Inositol tetrakisphosphates as second messengers induce Ca(++)-dependent chloride currents in Xenopus laevis oocytes.

Microinjection of inositol 1,3,4,5-tetrakisphosphate or inositol 1,4,5-trisphosphate induced distinct chloride membrane currents in defolliculated Xenopus laevis oocytes. To decide whether these Cl(-)-currents were due to the injected compounds or their metabolic products, [3H]Ins(1,3,4,5)P4 or [3H]Ins(1,4,5)P3 were injected into oocytes and their metabolites were analyzed by HPLC. Our results indicate that Ins(1,3,4,5)P4 itself or its metabolite Ins(1,3,4,6)P4 is able to induce Cl(-)-membrane currents, most likely by increasing the cytosolic Ca(++)-concentration.     

Lymphatic bed of the muscular sheath of the small intestine in animals and man during ontogenesis]

In the anatomy of the lymphatic bed of the small intestine muscle layer still there are obscure questions on the phylo- and ontogenesis of man and animals. Under study were 92 corpses (35 men, 27 cats and 30 dogs) of different age, beginning from the intrauterine period to old age. Different methods were used: polychromatic injection; macro- and microscopic dissection; staining after van Gieson and with haematoxilineeosin; impregnation with 0,25-1% solution of silver nitrate; dehydration and clearing; counting of the density of loops per a mm2 and the depth of their disposition in the intestinal wall with an ocular-micrometer. It was shown that formation of the lymph capillaries and their network in cats, dogs and man began from the end of the intrauterine period, was completed in new-borns and became sufficiently developed in young age. The structure of the lymph capillary networks is closely connected with the development of the muscle layer of the small intestine. Formation of the lymph lacunas begins after birth. With age the lymph capillary network becomes looser, the loops break and their size enlarges.     

Change in the plasmocytic reaction of immunized animals during the use of antibiotics]

The effect of tetracycline and tetraolean on the shifts in the plasma-cell elements was studied experimentally on male albino rats of Wister line and rabbits immunized with foreign erythrocytes and typhoid fever vaccine respectively. It was noted that prolonged use of the antibiotics for 10 to 12 days inhibited proliferation of the plasmic cells, the inhibitory effect of tetracycline being more pronounced. At the same time an inhibitory effect of the antibiotics on the antibody formation in the animals was found in the experiments.     

Affinity of Ionic Species of Nucleoside Transport Protein Inhibitors

Abstract

A class of very potent nucleoside transport inhibitors is present in two molecular forms around physiological pH. We investigated whether the monoprotonated or the unionized species of these molecules binds to this camer protein with higher affinity.     

THE EFFECTS OF INJURY PREVENTION WARM-UP PROGRAMMES ON KNEE STRENGTH IN MALE SOCCER PLAYERS

The study investigates the effects of the 11+ and HarmoKnee injury prevention programmes on knee strength in male soccer players. Under-21-year-old players (n=36) were divided equally into: the 11+, HarmoKnee and control groups. The programmes were performed for 24 sessions (20-25 min each). The hamstrings and quadriceps strength were measured bilaterally at 60°·s^-1, 180°·s^-1 and 300°·s^-1. The concentric quadriceps peak torque (PT) of the 11+ increased by 27.7% at 300°·s^-1 in the dominant leg (p<0.05). The concentric quadriceps PT of HarmoKnee increased by 36.6%, 36.2% and 28% in the dominant leg, and by 31.3%, 31.7% and 20.05% at 60°·s^-1, 180°·s^-1 and 300°·s^-1 in the non-dominant leg respectively. In the 11+ group the concentric hamstring PT increased by 22%, 21.4% and 22.1% at 60°·s^-1, 180°·s^-1 and 300°·s^-1, respectively in the dominant leg, and by 22.3%, and 15.7% at 60°·s^-1 and 180°·s^-1, in the non-dominant leg. In the HarmoKnee group the hamstrings in the dominant leg showed an increase in PT by 32.5%, 31.3% and 14.3% at 60°·s^-1, 180°·s^-1 and 300°·s^-1, and in the non-dominant leg hamstrings PT increased by 21.1% and 19.3% at 60°·s^-1 and 180°·s^-1 respectively. The concentric hamstrings strength was significantly different between the 11+ and control groups in the dominant (p=0.01) and non-dominant legs (p=0.02). The HarmoKnee programme enhanced the concentric strength of quadriceps. The 11+ and HarmoKnee programmes are useful warm-up protocols for improving concentric hamstring strength in young professional male soccer players. The 11+ programme is more advantageous for its greater concentric hamstring strength improvement compared to the HarmoKnee programme.     

P2X7-mediated ATP secretion is accompanied by depletion of cytosolic ATP

Purinergic Signalling - ATP and its metabolites are important extracellular signal transmitters acting on purinergic P2 and P1 receptors. Most cells can actively secrete ATP in response to a...