIN LAMBEETS BRUNILDE RACT‐MADOUX PHILIPPE VERNON DAVID RENAULT 《Physiological Entomology》2011,36(2):165-172
Salinity interacts with many physiological functions and therefore probably influences the distribution of terrestrial fauna in tidal flooded salt marshes. The present study tests the hypothesis that the physiological tolerance of stenotopic wolf spiders for saline conditions at least partially determines their occurrence throughout salt‐marsh and nonsaline habitats. The duration of survival of three stenotopic wolf spider species (Araneae: Lycosidae) with different habitat preferences is compared in a controlled laboratory experiment. The forest‐dwelling Pardosa saltans, the salt‐marsh resident Pardosa purbeckensis and its sister species the inland‐living Pardosa agrestis are exposed to experimental conditions with different levels of salinity. Individuals (45 males and 20–45 females per treatment) are placed in individual air‐tight boxes filled with water‐saturated sand. Three levels of salinity are tested: nonsaline (0‰), medium saline (33–35‰) and highly saline (66–70‰). Contents of carbon, hydrogen and nitrogen and the molar ration carbon/nitrogen remain constant over time and do not differ among salinity treatments, indicating that starvation effects on survival, if any, are similar for all treatments. Conversely, body water significantly decreases over time and differs among salinity treatments, in accordance with patterns of survival. Conforming to their habitat preference, the survival of P. saltans and P. agrestis decreases quickly under highly saline conditions. Pardosa purbeckensis, however, has a high survival under both saline and nonsaline conditions. The duration of survival of females is significantly lower than that of males of P. saltans and P. purbeckensis. Durations of survival of ground‐living wolf spiders exposed to salinity partly match their habitat distribution but do not explain the restriction of salt‐marsh species to saline habitats. 相似文献
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本文研究了升高环境温度对小粉虫(Alphitobiusdiaperinus,鞘翅目拟步行虫总科)末龄幼虫、蛹以及成虫的失水和热敏性(临界热极值,Criticalthermalmaximum,CTmax)的影响。小粉虫成虫和蛹的CTmax值显著低于末龄幼虫(末龄幼虫:CTmax=48.5±0.5℃;蛹:CTmax=48.0±0.9℃;成虫:CTmax=47.8±1.1℃)。此外,成虫的适应性对其CTmax没有显著的影响。在20至60℃连续记录失水值中,三个龄期之间存在显著差异。随着超过临界点温度(约为40℃)的快速升高,末龄幼虫与成虫的失水程度相近。蛹具有明显低的失水速率,即使在温度高于40℃的情况下。当温度升高10℃时(25-35℃),会造成小粉虫蛹和成虫失水增加2.0倍和2.6倍,分别为:Q10=2.05±0.70和2.49±1.31,而幼虫则为3.52±1.27。当高于35-45℃时,幼虫和成虫失水增加甚快(Q10=6.85±1.90和8.51±2.32)。而蛹在高于35-45℃时失水也增加(Q10=3.76±1.83),但低于幼虫和成虫。小粉虫的蛹处于静止和不取食状态,代谢速率较低,其具有特殊结构的气孔能较好地保持关闭状态,且蛹的表皮由脂肪和蛋白质组成,以上诸因素可解释其失水减少的原因 相似文献