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1.
Actin-binding proteins involved in the capping of epidermal growth factor receptors in A431 cells. 总被引:3,自引:0,他引:3
K Kwiatkowska I A Khrebtukova D A Gudkova G P Pinaev A Sobota 《Experimental cell research》1991,196(2):255-263
A capping process of epidermal growth factor receptors (EGF-Rs) was used for the study of the relation between the receptors and the actin-binding proteins (spectrin, vinculin, annexin I) that may be involved in EGF-R-cytoskeleton interaction. In intact, adherent A431 cells, EGF-Rs were diffusively distributed on the cell surface. Spectrin, vinculin, and annexin I were located beneath the plasma membrane. An abundance of EGF-Rs as well as submembrane proteins was observed in regions of membrane ruffles and cell-cell contacts. Annexin I was localized also in cytoplasm being attached to filamentous structures surrounding the nucleus and extending to the cell periphery. Under polyvalent ligand treatment, EGF-Rs of adherent cells were aggregated on one side of the cell. Spectrin, vinculin, and annexin I dislocated together with EGF-Rs and were concentrated under plasma membrane at regions where cap formation took place. In suspended A431 cells only spectrin was located under the plasma membrane whereas annexin I and vinculin were diffusively distributed through the cells. During cap formation only spectrin was colocalized with EGF-Rs. The results confirmed the major role of spectrin as a receptor-microfilament linking protein. 相似文献
2.
Mateikovich A. P. Punina E. O. Kopylov-Guskov Yu. O. Nosov N. N. Gudkova P. D. Gnutikov A. A. Machs E. M. Mikhailova Yu. V. Krapivskaya E. E. Rodionov A. V. 《Russian Journal of Genetics》2020,56(4):417-428
Russian Journal of Genetics - The ITS1–5.8S rDNA–ITS2 sequence of the 35S rRNA genes of 16 species of feather grasses and 2 species of false needlegrasses of the flora of the Altai... 相似文献
3.
Bao Nie Bo-Han Jiao Li-Fei Ren Polina D. Gudkova Wen-Li Chen Wen-Hao Zhang 《植物分类学报:英文版》2022,60(4):901-913
Stipa shanxiensis, a cryptic species within Stipa grandis that originated from central and western China, is described based on morphological, genomic, and ecological data from field and common garden experiments. Stipa shanxiensis morphologically resembles S. grandis, although phylogenetically it is closely related to the less morphologically similar Stipa baicalensis and Stipa krylovii. Of the eight significant morphological differences between S. shanxiensis and S. grandis, the two, cauline ligules longer than 2 cm with a filiform apex, and hairs shorter than 0.2 mm on the adaxial surface of the cauline uppermost leaves can be used to distinguish the species. Results from a common garden experiment verified that the two diagnostic characteristics were relatively stable and less morphologically plastic in response to environmental variation. Furthermore, a significant ecological divergence was found between S. shanxiensis and S. grandis, such that the former preferred warmer and more humid climates, and their predicted distribution was generally separated. Taken together, our results highlight that the integrative taxonomic approach was valuable for recognizing a new cryptic species in Stipa. In particular, we find that common garden experiments involving the effects of growth stage and characteristic position helped to morphologically diagnose cryptic species. These findings may also facilitate our understandings of ecological adaption and phenotypic plasticity in response to environmental change. 相似文献
4.
K. V. Solovyov N. A. Grudinina E. N. Semernin I. V. Morozova S. A. Smirnova D. S. Polyakov T. D. Aleynikova E. V. Shliakhto A. Ya. Gudkova M. M. Shavlovsky 《Russian Journal of Genetics》2011,47(4):477-482
A search of transthyretin (TTR) gene mutations was performed in patients with cardiomyopathies from St. Petersburg. Mutations H90N, V30M and deletion (del9)
of nucleotides GACTTCTCC in position 6776 from the start codon of the TTR gene (in position 98782 according to reference sequence AC079096 (NCBI) was found. The H90N mutation in the third exon of
TTR gene was detected in a son of a cardiomyopathy patient and in his mother, which lacked any clinical manifestations. Mutation
V30M in exon 2 of TTR gene was found in heterozygous in one of the probands. Deletion (del9) was revealed in a patient with cardiomyopathy and
in his two daughters from different marriages, who had no clinical manifestations of the disease. All the mutations revealed
in this study were previously identified in other populations. 相似文献
5.
