Ontogeny of Mouse Liver Peroxisomes and Catalase Isozymes

PEROXISOMES are cytoplasmic organelles which occur in liver and kidney cells of higher animals and in lower forms of life. They have a unique enzyme composition and function in the oxidation of specific substrates by oxidases¹. Catalase (hydrogen peroxide: hydrogen peroxide oxidoreductase E.C. 1.11.1.6) is an essential component of this oxidizing system which facilitates the catalytic or peroxidatic destruction of hydrogen peroxide. Large granular catalase activity serves as a marker for the organelle and has been used here to describe the ontogeny of peroxisomes in mouse liver. The results indicate that bursts of peroxisomal synthesis occur during the development of the mouse liver, particularly in the early postnatal stages and during maturation.     

Effects of Fusarium nivale and F. culmorum on the establishment of four species of pasture grass

Cocksfoot cv. Aberystwyth S 26 and timothy cv. Scots were found to be significantly more susceptible to pre-emergence death caused by Fusarium nivale and F. culmorum than were either perennial ryegrass cv. Gremie, or Italian ryegrass cv. RvP. The extent to which each grass was affected by Fusarium at the pre-emergence stage was markedly influenced by both the air temperature and the level of inoculum present in the soil at the time the seeds were sown. Under all conditions, however, cocksfoot and timothy suffered greater reductions in emergence when sown in soil containing either species of Fusarium than did the ryegrasses.
Evidence is presented which suggests that the extreme susceptibility of cocksfoot and timothy to pre-emergence damage is related to the slow germination of the former and the small seed size of the latter.
In addition to their direct effects on seedling numbers both fungi reduced the vigour of those seedlings which did emerge, cocksfoot and timothy being particularly badly affected.     

Patterns of Root Colonization in Epacridaceous Plants Collected from Different Sites

Root colonization was studied in ten species of the Epacridaceaeat three sites in Victoria by morphological and cross-inoculationexperiments. The sites and genera chosen were Cranbourne [Epacrisimpressa Labill. andLeucopogon ericoides(Smith) R. Br.] andRye [L. parviflorus(Andrews) Lindley] on the Mornington Peninsula,and the Grampians[Astroloma conostephioides(Sond.) Benth.,A.humifusum(Cav.) R. Br.,A pinifolium(R. Br.) Benth,Brachylomadaphnoides(Smith) Benth.,E. impressa, E. impressavar.grandifloraBenth.andStyphelia adscendensR. Br.] in western Victoria. For morphologicalstudies, samples of roots from each species at each site werecleared and stained and examined microscopically. For cross-inoculationstudies, cuttings from each site were struck in potting mediuminoculated with soil from the same and other sites. The ericoidmycorrhizae in the roots of plants found at or grown in Cranbourneand Rye soils were similar. Both were significantly differentfrom the internal hyphae found in the roots of plants foundat or grown in Grampians soils, which were three times largerin diameter and formed dense coils which filled the host celland invaded adjacent epidermal cells. This suggests that morethan one fungus is involved in the relationships, that the MorningtonPeninsula sites had a different fungus from the Grampians siteand that host specificity is low. Vesicular structures werealso found commonly on plants at the Grampians site, in contrastwith other sites. Epacridaceae; root; fungus; mycorrhiza; morphology; inoculation     

An analysis of nucleotide and amino acid variability in the barcode region of cytochrome c oxidase I (cox1) in fishes

The 655 bp cytochrome c oxidase subunit I barcode region of single specimens of 388 species of fishes (four Holocephali, 61 Elasmobranchii and 323 Actinopterygii) was examined. All but two (Urolophus cruciatus and Urolophus sufflavus) showed different cox1 nucleotide sequences (99.5% species discrimination); the two that could not be resolved are suspected to hybridize. Most of the power of cox1 nucleotide sequence analysis for species identification comes from the degenerate nature of the genetic code and the highly variable nature of the third codon position of amino acids. Variation at the third codon position is bimodally distributed, and the more variable mode is dominated by amino acids with four or six codons, while the less variable mode is dominated by amino acids with two codons. The ratio of nonsynonymous to synomymous changes is much less than one, indicating that this gene is subject to strong purifying selection. Consequently, cox1 amino acid sequence diversity is much less than nucleotide sequence diversity and has very poor species resolution power. Fourteen of the 16 amino acid residues recognized as having important functions in the region of cox1 sequenced were completely conserved over all 388 species (and the bovine cox1 sequence), with one fish species varying at one of these sites, and three fish at another site. No significant differences in amino acid conservation were observed between residues in helices, strands and turns. Patterns of nucleotide and amino acid variability were very similar between elasmobranchs and actinopterygians.     

Ultrasound imaging in an experimental model of fatty liver disease and cirrhosis in rats

Background

Atypical scrapie was first identified in Norwegian sheep in 1998 and has subsequently been identified in many countries. Retrospective studies have identified cases predating the initial identification of this form of scrapie, and epidemiological studies have indicated that it does not conform to the behaviour of an infectious disease, giving rise to the hypothesis that it represents spontaneous disease. However, atypical scrapie isolates have been shown to be infectious experimentally, through intracerebral inoculation in transgenic mice and sheep. The first successful challenge of a sheep with 'field' atypical scrapie from an homologous donor sheep was reported in 2007.

Results

This study demonstrates that atypical scrapie has distinct clinical, pathological and biochemical characteristics which are maintained on transmission and sub-passage, and which are distinct from other strains of transmissible spongiform encephalopathies in the same host genotype.

Conclusions

Atypical scrapie is consistently transmissible within AHQ homozygous sheep, and the disease phenotype is preserved on sub-passage.     

Mapping of citrullinated fibrinogen B-cell epitopes in rheumatoid arthritis by imaging surface plasmon resonance

Introduction  

Rheumatoid arthritis (RA) frequently involves the loss of tolerance to citrullinated antigens, which may play a role in pathogenicity. Citrullinated fibrinogen is commonly found in inflamed synovial tissue and is a frequent target of autoantibodies in RA patients. To obtain insight into the B-cell response to citrullinated fibrinogen in RA, its autoepitopes were systematically mapped using a new methodology.     

Off-Highway Vehicle Trail Impacts on Breeding Songbirds in Northeastern California

Abstract: Rapid growth in off-highway vehicle (OHV) use in North America leads to concerns about potential impacts on wildlife populations. We studied the relationship between distance to active OHV trail and songbird nesting success and abundance in northeastern California, USA, from 2002 to 2004. We found evidence of greater nest desertion and abandonment and reduced predation on shrub nests <100 m from OHV trails than at nests >100 m from OHV trails. Two of 18 species studied were less abundant at sites on trails than at sites 250 m from trails, and no species were more abundant on trails. Management of OHV trail development should consider possible negative impacts on nesting success and abundance of breeding birds.