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排序方式: 共有218条查询结果,搜索用时 31 毫秒
1.
F Flamigni S Marmiroli C Guarnieri C M Caldarera 《Biochemical and biophysical research communications》1989,163(3):1217-1222
Ornithine decarboxylase activity in Friend erythroleukemia cells decayed with a half-life of 50 minutes after addition of cycloheximide and at a faster rate after addition of spermidine. Incubation with a medium containing dinitrophenol and 2-deoxy-glucose in place of glucose caused ATP depletion and blocked the turnover of ornithine decarboxylase, even after addition of spermidine. Dinitrophenol in the presence of glucose was able to provoke only a slight increase of the half-life of the enzyme. These results suggest that degradation of ornithine decarboxylase in erythroleukemia cells is ATP-dependent. 相似文献
2.
C Guarnieri A Georgountzos I Caldarera F Flamigni A Ligabue 《Biochimica et biophysica acta》1987,930(2):135-139
The polyamines putrescine, spermidine and spermine, at concentrations of 10 microM, stimulated superoxide generation by human polymorphonuclear leukocytes induced by fMet-Leu-Phe in the presence of Ca2+. This positive effect was not evident in the absence of Ca2+ or when the polymorphonuclear leukocytes were stimulated by phorbol myristate acetate. Spermidine in the range of 10-100 microM showed a dose-dependent stimulatory effect on the superoxide generation induced by fMet-Leu-Phe, whilst at doses above 25 mM it produced an inhibitory effect. At this concentration, spermidine did not reduce the phorbol myristate acetate-neutrophil-induced O2-. generation, while an inhibitory effect by the polyamine was evident at concentrations above 50 mM. In addition, 100 microM spermidine increased the amount of superoxide generated and enhanced the ability of the chemotactic peptide to stimulate superoxide generation. The polyamines in the range of 10 microM-25 mM did not modify the activity of purified NADPH oxidase, nor the rate of reduction of cytochrome c as supported by the xanthine/xanthine oxidase reaction. These results indicate that physiological concentrations of polyamines can stimulate superoxide formation by polymorphonuclear leukocyte cells produced by the chemotactic peptide fMet-Leu-Phe, probably by increasing the availability of external calcium. 相似文献
3.
Dr. Edward B. Blanchard James Jaccard Frank Andrasik Patricia Guarnieri Susan E. Jurish 《Applied psychophysiology and biofeedback》1985,10(1):63-68
Comparisons are made of self-reported medical costs from a sample of headache patients who underwent various combinations of relaxation training and biofeedback training. The average costs for the 2 years prior to self-regulatory treatment were $955±480 (3 SEM) for 45 patients; for the 2 years after completing treatment the average costs were $52±28 (3 SEM) for patients. Within the limitations of the study, medical costs do seem to have been markedly reduced.This research was supported by a grant from NINCDS, NS-15235. 相似文献
4.
NADH-dependent formation of superoxide anions (O-2) by rabbit cardiac submitochondrial particles (SMP) was stimulated after exposure of the isolated heart to 90 min of ischemic perfusion. This effect was more evident in the rotenone-inhibited region of the respiratory electron chain in comparison to the antimycin-inhibited region. The kinetic study of the NADH-dependent reaction showed that at the level of the rotenone-inhibited region, ischemia reduced Km value for NADH, differently from the antimycin-inhibited region where the kinetic constants remain unchanged. No significant changes of the Vmax values were observed in both SMP-producing O-2 sites.
The ischemic perfusions also produced a reduction of mitochondrial function, particularly evident when glutamate as substrate was studied. 相似文献
The ischemic perfusions also produced a reduction of mitochondrial function, particularly evident when glutamate as substrate was studied. 相似文献
5.
Joan E. Kuster Mary H. Guarnieri Jeffrey G. Ault Lorraine Flaherty Pamela J. Swiatek 《Mammalian genome》1997,8(9):673-681
The laminin-5 molecule functions in the attachment of various epithelia to basement membranes. Mutations in the laminin-5-coding
genes have been associated with Herlitz junctional epidermolysis bullosa (HJEB), a severe and often lethal blistering disease
of humans. Here we report the characterization of a spontaneous mouse mutant with an autosomal recessive blistering disease.
These mice exhibit sub-epithelial blisters of the skin and mucosal surfaces and abnormal hemidesmosomes lacking sub-basal
dense plates. By linkage analysis the genetic defect was localized to a 2-cM region on distal Chromosome (Chr) 1 where a laminin-5
subunit gene, LamB3, was previously localized. LamB3 mRNA and laminin-5 protein were undetectable by Northern blot analysis and immunohistochemical methods, respectively. DNA
sequence analysis indicated that the LamB3 genetic defect resulted from disruption of the coding sequence by insertion of an intracisternal-A particle (IAP) at an exon/intron
junction. These findings suggest a role for laminin-5 in hemidesmosome formation and indicate that the LamB3
IAP
mutant is a useful mouse model for HJEB.