Lack of direct DNA damage in human blood leukocytes and lymphocytes after in vitro exposure to high power microwave pulses 总被引:3,自引:0,他引:3
Chemeris NK Gapeyev AB Sirota NP Gudkova OY Tankanag AV Konovalov IV Buzoverya ME Suvorov VG Logunov VA 《Bioelectromagnetics》2006,27(3):197-203
Currently, the potential genotoxicity of high power microwave pulses (HPMP) is not clear. Using the alkaline single cell gel electrophoresis assay, also known as the alkaline comet assay, we studied the effects of HPMP (8.8 GHz, 180 ns pulse width, peak power 65 kW, pulse repetition frequency 50 Hz) on DNA of human whole-blood leukocytes and isolated lymphocytes. The cell suspensions were exposed to HPMP for 40 min in a rectangular waveguide. The average SAR calculated from the temperature kinetics was about 1.6 kW/kg (peak SAR was about 300 MW/kg). The steady-state temperature rise in the 50 microl samples exposed to HPMP was 3.5 +/- 0.1 degrees C. In independent experiments, we did not find any statistically significant DNA damage manifested immediately after in vitro HPMP exposure of human blood leukocytes or lymphocytes or after HPMP exposure of leukocytes subsequently incubated at 37 degrees C for 30 min. Our results indicate that HPMP under the given exposure conditions did not induce DNA strand breaks, alkali-labile sites, and incomplete excision repair sites, which could be detected by the alkaline comet assay. 相似文献
6.
G R Matsevich M A Frolova R G Gudkova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1978,(4):24-26
Experiments were conducted on guinea pigs with the use of cell migration inhibition test of the peritoneal exudate; stimulation of a definite level of cell immunity in response to the administration of both live and of inactivated vaccine virus was shown. The results obtained are used for the interpretation of the action mechanism of the inactivated preparation in two-stage smallpox vaccination. 相似文献
7.
A E Oreshkin M V Gudkova N V Miasishcheva 《Biulleten' eksperimental'no? biologii i meditsiny》1992,114(8):185-187
The influence of mitogens on the expression of surface membrane TC-II receptors of human blood lymphocytes and internalization of (TS-II+57Co-CNCbl) complex into cytoplasm were investigated. Mature lymphocytes have a very small number of surface receptors to plasma TC-II but their expression is increased significantly by PHA or Con-A stimulation. CBl transport to cytoplasma is activated in definite sequence by two different mechanisms. Stimulated cells take free CBl without participation of TC-II in early hours of mitogen action (12-42 hrs) before maximal 3H-thymidine incorporation into DNA. On day 3 of cultivation, specific mechanism of CBl transport triggers and the number of lymphoblast receptors is increased manifold. Radioactive CBl enters cytoplasma due to interaction of TC-II-CN [57Co] CBl of the medium with surface membrane receptor of the cells. Thus, the definition of TC-II receptors as an important functional parameter may serve a marker of proliferating cells. 相似文献
8.
Dynamics of changes of and plasma corticosterone was studied in both male and female rats after intraperitoneal injections of adrenaline at a dose of 20 micrograms per 100 g body weight. The control rats were injected with saline. Animals were decapitated 10, 30, 60, 120, 180 and 240 min after the injections. The specific effect of adrenaline was revealed in the first 10 min of adrenaline injection. This effect was significantly increased to 30-60 min after termination of saline--induced activation of the pituitary-adrenal axis. Both saline and adrenaline caused more significant increases in corticosterone levels in female rats than in male ones. There was a significant delay in the return of corticosterone to resting levels in males compared to that in females. It is supposed that the almost two-fold difference in peak plasma corticosterone concentrations observed after stressors may be associated with increased responsiveness of the female hypothalamus with respect to adrenaline secretion. 相似文献
9.