油菜基因流网络的结构特性与小团体分析

为从系统角度掌握转基因油菜(Brassica campestris)基因流规律, 基于常规油菜与十字花科植物杂交的有关文献,构建了油菜基因流网络拓扑图实例并分析其网络结构特性。研究结果表明, 该网络节点度服从幂律分布, 具有无标度特性。从随机攻击和恶性攻击两个方面对标准结构熵和网络效率2个指标的网络稳健性进行分析, 结果显示在随机移除不到10%的顶点时网络表现较好的鲁棒性, 但在受到选择性移除10%的顶点时, 网络具有极弱的抗攻击性。基于软件UCINET对网络进行了小团体和结构同型性分析, 可将网络中298个节点22类十字花科植物划分为2个小团体和5类结构角色, 其中甘蓝型油菜在基因流网络小团体中具有关键性作用。这些研究结果可为揭示转基因油菜基因流规律提供新思路, 同时也可对转基因油菜商业化种植采取合理的农业生产栽培管理措施提供参考。     

柑桔园生草栽培的生态效应研究

柑桔园生草栽培的生态效应研究李国怀章文才刘继红胡德文（华中农业大学园艺系,武汉４３００７０）ＳｔｕｄｉｅｓｏｆＥｃｏｌｏｇｉｃａｌＥｆｅｃｔｓｏｆＳｏｄＣｕｌｔｕｒｅｉｎＣｉｔｒｕｓＯｒｃｈａｒｄ．ＬｉＧｕｏｈｕａｉ,ＺｈａｎｇＷｅｎｃａｉ,ＬｉｕＪ...     

板栗的开花结果习性与性别表现

通过对罗田板栗早栗,中迟栗和羊毛栗等中迟栗和羊毛栗等开花结果习性及性别表现的观察研究,发瑞 武汉地区板栗幼旺树一年具有多次开花结果的特性,一,二,三次花分别集中于５月下旬,７月上旬和８月上旬;并且一次花与二,三次的混合花序数量,着生部位及发育时间不等不同,表现为一次的混合花序主要着生在结果枝的上端,数量少,而二,三次花的混合花序从结果枝基部至顶均可形成,发育时间短,数量多,且二交错     

实验动物泰泽氏病原体检测

采用肝压印片、姬姆萨染色,对昆明小鼠、ＮＩＨ小鼠、ＬＩＢＰ／１小鼠、Ｗｉｓｔａｒ大鼠、豚鼠、金黄色地鼠、兔进行泰泽氏病原体调查,结果表明除兔以外各种动物,均发现有泰泽氏病原体感染,并以两种形式存在于肝组织中。第一种为典型毛发样杆菌,成束状排列;第二种为杆状或梭状,菌体长短粗细不一,分散于组织间。在肝压印片中也可见到菌体芽胞和游离芽胞。     

果园生态栽培及其生理生态效应研究进展

果园生态栽培及其生理生态效应研究进展周志翔李国怀徐永荣（华中农业大学,武汉４３００７０）ＯｒｃｈａｒｄＥｃｏｃｕｌｔｉｖａｔｉｏｎａｎｄｔｈｅＲｅｓｅａｒｃｈＡｄｖａｎｃｅｓｏｆＥｃｏｐｈｙｓｉｏｌｏｇｉｃａｌＣｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆＦｒｕ...     

Characterization of phytotoxin and secreted proteins identifies of Lasiodiplodia theobromae, causes of peach gummosis

Lasiodiplodia theobromae, a phytopathogenic fungus that causes peach gummosis, is considered one of the major constraints to peach production worldwide. Here, we report the characteristics of toxic metabolites and the proteomics investigation of the secreted proteins of L. theobromae from its in vitro culture. The phytotoxins of L. theobromae from the culture filtrate of Richard’s liquid medium showed high toxicity on peach current year shoots with large lesion diameter and high gum weight. The toxicity measurement showed that 23.6 and 21.2 mg gum were induced from peach shoots by solvent fractions of ethyl acetate and dichloromethane, respectively, with significant differences from other organic solvents. A total of 23 proteins were identified by liquid chromatography–mass spectrometry from the in vitro secretome of L. theobromae. Sequence analysis indicated that 14 proteins were extracellular proteins based on signal peptides and localization. The expression profiles of the analyzed fungal genes were significantly upregulated from 1 day postinoculation (dpi) to 2 dpi, indicating that the early stage is an important stage for the infection of L. theobromae. The present study has provided insights into the extracellular phytotoxins and secreted proteins that are possibly associated with pathogenicity of the peach gummosis.     

油菜基因流网络的结构特性与小团体分析

为从系统角度掌握转基因油菜（Brassica campestris）基因流规律,基于常规油菜与十字花科植物杂交的有关文献,构建了油菜基因流网络拓扑图实例并分析其网络结构特性。研究结果表明,该网络节点度服从幂律分布,具有无标度特性。从随机攻击和恶性攻击两个方面对标准结构熵和网络效率2个指标的网络稳健性进行分析,结果显示在随机移除不到10%的顶点时网络表现较好的鲁棒性,但在受到选择性移除10%的顶点时,网络具有极弱的抗攻击性。基于软件UCINET对网络进行了小团体和结构同型性分析,可将网络中298个节点22类十字花科植物划分为2个小团体和5类结构角色,其中甘蓝型油菜在基因流网络小团体中具有关键性作用。这些研究结果可为揭示转基因油菜基因流规律提供新思路,同时也可对转基因油菜商业化种植采取合理的农业生产栽培管理措施提供参考。