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Background
Micro-biological research relies on the use of model organisms that act as representatives of their species or subspecies, these are frequently well-characterized laboratory strains. However, it has often become apparent that the model strain initially chosen does not represent important features of the species. For micro-organisms, the diversity of their genomes is such that even the best possible choice of initial strain for sequencing may not assure that the genome obtained adequately represents the species. To acquire information about a species' genome as efficiently as possible, we require a method to choose strains for analysis on the basis of how well they represent the species. 相似文献3.
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S SPIEGEL D HOLLAND Y TAM I BAR-YAAKOV L MASLENIN A ROSNER 《The Annals of applied biology》2004,144(2):229-236
Prunus necrotic ringspot virus (PNRSV) was detected in almonds, plum and apricot germplasm accessions and local almond cultivars in Israel. PNRSV was widespread both in wild and cultivated almond trees and uncommon in wild apricots and plums. The possible variation among the PNRSV isolates was initially evaluated by restriction analysis of PCR products representing the CP gene with the endonuclease RsaI and followed by nucleotide sequence analysis of selected isolates. It was concluded that all 13 isolates belong to group PV96, the largest cluster of PNRSV isolates, described previously. Two PNRSV isolates, one from a plum accession and one from an almond cultivar, were found to be distinct members of group PV96 with unique nucleotide modifications not found in other documented isolates of this virus. However, no PNRSV isolate typical to a specific host and/or to the Middle East region could be identified. This study expands the body of data on variability of PNRSV isolates and highlights the importance of assessing the virus status of germplasm collections by applying reliable diagnostic and differentiating methods. 相似文献
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Arable production has come under increasing economic and environmental pressures, especially in the last decade. These have derived from over-production, decreased farm incomes and a concern with the possible environmental effects of intensive pesticide use associated with such intensive cultivation. A number of long-term research programmes on integrated farming systems and their sustainability have recently been completed or are currently under way. In the UK, these include the ‘Boxworth’ project, ‘SCARAB’, TALISMAN’, RISC, ‘LINK Integrated Farming Systems’, ‘LIFE’ and the demonstration-only programme ‘LEAF’. These projects are reviewed in terms of their objectives, designs and results to date, and are compared with some parallel programmes in Germany, the Netherlands, Switzerland and France. 相似文献
6.
CHARLES H. HOLLAND 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1978,11(1):85-90
Quantitative treatment of the geological sciences must remain limited; there continues to be need for a language of words. This is keenly felt in stratigraphy, where the practising geologist is dismayed by the effusions of the stratigraphical philosophers. The situation, in so far as it appears to remain in some ways obscure or troublesome, is briefly reviewed in terms of lithostratigraphy. biostratigraphy. and chronostratigraphy. A revised version of a controversial diagram is presented, in which the role of the boundary stratotype as anchor point is believed to be clarified. Finally a synthesis is attempted of relationships between the various stratigraphical procedures and the provision of dates in years through the parallel discipline of geochronometry. 相似文献
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The distribution of the freshwater Malacostraca in the area of the Mersey and Weaver River Authority 总被引:1,自引:0,他引:1
D. G. HOLLAND 《Freshwater Biology》1976,6(3):265-276
Freshwater Malacostraca distribution data acquired through the routine biological work of a river authority is of general scientific interest. The distribution of Asellus aquaticus and Gammarus pulex is related to pollution and natural factors. Although widespread in the area, both species are absent from an extensive part of the major river system. A. meridianus appears to be less successful in competition with A. aquaticus and is comparatively scarce, being mostly limited to isolated pools. The distribution of Crangonyx pseudogracilis in canals and static waters is also related to pollution and natural factors. Orchestia cavimana, a semi-terrestrial amphipod, is shown to be an overlooked species which is common in canals. 相似文献
8.
Different values have resulted in conflicts between anglers and conservation lobbies in the management of trout in South Africa. Key to the conflict is the demarcation of boundaries to areas in which brown trout Salmo trutta and rainbow trout Oncorhynchus mykiss currently occur, or are likely to establish following stocking for angling. To provide a longer-term perspective on these areas, we developed models to link salmonid biological thermal thresholds to elevation. These, when applied spatially using a digital elevation model with a probability of occurrence model, provided the basis for estimating potentially available thermal habitat for these two cold water species. Here, we acknowledge that other variables (stocking history; river connectivity) also play a role in understanding trout distributions. Using a simple scenario of an increase in mean daily water temperatures of 2 °C, we demonstrated that both brown and rainbow trout are likely to exhibit considerable range reductions in the future. Because it is possible that these range restrictions will result in an increasing desire to introduce trout into areas above their current distribution limits for the maintenance of angling opportunities, conservation managers should prioritise these areas, with management interventions seeking to understand what will help to limit introductions. 相似文献
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LYNN RANÅKER MIKAEL JÖNSSON P. ANDERS NILSSON CHRISTER BRÖNMARK 《Freshwater Biology》2012,57(9):1761-1768
1. Environmental changes such as eutrophication and increasing inputs of humic matter (brownification) may have strong effects on predator–prey interactions in lakes through a reduction in the visual conditions affecting foraging behaviour of visually oriented predators. 2. In this experiment, we studied the effects of visual range (25–200 cm) in combination with optically deteriorating treatments (algae, clay or brown humic water) on predator–prey interactions between pike (Esox lucius) and roach (Rutilus rutilus). We measured effects on reaction distance and strike distance for pike and escape distance for roach, when pike individuals were exposed to free‐swimming roach as well as to roach held in a glass cylinder. 3. We found that reaction distance decreased with decreasing visual range caused by increasing levels of algae, clay or humic matter. The effect of reaction distance was stronger in turbid water (clay, algae) than in the brown water treatment. 4. Strike distance was neither affected by visual range nor by optical treatment, but we found shorter strike distances when pike attacked roach using visual cues only (roach held in a cylinder) compared to when pike could use multiple senses (free‐swimming roach). Escape distance for roach was longer in turbid than in brown water treatments. 5. Changes in environmental drivers, such as eutrophication and brownification, affecting the optical climate should thus have consequences for the strength of predator–prey interactions through changes in piscivore foraging efficiency and prey escape behaviour. This in turn may affect lake ecosystems through higher‐order interactions. 相似文献
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