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Multiple isoforms of UDP-GalNAc:polypeptide N-acetylgalactosaminyl-
transferase (ppGaNTase) have been cloned and expressed from a variety of
organisms. In general, these isoforms display different patterns of
tissue-specific expression, but exhibit overlapping substrate
specificities, in vitro . A peptide substrate, derived from the sequence of
the V3 loop of the HIV gp120 protein (HIV peptide), has previously been
shown to be glycosylated in vitro exclusively by the ppGaNTase-T3 (Bennett
et al. , 1996). To determine if this isoform- specificity is maintained in
vivo , we have examined the glycosylation of this substrate when it is
expressed as a reporter peptide (rHIV) in a cell background (COS7 cells)
which lacks detectable levels of the ppGaNTase-T3. Glycosylation of rHIV
was greatly increased by coexpression of a recombinant ppGaNTase-T3.
Overexpression of ppGaNTase- T1 yielded only partial glycosylation of the
reporter. We have also determined that the introduction of a proline
residue at the +3 position flanking the potential glycosylation site
eliminated ppGaNTase- T3 selectivity toward rHIV observed both in vivo and
in vitro .
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A Specific Light Chain of Kinesin Associates with Mitochondria in Cultured Cells 总被引:12,自引:2,他引:10
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Alexey Khodjakov Ekaterina M. Lizunova Alexander A. Minin Michael P. Koonce Fatima K. Gyoeva 《Molecular biology of the cell》1998,9(2):333-343
The motor protein kinesin is implicated in the intracellular transport of organelles along microtubules. Kinesin light chains (KLCs) have been suggested to mediate the selective binding of kinesin to its cargo. To test this hypothesis, we isolated KLC cDNA clones from a CHO-K1 expression library. Using sequence analysis, they were found to encode five distinct isoforms of KLCs. The primary region of variability lies at the carboxyl termini, which were identical or highly homologous to carboxyl-terminal regions of rat KLC B and C, human KLCs, sea urchin KLC isoforms 1–3, and squid KLCs. To examine whether the KLC isoforms associate with different cytoplasmic organelles, we made an antibody specific for a 10-amino acid sequence unique to B and C isoforms. In an indirect immunofluorescence assay, this antibody specifically labeled mitochondria in cultured CV-1 cells and human skin fibroblasts. On Western blots of total cell homogenates, it recognized a single KLC isoform, which copurified with mitochondria. Taken together, these data indicate a specific association of a particular KLC (B type) with mitochondria, revealing that different KLC isoforms can target kinesin to different cargoes. 相似文献
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F. K. Gyoeva 《Molecular Biology》2005,39(4):614-622
This review is devoted to the problem of regulation of intracellular organelle transport along microtubules. Properties of the system of microtubules as transport tracks are considered. Some motor proteins (kinesins and dyneins) carrying their “cargoes” in opposite directions are characterized. Special attention is paid to the analysis of transport of the organelles that can change the direction of movement. Possible molecular mechanisms coordinating the functioning of opposite motor proteins are considered.__________Translated from Molekulyarnaya Biologiya, Vol. 39, No. 4, 2005, pp. 709–718.Original Russian Text Copyright © 2005 by Gyoeva.I dedicate this review to the memory of Andrei Nikolaevich Belozerskii. I had no opportunity to become his disciple because he had already passed away when I came to the Department of Plant Biochemistry, Biological Faculty, Moscow State University. Nevertheless, my education is indebted to the Department that he headed for a long time and whose life was charged with the charm of his personality. 相似文献
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F. K. Gyoeva 《Biochemistry. Biokhimii?a》2014,79(9):849-855
The signaling and transport systems of eucaryotic cells are tightly interconnected: intracellular transport along microtubules and microfilaments is required to position signaling-pathway components, while signaling molecules control activity of motor proteins and their interaction with tracks and cargoes. Recent data, however, give evidence that active transport is engaged in signaling as a means of signal transduction. This review focuses on this specific aspect of the interaction of two systems. 相似文献
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Microtubule-associated proteins and microtubule-based translocators have different binding sites on tubulin molecule 总被引:9,自引:0,他引:9
V I Rodionov F K Gyoeva A S Kashina S A Kuznetsov V I Gelfand 《The Journal of biological chemistry》1990,265(10):5702-5707
It has been previously shown that a class of microtubule proteins, the so-called microtubule-associated proteins (MAPs), binds to the C-terminal part of tubulin subunits. We show here that microtubules composed of tubulin whose 4-kDa C-terminal domain was cleaved by subtilisin (S-microtubules) are unable to bind MAPs but can still bind the anterograde translocator protein kinesin and the retrograde translocator dynein. Binding of both motors to S-microtubules, like their binding to normal microtubules, was ATP-dependent. In addition, direct competition experiments showed that binding sites for kiensin and MAPs on the microtubule surface lattice do not overlap. Furthermore, S-microtubules stimulated the ATPase activity of kinesin at least 8-fold, and the affinities of kinesin for control and S-microtubules were identical. S-microtubules were able to glide along kinesin-coated coverslips at a rate of 0.2 microns/s, the same rate as control microtubules. We conclude, that unlike MAPs, kinesin and cytoplasmic dynein bind to the tubulin molecule outside the C-terminal region. 相似文献
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Conventional kinesin is a multifunctional motor protein that transports numerous organelles along microtubules. The specificity of kinesin-cargo binding is thought to depend on the type(s) of light chains that a kinesin molecule contains. We have shown previously that different isoforms of kinesin light chains are associated with different types of cargo, mitochondria and membranes of the Golgi complex. Here, we provide evidence that the two light chains present within each kinesin molecule are always of the same type. Further, we demonstrate that kinesin heavy chains interact with nascent light-chain polypeptides on ribosomes. These data suggest that incorporation of the two identical light chains into a single kinesin molecule most likely occurs cotranslationally. 相似文献
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