Received: 27 March 1997 / Accepted: 14 May 1997 相似文献
6.
C. Guarnieri G. Zucchelli F. Bernardi M. Scheda A. F. Valentini M. Calandriello 《Free radical research》1991,15(1):11-16
In the gingival crevicular fluid (GCF) of control and chronic adult periodontitis (CAP) patients there is a spontaneous release of O2- radicals from polymorphonuclear leukocytes (PMN). The addition of the exogenous stimuli phorbol myrystate acetate (PMA) decreased the O2- formation in control GCF, while in CAP patients produced a marked enhancement of O2- generation.
The circulating PMN of control subjects did not show a spontaneous O2- formation, differently from CAP patients. On the contrary, a similar O2- production was measured when the circulating PMN were stimulated with PMA.
Moreover, the antioxidant activity measured in 10μl of cell free gingival supernatant (GS) of control and CAP patients had the same values by inhibiting 12.6% and 18.9% respectively of the O2- formation supported by a xanthine/xanthine oxidase system.
Probably, the protective or destructive effect of PMN in GCF of CAP patients depends on the variations of the rate of O2- formation in respect to the intrinsic antioxidant property of GS. 相似文献
The circulating PMN of control subjects did not show a spontaneous O2- formation, differently from CAP patients. On the contrary, a similar O2- production was measured when the circulating PMN were stimulated with PMA.
Moreover, the antioxidant activity measured in 10μl of cell free gingival supernatant (GS) of control and CAP patients had the same values by inhibiting 12.6% and 18.9% respectively of the O2- formation supported by a xanthine/xanthine oxidase system.
Probably, the protective or destructive effect of PMN in GCF of CAP patients depends on the variations of the rate of O2- formation in respect to the intrinsic antioxidant property of GS. 相似文献
7.
F Flamigni S Marmiroli C Guarnieri C M Caldarera 《Biochemical and biophysical research communications》1990,172(2):939-944
Addition of spermidine to Friend erythroleukemia cells caused a rapid decay of ornithine decarboxylase (ODC) activity and the accumulation of a ODC-antizyme complex. The induction of antizyme only partially accounted for the decrease of ODC activity by a direct inhibition of the enzyme. However, the antizyme induction was accompanied by a marked reduction of the half-life of ODC. Shift of the cells to an ATP-depleting medium prevented the spermidine-elicited decay of ODC activity as well as the accumulation of ODC-antizyme complex. However, ODC appeared to be stabilized even when ATP depletion was performed 40 min after spermidine addition, in the presence of high levels of antizyme. Similar results were obtained by treating the cells with phenanthroline, a heavy metal chelator and protease inhibitor. These findings indicate that ATP and some metalloprotease(s) may be involved in the degradation pathway of ODC, even in the presence of high levels of polyamines. 相似文献
8.
Glutathione peroxidase activity and release of glutathione from oxygen-deficient perfused rat heart.
C Guarnieri F Flamigni C Rossoni-Caldarera 《Biochemical and biophysical research communications》1979,89(2):678-684
The effect of cellular hypoxia on glutathione levels in rat hearts was determined. Hearts perfused with 95% N2–5% CO2 demonstrated a significant decrease in tissue reduced glutathione content when compared to control hearts perfused with 95% O2–5% CO2. The hypoxic perfusate contained reduced glutathione and its release was time dependent over a period of 60 minutes. The cellular depletion of oxidized glutathione and its release into coronary effluent were less evident with respect to reduced glutathione. Moreover during hypoxic perfusion we have observed a decrease of cytosol glutathione peroxidase activity. These results suggest that severe oxygen-deprivation causes in myocardial cells a significant perturbation of glutathione metabolism. 相似文献
9.
E B Blanchard J Jaccard F Andrasik P Guarnieri S E Jurish 《Biofeedback and self-regulation》1985,10(1):63-68
Comparisons are made of self-reported medical costs from a sample of headache patients who underwent various combinations of relaxation training and biofeedback training. The average costs for the 2 years prior to self-regulatory treatment were $955 +/- 480 (3 SEM) for 45 patients; for the 2 years after completing treatment the average costs were $52 +/- 28 (3 SEM) for patients. Within the limitations of the study, medical costs do seem to have been markedly reduced. 相似文献
10.
The initial time and rate of myelin basic protein synthesis in neural tissues of the rat have been measured from birth to 120 days. The protein was quantitated by a radioimmunoassay directly applied to unfractionated cerebrum, cerebellum, olfactory bulb, midbrain, brain stem, optic and trigeminal nerve, and areas of the spinal cord. Because the protein is a specific myelin constituent and its appearance correlates precisely with the synthesis of myelin lipids, the data in this report can be interpreted in terms of myelin synthesis and oligodendrocyte activity. The results show striking heterogeneity in the initial time and rate of myelin synthesis in neural tissue. 相似文献